本文選題：周期性流體靜壓力 + 骨髓間充質(zhì)干細(xì)胞　； 參考：《口腔醫(yī)學(xué)研究》2017年06期

【摘要】：目的:觀察周期性流體靜壓力作用下骨髓間充質(zhì)干細(xì)胞(Bone Marrow Stem cells,BMSCs)細(xì)胞增殖活性及超微結(jié)構(gòu)的改變。方法:貼壁法分離培養(yǎng)大鼠BMSCs。采用自主研發(fā)的流體靜壓力加載裝置對大鼠BMSCs進(jìn)行力學(xué)加載,加載壓力為0~45kPa、0~90kPa,加載頻率為0.1 Hz,時間1h,連續(xù)加載2d。對照組細(xì)胞不加壓。采用CCK8法檢測細(xì)胞的增殖活性,采用透射電鏡觀察大鼠BMSCs超微結(jié)構(gòu)的變化。結(jié)果:0~45kPa、0~90kPa周期性流體靜壓力作用下BMSCs增殖活性顯著高于對照組(P0.05),透射電鏡下觀察發(fā)現(xiàn),0~45kPa作用下BMSCs細(xì)胞內(nèi)質(zhì)網(wǎng)輕度擴(kuò)張,膠原合成量增加。而0~90kPa作用下細(xì)胞出現(xiàn)了大量膠原及凋亡小體。結(jié)論:適宜的周期性流體靜壓力作用下可提高細(xì)胞的增殖活性,但過高的壓力刺激可誘導(dǎo)細(xì)胞產(chǎn)生凋亡。
[Abstract]:Aim: to observe the proliferative activity and ultrastructure of bone marrow stem cells (BMSCs) under cyclic hydrostatic pressure. Methods: BMSCs were isolated and cultured by adherent method. The BMSCs were subjected to mechanical loading with a hydrostatic pressure loading device developed by ourselves. The loading pressure was 0.45kPa0.90kPa0.The loading frequency was 0.1 Hz for 1 hour, and the loading time was 2 days. The cells in the control group were not pressurized. The proliferation activity of BMSCs was detected by CCK8 method and the ultrastructure of BMSCs was observed by transmission electron microscope. Results the proliferative activity of BMSCs under 90 KPA periodic hydrostatic pressure was significantly higher than that of control group P0.05. the endoplasmic reticulum of BMSCs was slightly dilated and collagen synthesis increased under transmission electron microscope. However, a large number of collagen and apoptotic corpuscles appeared in the cells treated with 0 ~ 90 KPA. Conclusion: the cell proliferation activity can be increased under the action of periodic hydrostatic pressure, but the cell apoptosis can be induced by excessive pressure stimulation.
【作者單位】： 解放軍第518醫(yī)院口腔中心;軍事口腔醫(yī)學(xué)國家重點(diǎn)實(shí)驗(yàn)室國家口腔疾病臨床醫(yī)學(xué)研究中心陜西省口腔疾病國際聯(lián)合研究中心第四軍醫(yī)大學(xué)急診與綜合臨床科;
【基金】：國家自然科學(xué)基金(編號:81371188;31570951)
【分類號】：R318.08
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本文編號：2048656