本文選題：雙相磷酸鈣陶瓷 + 多孔�。� 參考：《華南理工大學(xué)》2015年碩士論文

【摘要】：本文針對(duì)目前冷凍干燥法制備的定向多孔磷酸鈣陶瓷材料在孔隙寬度和力學(xué)性能上存在的不足,采用冰晶模板-冷凍干燥法制備了具有定向多孔結(jié)構(gòu)的雙相磷酸鈣(BCP)陶瓷支架材料。通過(guò)優(yōu)化燒成制度;加入Na Cl等添加劑調(diào)控孔結(jié)構(gòu);灌注卵磷脂改性PLGA溶液和添加磷酸鹽玻璃(PG)燒結(jié)助劑兩種方法對(duì)支架進(jìn)行增強(qiáng),并評(píng)價(jià)增強(qiáng)支架的細(xì)胞行為,從而得到了結(jié)構(gòu)和性能較好的多孔孔雙相磷酸鈣陶瓷支架。通過(guò)將中位徑為7.554μm的β-TCP粉末和中位徑為12.024μm的HA粉末在去離子水中機(jī)械混合制備了較為均勻的BCP料漿,灌注后采用冰晶模板-冷凍干燥法制備了具有定向多孔結(jié)構(gòu)的雙相磷酸鈣陶瓷材料。通過(guò)探究燒成溫度(1000~1300°C)和保溫時(shí)間(2、4、6 h)對(duì)支架物相組成、多孔結(jié)構(gòu)以及抗壓強(qiáng)度的影響,優(yōu)化了支架的燒成制度。通過(guò)在制備料漿時(shí)添加Na Cl溶液顯著增大了定向多孔BCP支架垂直于冷凍方向上的孔隙尺寸。當(dāng)Na Cl的添加量為10 wt.%時(shí),支架垂直于冷凍方向上的平均孔徑可達(dá)111.27μm,支架的抗壓強(qiáng)度也得到顯著提高。實(shí)驗(yàn)還發(fā)現(xiàn),通過(guò)在料漿中加入聚乙二醇(PEG)、聚乙烯吡咯烷酮(PVP)并不能促進(jìn)冰晶尺寸的增大從而得到較大孔徑的多孔支架。采用添加磷酸鹽生物玻璃(PG)作燒結(jié)助劑和灌注卵磷脂改性的PLGA溶液復(fù)合的兩種增強(qiáng)方法,通過(guò)探究PG的添加量和PLGA溶液的灌注濃度,制備得到了具有良好結(jié)構(gòu)和性能的多孔支架材料。當(dāng)PG添加量為17.5 wt.%時(shí),支架的抗壓強(qiáng)度可達(dá)5.7 MPa,孔隙率為55.9%;當(dāng)PLGA溶液的復(fù)合濃度為7.5%時(shí),顯著提高了支架的韌性,形變量和抗壓強(qiáng)度分別可以達(dá)到40%和7.8 MPa,孔隙率為57.3%。同時(shí),對(duì)增強(qiáng)支架的體外降解性能和細(xì)胞生物學(xué)行為的研究結(jié)果表明PG/BCP和PLGA/BCP多孔支架材料在PBS中浸泡28天后失重率都較低(10%),沒(méi)有發(fā)生崩解,能保持原有的結(jié)構(gòu),兩種材料都具有良好的生物相容性,細(xì)胞在材料上都黏附良好,且具有較好的增殖能力,有望經(jīng)后續(xù)改進(jìn)后應(yīng)用于骨缺損修復(fù)領(lǐng)域。
[Abstract]:In this paper, the defects in pore width and mechanical properties of directional porous calcium phosphate ceramics prepared by freeze-drying method are pointed out. A biphasic calcium phosphate (BCP) ceramic scaffold with directional porous structure was prepared by freezing drying method. The scaffold was enhanced by optimizing sintering system, adding NaCl and other additives to regulate pore structure, injecting lecithin modified PLGA solution and adding phosphate glass (PG) sintering promoter to enhance the scaffold, and to evaluate the cell behavior of the enhanced scaffold. Thus, porous dual phase calcium phosphate ceramic scaffolds with better structure and performance were obtained. By mixing 尾 -TCP powder with a median diameter of 7.554 渭 m and HA powder with a median diameter of 12.024 渭 m in deionized water, a uniform BCP slurry was prepared. After perfusion, biphasic calcium phosphate ceramics with directional porous structure were prepared by freezing drying method. The sintering process of the scaffold was optimized by investigating the effects of sintering temperature (1000 ~ 1300 擄C) and holding time (2 ~ 4 ~ 6 h) on the phase composition, porous structure and compressive strength of the scaffold. The pore size perpendicular to the freezing direction of the directional porous BCP scaffold was significantly increased by adding NaCl solution in the preparation of slurry. The average pore diameter perpendicular to the freezing direction can reach 111.27 渭 m when the NaCl content is 10 wt.%, and the compressive strength of the scaffold is also significantly increased. It was also found that polyvinylpyrrolidone (PVP) could not promote the increase of ice crystal size by adding polyethylene glycol (PEG) to the slurry to obtain porous scaffolds with large pore size. By adding phosphate bioglass (PG) as sintering promoter and by adding lecithin modified PLGA solution, two reinforcement methods were used to study the dosage of PG and the concentration of PLGA solution. Porous scaffolds with good structure and properties were prepared. When PG was added 17.5 wt.%, the compressive strength and porosity of the scaffold reached 5.7 MPA and 55.90.When the composite concentration of PLGA solution was 7.5, the toughness of the scaffold was significantly improved, the shape variable and compressive strength were 40% and 7.8 MPA, respectively, and the porosity was 57.3 MPA. At the same time, the results of in vitro degradation and cell biological behavior of enhanced scaffolds showed that the weight loss rate of PG- / BCP and PLGA / BCP porous scaffolds after 28 days of immersion in PBS was lower (10%), no disintegration occurred, and the original structure could be maintained. The two materials have good biocompatibility, good adhesion on the material, and good proliferative ability. They are expected to be used in the field of bone defect repair after further improvement.
【學(xué)位授予單位】：華南理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類(lèi)號(hào)】：R318.08

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本文編號(hào)：2110840