發(fā)布時間：2018-07-09 11:42

  本文選題：靜電紡絲 + 聚碳酸酯聚氨酯　； 參考：《天津大學》2013年碩士論文

【摘要】：隨著組織工程的發(fā)展，發(fā)揮靜電紡絲技術的獨特優(yōu)勢，，制備具有合適物理機械性能的仿生支架作為小口徑血管成為研究熱點。本文利用靜電紡絲技術制備聚碳酸酯聚氨酯三維纖維小孔徑血管支架，并通過聚合接枝改性方法對支架的表面進行了改性，使其具有良好的生物相容性以用作小口徑血管支架。首先利用靜電紡絲技術制備了形貌結構良好的PCU纖維支架，為了改善PCU疏水性的缺點，利用表面引發(fā)原子轉移自由基聚合(SI-ATRP)方法在纖維表面接枝親水性材料聚乙二醇甲基丙烯酸酯(PEGMA)，并設計了由控制單體的投入量來控制鏈段的長度，并探究了鏈段長度對改性效果的影響。為了提高PCU纖維支架表面的生物形容性，本文又利用戊二醛交聯(lián)接枝方法將明膠這一天然生物蛋白質高分子引入到了纖維表面，并將PEGMA作為中間臂，將其羥基位點轉化為接枝明膠活性位點，放大了明膠的接枝密度，并進一步比較探討了明膠接枝量對其改性效果的影響。 采用SEM觀察改性過程對電紡絲骨架結構形貌的影響；通過水接觸角實驗來表征支架表面的親水性能；利用傅里葉紅外光譜(FT-IR)、X射線光電子能譜(XPS)檢測方法，表征改性前后樣品的化學性質的變化，從化學組成角度表征改性過程對材料表面性質的影響；利用血小板吸附實驗、溶血測試和活化部分凝血酶時間(APTT)測試，表征改性樣品血液相容性的變化；通過接種培養(yǎng)臍靜脈內皮細胞來表征改性支架的細胞相容性。 實驗結果表明各種改性方法在均沒有破壞電紡血管支架的骨架結構，并有效地改善了支架的血液相容性和生物相容性。與改性前相比，改性支架表面的親水性得到了明顯改善，且能有效抑制血小板粘附，溶血率也有所降低，APTT時間有所延長，表明改性支架具有良好的血液相容性。內皮細胞在改性后支架表面粘附、生長、增殖良好，能在表面形成單層細胞層。通過研究，本文改性的電紡支架為作為小口徑血管提供了可能。
[Abstract]:With the development of tissue engineering, the preparation of bionic scaffolds with suitable physical and mechanical properties as small diameter vessels has become a research hotspot. In this paper, polycarbonate polyurethane three-dimensional fiber scaffolds with small pore diameter were prepared by electrospinning technique. The surface of the scaffolds was modified by polymerization and graft modification, which made them have good biocompatibility to be used as small diameter vascular scaffolds. In order to improve the hydrophobicity of PCU, PCU fiber scaffolds with good morphology and structure were prepared by electrostatic spinning. Surface initiated atom transfer radical polymerization (SI-ATRP) was used to graft the hydrophilic polyethylene glycol methacrylate (PEGMA) onto the fiber surface, and the length of the chain was controlled by controlling the amount of monomer input. The effect of chain length on the modification effect was also investigated. In order to improve the biological description of the surface of PCU fiber scaffold, gelatin, a natural biological protein polymer, was introduced into the fiber surface by glutaraldehyde crosslinking grafting method, and PEGMA was used as the intermediate arm. The hydroxyl site was transformed into the active site of grafted gelatin, the grafting density of gelatin was enlarged, and the effect of the amount of gelatin graft on the modification effect was discussed. SEM was used to observe the influence of the modification process on the morphology of the electrospinning skeleton structure, the hydrophilicity of the scaffold surface was characterized by water contact angle experiment, and the X-ray photoelectron spectroscopy (XPS) method was used to detect the structure of the scaffold by Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). The changes of the chemical properties of the samples before and after modification were characterized, and the effects of the modification process on the surface properties of the materials were characterized from the perspective of chemical composition, and the platelet adsorption test, hemolysis test and activated partial thrombin time (APTT) test were used. The changes of blood compatibility of the modified scaffolds were characterized by inoculation of umbilical vein endothelial cells (HUVEC) to characterize the cytocompatibility of the modified scaffolds. The experimental results show that all kinds of modification methods do not destroy the skeleton structure of electrospun vascular scaffolds and improve the blood compatibility and biocompatibility of the scaffolds effectively. Compared with before modification, the hydrophilicity of the modified scaffold was obviously improved, and the platelet adhesion was effectively inhibited, and the hemolysis rate was also decreased. The results showed that the modified scaffold had good blood compatibility. Endothelial cells adhered, grew and proliferated well on the surface of the scaffold, and formed monolayer cell layer on the surface. Through the research, the modified electrospinning scaffold provides the possibility for the small diameter blood vessel.
【學位授予單位】：天津大學
【學位級別】：碩士
【學位授予年份】：2013
【分類號】：R318.08

【參考文獻】

相關期刊論文 前1條

1 孟慶圓;崔福齋;朱寧;王小平;;電紡絲方法制備卵磷脂改性聚乳酸血管組織工程支架材料[J];生物骨科材料與臨床研究;2009年04期

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