本文選題：生物標(biāo)志物 + 多光譜成像技術(shù)��； 參考：《合肥工業(yè)大學(xué)》2017年碩士論文

【摘要】：生物標(biāo)志物是一類能夠反映人體疾病、自然環(huán)境等相關(guān)情況的信號指標(biāo),在疾病預(yù)判與診斷、藥物療效評估、環(huán)境暴露評估等領(lǐng)域具有廣泛應(yīng)用。頭發(fā)由于便于收集、運(yùn)輸方便、性質(zhì)穩(wěn)定、保存成本低、易于被接受等優(yōu)點(diǎn),已被廣泛認(rèn)可為評估疾病、環(huán)境、藥物和毒物的理想生物標(biāo)志物。頭發(fā)暴露于皮膚的最外層,相對于皮膚其更易受到紫外輻照,因此頭發(fā)可以作為紫外輻照評估的靶器官。紫外會(huì)對頭發(fā)造成損傷,從而頭發(fā)的光損傷程度可以用于評估人體接收的紫外輻照量�；诙喙庾V成像技術(shù),本文通過分段研究的方式對頭發(fā)的光損傷進(jìn)行定性分析,并通過電子掃描電鏡進(jìn)行表征。研究結(jié)果表明,生長時(shí)間越長的頭發(fā)具有更嚴(yán)重的光損傷程度,其平均光譜反射值在近紅外區(qū)域也顯著升高。在進(jìn)一步實(shí)驗(yàn)中,我們將同一段頭發(fā)在紫外燈下輻照特定時(shí)間并測定輻照前后的胱氨酸含量。研究結(jié)果表明,紫外輻照可以精確再現(xiàn)生長不同時(shí)間的頭發(fā)段的光譜反射值;且輻照后頭發(fā)的胱氨酸含量減小,表明胱氨酸含量的損失是導(dǎo)致頭發(fā)纖維表面形貌變化,從而導(dǎo)致頭發(fā)光譜反射值的變化的主要原因。在另一方面,頭發(fā)中的重金屬含量有助于評估人體重金屬暴露及體內(nèi)重金屬含量水平。本文基于多光譜成像技術(shù),研究了頭發(fā)纖維表面的重金屬累積。研究結(jié)果表明,頭發(fā)表面重金屬累積越多,在505 nm~700 nm處的光譜反射值越低;且頭發(fā)的損傷程度越嚴(yán)重,吸附重金屬離子的能力越強(qiáng)。本工作表明多光譜成像技術(shù)可以應(yīng)用于頭發(fā)光損傷和重金屬累積的快速、無損評估,為建立模型評估紫外輻照及重金屬累積程度提供了可行性,并且為后期通過多光譜成像技術(shù)檢測頭發(fā)以評估人體的重金屬含量、環(huán)境暴露、藥物和毒物暴露等提供了理論基礎(chǔ),對于促進(jìn)人體健康研究具有重要意義。
[Abstract]:Biomarker is a kind of signal index which can reflect the related situation of human body disease, natural environment and so on. It is widely used in the field of disease prediction and diagnosis, drug efficacy evaluation, environmental exposure assessment and so on. Hair has been widely recognized as an ideal biomarker for evaluating diseases, environment, drugs and poisons because of its advantages such as easy collection, convenient transportation, stable nature, low preservation cost and easy acceptance. Hair exposed to the outermost layer of the skin is more susceptible to UV irradiation than the skin, so hair can be used as a target organ for UV radiation evaluation. UV can damage the hair, so the degree of light damage can be used to evaluate the UV radiation received by the human body. Based on the multispectral imaging technique, the light damage of hair was analyzed qualitatively and characterized by electron scanning electron microscope (SEM). The results show that the longer the growth time, the more serious the light damage, and the higher the average spectral reflectance is in the near infrared region. In further experiments, the same hair was irradiated under ultraviolet lamp for a specific time and the cystine content was measured before and after irradiation. The results show that UV irradiation can accurately reproduce the spectral reflectance of hair growth at different time, and the cystine content of hair decreases after irradiation, which indicates that the loss of cystine content leads to the change of the surface morphology of hair fiber. As a result, the main reason for the change of the spectral reflectance of hair. On the other hand, the content of heavy metals in hair helps to assess heavy metal exposure and heavy metal levels in the body. The accumulation of heavy metals on the surface of hair fibers was studied based on multispectral imaging technique. The results show that the higher the accumulation of heavy metals on the hair surface, the lower the spectral reflectance at 505 nm~700 nm, and the more serious the damage is, the stronger the ability to adsorb heavy metal ions. This work shows that multispectral imaging can be applied to the rapid and nondestructive assessment of hair light damage and heavy metal accumulation, which provides a feasible model for the assessment of UV irradiation and heavy metal accumulation. It also provides a theoretical basis for the assessment of heavy metal content, environmental exposure, drug and poison exposure through multispectral imaging technology, which is of great significance to the study of human health.
【學(xué)位授予單位】：合肥工業(yè)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R312

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本文編號：2080996