【摘要】：羥基磷灰石(HA), Ca10(PO4)6(OH)2,哺乳動(dòng)物體內(nèi)骨骼、牙齒等硬組織的主要無機(jī)成分,有良好的骨傳導(dǎo)性和骨誘導(dǎo)性。聚乳酸(PLLA),在為數(shù)眾多的聚合物中被證明為最具研究熱點(diǎn)的可降解聚多酯,具有良好的生物可降解性、生物相容性和機(jī)械性能,通過生物體可被降解為體內(nèi)三羧酸循環(huán)產(chǎn)物。本文主要研究?jī)煞NHA的表面改性方法和制備了羥基磷灰石/聚乳酸復(fù)合材料,這種材料兼具了二者的優(yōu)點(diǎn),既具有生物降解性又具有骨傳導(dǎo)性,可應(yīng)用于骨組織工程支架材料中。 1.聚L-苯丙氨酸(PPha)對(duì)羥基磷灰石表面改性。首先,我們通過HA和γ-氨丙基三乙氧基硅烷反應(yīng)得到表面氨基化的羥基磷灰石(HAAPS)。其次,HAAPS引發(fā)L-苯丙氨酸的N-羰基環(huán)內(nèi)酸酐開環(huán)聚合得到表面接枝聚L-苯丙氨酸的羥基磷灰石。合成結(jié)果通過FTIR, TGA, XRD表征。通過控制不同的反應(yīng)時(shí)間和比例,HA表面的聚L-苯丙氨酸(PPha-g-HA)接枝率為20.26%-38.92%。XRD分析證實(shí)經(jīng)過表面改性的HA并沒很好的有改變HA的專屬性能。MTT顯示PPha-g-HA具有很好的生物相容性,在骨組織工程具有很好的應(yīng)用前景。 2.聚(Nε-芐氧羰基-L-賴氨酸)(PLys(z))對(duì)羥基磷灰石的表面改性。將制得的HAAPS引發(fā)Nε-芐氧羰基-L-賴氨酸的N-羰基環(huán)內(nèi)酸酐(Lys(z)-NCA)開環(huán)聚合得到表面接枝聚(Nε-芐氧羰基賴氨酸)的HA (Poly(N-ε-Z-L-Lysine)-g-HA)(PLys(z)-g-HA)。合成過程通過FTIR, TGA, XRD檢測(cè),相較于HA和HAAPS,紅外圖中表面接枝聚Nε-芐氧羰基賴氨酸羥基磷灰石PLys(z)-g-HA在1649cm-1和1709cm-1多出了兩個(gè)吸收峰,分別為酰胺Ⅰ帶的羰基振動(dòng)峰和酯基的羰基振動(dòng)峰,從一方面證明了聚(Nε-芐氧羰基-L-賴氨酸)接枝在了HA表面。同時(shí),從TGA的數(shù)據(jù)分析中可以看到通過控制不同的反應(yīng)時(shí)間和比例,PLys(z)-g-HA接枝率為5.9%至28.99%進(jìn)一步論證了我們的結(jié)論。從XRD和MTT中可以得出(PLys(z)-g-HA)沒有改性HA的專屬性能和無毒性,甚至在低濃度的情況下可以促進(jìn)細(xì)胞的增殖。 3.PLys(z)-g-HA和聚L-乳酸(PLLA)復(fù)合材料的制備。采用相分離的方法制備了不同PLys(z)-g-HA濃度的復(fù)合材料。DSC用來表征PLys(z)-g-HA對(duì)PLLA結(jié)晶性能的影響。從PLLA和PLys(z)-g-HA/PLLA復(fù)合材料的熱分析表明,PLys(z)-g-HA可以提高PLLA的熱學(xué)穩(wěn)定性能；改性的PLys(z)-g-HA在復(fù)合材料中起到成核劑的作用,能夠加快PLLA的結(jié)晶速度。
[Abstract]:Hydroxyapatite (HA), Ca10 (PO4) 6 (OH) 2 is the main inorganic component of hard tissues such as bone and teeth in mammals. It has good bone conductivity and bone induction. Polylactic acid (PLLA),) has been proved to be the most popular biodegradable polyester among many polymers. It has good biodegradability, biocompatibility and mechanical properties. It can be degraded into tricarboxylic acid cycle product by organism. In this paper, the surface modification methods of two kinds of HA and the preparation of hydroxyapatite / polylactic acid composite were studied. This material has the advantages of both biodegradability and bone conductivity. It can be used in bone tissue engineering scaffold material. 1. Surface modification of hydroxyapatite by poly (L-phenylalanine) (PPha). First of all, we obtained the surface amino hydroxyapatite (HAAPS). By the reaction of HA and 緯 -aminopropyl triethoxy silane. Secondly, HAAPS initiated ring-opening polymerization of L-phenylalanine with N-carbonyl anhydride to obtain surface grafted L-phenylalanine hydroxyapatite. The synthesis results were characterized by FTIR, TGA, XRD. The 20.26%-38.92%.XRD analysis of poly (L-phenylalanine) (PPha-g-HA) grafted on HA with different reaction time and ratio showed that the modified HA did not change the specific properties of HA. The results showed that PPha-g-HA had good biocompatibility. It has a good application prospect in bone tissue engineering. 2. Surface Modification of hydroxyapatite by Poly (N 蔚-benzoxycarbonyl-L-lysine) (PLys (z). The HAAPS initiated ring opening polymerization of N 蔚 -benzyloxycarbonyl-L-lysine (N-carbonyl anhydride (Lys (z) -NCA) to obtain HA (Poly (N- 蔚 -Z-L-Lysine) -g-HA) (PLys (z) -g-HA on the surface of N 蔚 -benzyloxycarbonyl lysine. Compared with the surface grafted N 蔚 benzoxy carbonyl lysine hydroxyapatite PLys (z) g-HA in HA and HAAPS, infrared images, there are two absorption peaks in 1649cm-1 and 1709cm-1, which are the carbonyl vibrational peaks of amide I band and the carbonyl vibrational peak of ester group, respectively. On one hand, it is proved that poly (N 蔚 -benzyloxycarbonyl-L-lysine) grafted on the surface of HA. At the same time, from the data analysis of TGA, it can be seen that our conclusion is further demonstrated by controlling the different reaction time and proportion of PLys (z) -g-HA grafting rate ranging from 5.9% to 28.99%. From XRD and MTT, it can be concluded that (PLys (z) -g-HA has no specific property and nontoxicity of modified HA, and can even promote cell proliferation at low concentration. The preparation of 3.PLys (z) -g-HA and Poly (L-lactic acid) (PLLA) composites. The composites with different PLys (z) concentration were prepared by phase separation method. The effect of PLys (z) on the crystallization properties of PLLA was investigated. The thermal analysis of PLLA and PLys (z) -g-HA / PLLA composites shows that PLLA (z) -g-HA can improve the thermal stability of PLLA, and the modified PLys (z) -g-HA acts as a nucleating agent in the composites, which can accelerate the crystallization rate of PLLA.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08

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本文編號(hào)：2218830