本文選題：組織工程 + 軟骨��； 參考：《中國組織工程研究》2017年14期

【摘要】：背景:聚二甲基硅氧烷因具有良好的生物相容性,優(yōu)異的微納米尺度可加工性,被廣泛應(yīng)用于細(xì)胞生物學(xué)的基礎(chǔ)研究。但聚二甲基硅氧烷材料疏水性強,并不適于細(xì)胞貼附,因此表面修飾非常重要。目的:考察不同表面修飾方法并結(jié)合聚二甲基硅氧烷材料力學(xué)強度變化對牛關(guān)節(jié)軟骨細(xì)胞生物學(xué)行為的影響。方法:采用3種不同方法修飾聚二甲基硅氧烷薄膜材料,包括胎牛血清孵育、膠原沉積和等離子體處理,同時變化聚二甲基硅氧烷材料的硬度,然后接種牛關(guān)節(jié)軟骨細(xì)胞,并通過細(xì)胞骨架染色以及CCK-8表征分析其黏附、增殖情況,通過天狼星紅/番紅O染色以及糖胺聚糖定量表征分析基質(zhì)分泌情況。結(jié)果與結(jié)論:(1)細(xì)胞黏附和增殖:細(xì)胞骨架染色以及生長曲線可以看出血清孵育、膠原沉積和等離子體處理均能顯著改善牛關(guān)節(jié)軟骨細(xì)胞在聚二甲基硅氧烷表面的黏附和增殖,其中等離子體處理的效果最好;(2)糖胺聚糖的總量:只有等離子體處理的表面顯著提高了細(xì)胞分泌的糖胺聚糖的總量,但是細(xì)胞分泌糖胺聚糖的能力在所有修飾的表面上有所降低;(3)材料硬度對細(xì)胞黏附、生長和糖胺聚糖分泌都有影響,而且與材料表面修飾有關(guān)。(4)結(jié)果證實:實驗利用血清孵育、膠原沉積以及等離子體處理聚二甲基硅氧烷材料,均能有效促進(jìn)牛關(guān)節(jié)軟骨細(xì)胞的貼附和生長,而且其中等離子體處理效果最優(yōu),與此同時聚二甲基硅氧烷基材的硬度也對細(xì)胞行為有所影響,但程度上要次于化學(xué)修飾,而且越能促進(jìn)細(xì)胞生長的材料越抑制了關(guān)節(jié)軟骨細(xì)胞的基質(zhì)分泌能力。
[Abstract]:Background: Poly (dimethylsiloxane) has been widely used in the basic research of cell biology because of its good biocompatibility and excellent micro- and nanoscale processability. However, polydimethylsiloxane is not suitable for cell adhesion because of its strong hydrophobicity, so surface modification is very important. Aim: to investigate the effects of different surface modification methods and mechanical strength of polydimethylsiloxane on the biological behavior of bovine articular chondrocytes. Methods: polydimethylsiloxane membrane materials were modified by three different methods, including fetal bovine serum incubation, collagen deposition and plasma treatment, and the hardness of polydimethylsiloxane materials was changed at the same time, and then bovine articular chondrocytes were inoculated. The adhesion and proliferation were analyzed by cytoskeleton staining and CCK-8 staining. The matrix secretion was analyzed by Sirius red / vanillin O staining and glycosaminoglycan quantitative analysis. Results and conclusion: (1) Cell adhesion and proliferation: cytoskeleton staining and growth curve showed that serum incubation, collagen deposition and plasma treatment could significantly improve the adhesion and proliferation of bovine articular chondrocytes on the surface of polydimethylsiloxane. The effect of plasma treatment was the best. (2) the total amount of glycosaminoglycan: only the surface treated by plasma significantly increased the total amount of glycosaminoglycan secreted by cells. However, the ability of cells to secrete glycosaminoglycan was decreased on all the modified surfaces. (3) the hardness of the material affected cell adhesion, growth and glycosaminoglycan secretion, and was related to the surface modification of the material. (4) the results showed that the experiment was incubated with serum. Collagen deposition and plasma treatment of polydimethylsiloxane can effectively promote the adhesion and growth of bovine articular chondrocytes, and the plasma treatment is the best. At the same time, the hardness of polydimethylsiloxane also affected the cell behavior, but to a lesser extent than the chemical modification, and the more the material can promote cell growth, the more inhibit the matrix secretion ability of articular chondrocytes.
【作者單位】： 華東理工大學(xué)生物反應(yīng)器工程國家重點實驗室;
【基金】：上海市自然科學(xué)基金項目(16ZR1408700)~~
【分類號】：R318.08;R68
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本文編號：2079551