發(fā)布時(shí)間：2018-07-08 21:17

  本文選題：契倫科夫光學(xué)成像 + 四環(huán)素��； 參考：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2017年22期

【摘要】：目的探究四環(huán)素、熒光素鈉、異硫氰酸熒光素(fluorescein isothiocyanate,FITC)這3種臨床可用的熒光材料對(duì)契倫科夫光學(xué)信號(hào)增強(qiáng)的效果及最佳應(yīng)用方案。方法將不同濃度的四環(huán)素溶液(100、50、10、5、1、0.5、0.1、0.05、0.01 mmol/L)、FITC溶液(5、4、3、2.5、1.5、1、0.5、0.25 mmol/L)和熒光素鈉溶液(100、50、10、5、2、1、0.2、0.1 mmol/L)分別與100μCi的~(18)F-FDG混合后進(jìn)行契倫科夫光學(xué)成像(Cerenkov luminescence imaging,CLI),觀察契倫科夫光學(xué)信號(hào)的增強(qiáng)效果,并勾畫感興趣區(qū)域(region of interest,ROI),對(duì)光學(xué)信號(hào)強(qiáng)度進(jìn)行定量分析,確定最佳信號(hào)增強(qiáng)效果的濃度,并對(duì)信號(hào)最強(qiáng)的板孔以20 nm為間隔進(jìn)行500~800 nm范圍的光譜分離成像。同時(shí)進(jìn)行在體實(shí)驗(yàn)驗(yàn)證熒光素鈉及FITC對(duì)契倫科夫光的增強(qiáng)效果。結(jié)果不同濃度的四環(huán)素溶液對(duì)光學(xué)信號(hào)無(wú)明顯影響。FITC及熒光素鈉均能顯著提高契倫科夫光學(xué)信號(hào)強(qiáng)度。當(dāng)FITC及熒光素鈉濃度分別為1或2 mmol/L時(shí)產(chǎn)生的光學(xué)信號(hào)強(qiáng)度最強(qiáng),為單獨(dú)的~(18)F-FDG的3.2倍及5倍。光譜分析表明2 mmol/L的熒光素鈉能將~(18)F-FDG產(chǎn)生的契倫科夫光(cerenkov luminescence,CL)光紅移至540 nm附近。荷瘤鼠在體實(shí)驗(yàn)表明注射FITC或熒光素鈉后能使腫瘤部位的CL信號(hào)強(qiáng)度提高2倍或3.9倍。結(jié)論 FITC、熒光素鈉均能通過契倫科夫光的激發(fā)效應(yīng)來(lái)增強(qiáng)契倫科夫光學(xué)信號(hào)強(qiáng)度,提高組織穿透力。
[Abstract]:Objective to investigate the effect of tetracycline, sodium fluorescein and fluorescein isothiocyanate on the enhancement of Cherenkov optical signal. Methods the different concentrations of tetracycline solution (100 ~ (50) C ~ (10) ~ (0.5) ~ (18) F-FDG) were mixed with 100 渭 ci ~ (18) F-FDG to observe the enhancement effect of Cerenkov luminescence imagingCLI, and the enhancement effect of Cerenkov luminescence imagingCLI was observed by observing the enhancement effect of Cerenkov optical signal, respectively, after mixing 100 渭 ci ~ (18) F-FDG with 100 ~ (50) 渭 ci ~ (18) F-FDG, respectively, and sodium fluorescein (100 ~ (50) 105210.22 ~ (0.1) mmol 路L ~ (-1) ~ (18) F ~ (18) F-FDG), respectively, in order to observe the enhancement of Cerenkov luminescence imagingCLI, and to observe the enhancement effect of Cerenkov luminescence imagingin CLI (100 ~ (18) F-FDG). The region of interest (region of) was drawn, the intensity of optical signal was quantitatively analyzed, the concentration of the best signal enhancement effect was determined, and the spectral separation imaging of the strongest plate hole was carried out in the range of 500-800 nm at an interval of 20 nm. At the same time, the enhancement effect of fluorescein sodium and FITC on Cherenkov light was tested in vivo. Results different concentrations of tetracycline solution had no significant effect on optical signal. FITC and sodium fluorescein could significantly improve the intensity of Cherenkov optical signal. When the concentration of FITC and sodium fluorescein was 1 or 2 mmol / L, the intensity of optical signal was the strongest, which was 3.2 and 5 times of that of ~ (18) F-FDG alone. Spectral analysis shows that 2 mmol / L sodium fluorescein can shift the cerenkov luminescence CL produced by ~ (18) F-FDG to about 540nm. In vivo experiments in tumor-bearing mice showed that FITC or sodium fluorescein could increase the signal intensity of CL in tumor site by 2 times or 3.9 times after injection of FITC or sodium fluorescein. Conclusion both FITC and sodium fluorescein can enhance the intensity of optical signal and enhance the penetration of tissue through the excitation effect of Cherenkov light.
【作者單位】： 武警總醫(yī)院消化內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金面上項(xiàng)目(81471700)~~
【分類號(hào)】：R817

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