發(fā)布時間：2018-06-18 13:00

  本文選題：透明質酸鈉 + 魔芋葡甘聚糖　； 參考：《昆明理工大學》2012年碩士論文

【摘要】：硬膜外粘連的形成被認為是脊柱手術后疼痛的核心因素,并且被認為是壓迫神經(jīng)的最主要原因,而防粘連材料的應用是預防粘連一種有效的方式。一定濃度的透明質酸鈉(Sodium hyaluronate,簡稱SH)可抑制成纖維細胞增殖和運動,在防粘連部位,具有抑制出血、消炎的功效,因此是一種理想的防粘連材料,但其凝膠在體內降解率快,僅在粘連早期有一定的防粘連功效。因此,本課題采用多糖與SH復合制備成共混膜,以期待能夠減緩材料的降解,從而長期預防粘連。 1、本論文首先通過透明質酸鈉、魔芋葡甘聚糖(Konjac glucomannan,簡稱KGM)為原料,乳酸作為添加劑,與微波作用共同降低膜液粘度,采用氨水／無水乙醇的混合溶液作為交聯(lián)劑,制備不可水溶的SH/KGM共混膜；通過正交試驗方案L16(45)對SH/KGM共混膜的制備工藝進行了研究。 檢測結果顯示：該共混膜的膜液粘度在290～6267mPa·s,膜厚在41.8-54.5μm之間,拉伸強度與斷裂伸長率分別在11.8-17.4MPa、7.1-20.8%之間,吸水率分布151-508%之間,而10周共混膜的降解率在27.1-43.3%之間；并通過對以上結果的綜合評定,確定最優(yōu)方案為魔芋粉2.75g、SH0.09g、乳酸0.9ml、微波加熱時間4min、交聯(lián)時間為4d。 該最優(yōu)組復合膜通過體外細胞培養(yǎng)及兔子體內皮下包埋實驗發(fā)現(xiàn)SH/KGM共混膜與細胞的粘附性能較弱,但該膜在兔子體內并未導致膿腫、組織變性、壞死和異常增生,因此說明該膜具有一定的生物相容性；通過體外降解實驗及體內包埋實驗發(fā)現(xiàn)該膜的降解率不高,在PBS溶液中的體外降解率35.8%,與純SH凝膠相比,其降解率大幅度減緩；該復合膜具有較高的吸水率,吸濕后帖附性能好,且吸水后有一定的韌性,可保證在使用過程中不脆斷。 2、本論文接著研究了透明質酸鈉／殼聚糖(Chitosan,簡稱CS)／魔芋葡甘聚糖共混膜,以透明質酸鈉、殼聚糖為主要原料,KGM作為改性劑,乙酸為添加劑,經(jīng)充分溶解、減壓脫泡、流延成膜、烘干及交聯(lián)后制備不可溶性共混膜,并判斷KGM含量對共混膜性質的影響。 SH/CS/KGM復合膜呈淡黃色,一面粗糙,一面較為光滑；從透光率和掃描電鏡圖發(fā)現(xiàn),該共混膜具有較好的共混相容性；經(jīng)研究發(fā)現(xiàn)該共混膜具有良好的生物相容性,可支持細胞在膜上生長,且細胞具有良好的形態(tài)；但該復合膜的降解率較低,10周的體外降解率僅在5.78%-6.47%。 KGM的加入提高了共混膜的韌性(斷裂伸長率在7.1-20.8%),解決了透明質酸鈉/殼聚糖共混膜韌性不足的缺點；且提高了共混膜的吸水率,較高的吸水率可吸附損傷部位的組織積液,起到止血的作用,減少瘢痕的形成,從而減輕炎癥；但KGM的加入使得共混膜的生物相容性略有降低。
[Abstract]:The formation of epidural adhesion is considered to be the core factor of pain after spinal surgery and the main cause of nerve compression. The application of anti-adhesion materials is an effective way to prevent adhesion. Sodium hyaluronate at a certain concentration can inhibit the proliferation and movement of fibroblasts. In the anti-adhesion site, it has the effect of inhibiting bleeding and anti-inflammation, so it is an ideal anti-adhesion material, but its gel degrades rapidly in vivo. Only in the early stage of adhesion has a certain anti-adhesion effect. Therefore, a blend membrane was prepared with polysaccharide and SH in order to slow down the degradation of the material and prevent adhesion for a long time. 1. In this paper, first of all, through sodium hyaluronate, Konjac glucomannan (KGM), Konjac glucomannan (KGM) was used as the raw material. Lactic acid was used as additive and microwave to reduce the viscosity of membrane liquid. The insoluble SH- / KGM blend membrane was prepared by using mixed solution of ammonia and anhydrous ethanol as crosslinking agent. The preparation process of SH- / KGM blend membrane was studied by orthogonal test scheme L _ (16) ~ (45). The results showed that the viscosity of the blend film was 290V 6267mPa / s, the film thickness was 41.8-54.5 渭 m, the tensile strength and elongation at break were between 11.8-17.4 MPA 7.1-20.8%, the water absorption distribution was 151-508%, and the degradation rate of the blend film was 27.1-43.3% at 10 weeks. Through the comprehensive evaluation of the above results, the optimal scheme was determined as follows: konjac powder 2.75 g / h 0.09 g, lactic acid 0.9 ml, microwave heating time 4 min, crosslinking time 4 d. The results of cell culture in vitro and subcutaneous embedding in rabbits showed that the adhesion of SH- KGM blend membrane to cells was weak, but the membrane did not cause abscess, tissue degeneration, necrosis and abnormal proliferation in rabbits. It was found that the degradation rate of the film was not high in vitro and in vivo, and the degradation rate in PBS solution was 35.8%. Compared with pure SH gel, the degradation rate of the film was much slower than that of pure SH gel. The composite film has high water absorption, good moisture absorption property, and good toughness after water absorption. 2. In this paper, sodium hyaluronate / chitosan Chitosan (CSN / Konjac glucomannan) blend membrane was studied. Sodium hyaluronate and chitosan were used as the main raw materials, and KGM was used as modifier. Acetic acid was used as additive to prepare insoluble blend membrane after fully dissolving, defoaming under pressure, forming film by casting, drying and crosslinking, and the effect of KGM content on properties of blend membrane was evaluated. SHR / CS / KGM composite membrane was yellowish and rough. It was found that the blend film had good compatibility and biocompatibility, which could support the growth of cells on the membrane, and the cells had good morphology, and the blend film had a good compatibility from the light transmittance and scanning electron microscope (SEM), and the study showed that the blend film had a good biocompatibility and the morphology of the cells was good. However, the degradation rate of the composite membrane in vitro was only 5.78-6.47 after 10 weeks of low degradation. The addition of KGM improved the toughness of the blend film (elongation at break was 7.1-20.8%), which solved the disadvantage of insufficient toughness of sodium hyaluronate / chitosan blend film. Moreover, the water absorption rate of the blend membrane was increased, and the higher water absorption rate could adsorb the tissue effusion in the damaged area, which could stop bleeding and reduce the formation of scar, thus reducing inflammation, but the biocompatibility of the blend membrane was slightly decreased with the addition of KGM.
【學位授予單位】：昆明理工大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：TB383.2;R318.08

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