發(fā)布時間：2018-07-04 19:53

  本文選題：多核巨細(xì)胞 + 骨橋蛋白　； 參考：《口腔醫(yī)學(xué)研究》2017年11期

【摘要】：目的:比較醫(yī)用純鈦和聚甲基丙烯酸甲酯(PMMA)2種材料-骨界面多核巨細(xì)胞分布情況和骨橋蛋白表達(dá)情況。方法:將醫(yī)用純鈦和PMMA制成的種植體各30枚植入30只SD大鼠的兩側(cè)股骨遠(yuǎn)端髁突內(nèi),分別于3、7、14、21和28d時處死動物。制備脫鈣石蠟切片,HE染色觀察種植體-骨間隙愈合界面多核巨細(xì)胞的分布;免疫組織化學(xué)法檢測種植體-骨界面骨橋蛋白的表達(dá)情況。結(jié)果:HE結(jié)果顯示在5個時間點里均可以觀察到種植體-骨界面有多核巨細(xì)胞的形成,3d時達(dá)到高峰且PMMA組多核巨細(xì)胞的數(shù)量明顯多于鈦組(P0.05);多核巨細(xì)胞數(shù)目隨時間推移均呈現(xiàn)下降趨勢,在21d和28d時無統(tǒng)計學(xué)差異。骨橋蛋白的表達(dá)在7d時達(dá)到高峰期,且醫(yī)用純鈦組明顯強(qiáng)于PMMA組(P0.05)。隨時間延長,骨橋蛋白在鈦組和PMMA組的表達(dá)均逐漸減弱。結(jié)論:不同骨內(nèi)植入材料影響其界面的多核巨細(xì)胞分布與骨橋蛋白的表達(dá)。
[Abstract]:Aim: to compare the distribution of multinucleated giant cells and osteopontin expression between pure titanium and polymethyl methacrylate (PMMA). Methods: 30 implants made of pure titanium and PMMA were implanted into the distal femoral condyle of 30 SD rats. The distribution of multinucleated giant cells in implant-bone gap healing interface was observed by HE staining and the expression of osteopontin in implant-bone interface was detected by immunohistochemical method. Results the size of multinucleated giant cells in the implant-bone interface reached a peak at 3 days after the implantation, and the number of multinucleated giant cells in the PMMA group was significantly higher than that in the titanium group (P0.05), and the number of multinucleated giant cells in the PMMA group was significantly higher than that in the titanium group (P0.05). All of them showed a downward trend. There was no statistical difference between 21 d and 28 d. The expression of osteopontin reached its peak at 7 days, and the medical pure titanium group was significantly stronger than PMMA group (P0.05). Over time, the expression of osteopontin in both titanium and PMMA groups decreased gradually. Conclusion: the distribution of multinucleated giant cells and the expression of osteopontin were affected by different intraosseous implants.
【作者單位】： 武漢大學(xué)口腔醫(yī)學(xué)院;武漢大學(xué)口腔醫(yī)院種植科;
【基金】：國家自然科學(xué)基金項目資助(編號:31570982) 武漢大學(xué)校級大學(xué)生創(chuàng)新創(chuàng)業(yè)訓(xùn)練項目(編號:S2016903)
【分類號】：R783.1

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