本文選題：硅基納米材料 + 表面增強拉曼散射�。� 參考：《蘇州大學(xué)》2012年碩士論文

【摘要】：硅納米材料是一種重要的半導(dǎo)體納米材料，它具有優(yōu)異的電學(xué)和機械性能、良好的生物相容性、較大的比表面積、表面易修飾性，以及與傳統(tǒng)的硅科技的兼容性。因此，人們對發(fā)展功能化的硅納米材料有著濃厚的興趣，并已將其廣泛應(yīng)用于電子學(xué)和生物學(xué)領(lǐng)域。本文主要發(fā)展了基于硅基納米材料的表面增強拉曼散射（surface-enhanced Raman scattering, SERS）基質(zhì)，并用于DNA的高靈敏、特異性多元檢測；還研究了硅納米線在細胞層面的生物學(xué)效應(yīng)，主要研究內(nèi)容如下： 1、我們制備了一種基于硅基納米材料的表面增強拉曼散射基質(zhì)，即在硅片上沉積了一層銀納米粒子（silver nanoparticles-decorated silicon wafers,AgNPs@Si），并將其用于進行高靈敏、特異性的DNA多元檢測。所制備AgNPs@Si具有極好的SERS信號重現(xiàn)性和較高的增強因子。我們的實驗結(jié)果進一步的證明了這種硅基SERS基底能夠有效地進行DNA的高靈敏、特異性多元檢測。該研究對于發(fā)展具有良好重現(xiàn)性的SERS基質(zhì)，發(fā)展基于SERS的生物分子檢測方法具有積極的意義。 2、我們采用3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(MTT)細胞毒性測試、熒光成像和流式細胞術(shù)等經(jīng)典生物學(xué)方法，，系統(tǒng)研究了三種不同表面性質(zhì)的硅納米線對腫瘤細胞系和正常細胞株在不同條件下（如硅納米線濃度、共孵育時間等）的細胞毒性。研究結(jié)果發(fā)現(xiàn)：細胞毒性對硅納米線濃度和共孵育時間具有明顯依賴性：隨著硅納米線濃度的增加和共孵育時間的延長，細胞活性呈明顯下降趨勢；在相同條件下，腫瘤細胞株系較正常細胞株系具有更高的細胞活性；硅納米線表面性質(zhì)對細胞活性具有明顯的影響。在此基礎(chǔ)上,我們給出了硅納米線用于細胞層面生物應(yīng)用的安全閾值。
[Abstract]:Silicon nanomaterials are important semiconductor nanomaterials. They have excellent electrical and mechanical properties, good biocompatibility, large specific surface area, easy surface modification, and compatibility with traditional silicon technology. Therefore, there is a strong interest in the development of functionalized silicon nanomaterials, which have been widely used in electronics and biology. In this paper, the surface enhanced Raman scattering (SERS) matrix based on silicon-based nanomaterials has been developed and used for high sensitivity and specific multivariate detection of surface-enhanced, and the biological effects of silicon nanowires at the cellular level have also been studied. The main research contents are as follows: 1. We have prepared a surface-enhanced Raman scattering substrate based on silicon-based nanomaterials, that is, we deposited a silver nanoparticles-decorated silicon wafers AgNPs @ Si on silicon wafers and used it for high sensitivity. Specific DNA multivariate detection. The prepared AgNPs @ Si has excellent SERS signal reproducibility and high enhancement factor. Our experimental results further demonstrate that the Si-based SERS substrate can be used to detect DNA with high sensitivity and specificity. This study has positive significance for the development of SERS substrates with good reproducibility and the development of SERS-based biomolecules detection methods. 2. We used classical biological methods, such as 3- (4t5) -dimethylthiahiazo (-z-y1) -3-di-phenytetrazoliumromide (MTT) cytotoxicity test, fluorescence imaging and flow cytometry, etc. The cytotoxicity of three kinds of silicon nanowires with different surface properties to tumor cell lines and normal cell lines under different conditions such as the concentration of silicon nanowires and co-incubation time were studied. The results showed that the cytotoxicity was significantly dependent on the concentration and co-incubation time of silicon nanowires: with the increase of the concentration of silicon nanowires and the prolongation of co-incubation time, the cell activity decreased obviously. The cell activity of tumor cell line was higher than that of normal cell line, and the surface property of silicon nanowire had obvious influence on cell activity. On this basis, we give the safety threshold of silicon nanowires for biological applications at cell level.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08

【相似文獻】

相關(guān)期刊論文 前10條

1 ;國外動態(tài)[J];納米科技;2011年02期

2 沈方云;李本義;;納米生物感應(yīng)器前列腺特異抗原檢測的研究進展[J];國際泌尿系統(tǒng)雜志;2006年05期

3 ;單生物芯片構(gòu)建單細胞病毒檢測系統(tǒng)[J];國外科技動態(tài);2004年11期

4 王連英,紀小會,袁航,馬嵐,孔祥貴,劉益春,楊文勝,白玉白,李鐵津;一種乙肝病毒表面抗原和其單克隆抗體相互作用的SERS研究[J];化學(xué)學(xué)報;2002年12期

5 沈星燦,袁琦,梁宏,閆海剛,何錫文;血清白蛋白與銀納米粒子相互作用的滯后效應(yīng)[J];中國科學(xué)B輯;2003年02期

6 ;印度科研人員稱銀納米粒子具有抗凝血功效[J];納米科技;2009年03期

7 蔣治良;陳媛媛;梁愛惠;陶慧林;唐寧莉;鐘福新;;痕量纖維蛋白原的銀納米標記免疫共振散射光譜分析[J];中國科學(xué)(B輯:化學(xué));2006年05期

8 蘇小東;賈云;李毅;;銀納米粒子探針等離子共振散射光譜法測定抗壞血酸[J];應(yīng)用化學(xué);2010年12期

9 呂曉迎;黃炎;馬靖武;;基于生物組學(xué)方法的銀納米粒子細胞毒性機理研究[J];口腔材料器械雜志;2010年04期

10 李靖云;王q

本文編號：2090301