本文選題：鍶 + 透鈣磷石�。� 參考：《中國(guó)人民解放軍醫(yī)學(xué)院》2014年博士論文

【摘要】：研究背景 種植體的表面形貌和成分影響種植體的骨結(jié)合及種植體長(zhǎng)期存活率。純鈦種植體沒(méi)有誘導(dǎo)成骨的效用，HA涂層不易吸收，有脫殼現(xiàn)象。透鈣磷石具有良好的生物相容性，在生理?xiàng)l件下具有較快的降解速率。同時(shí)低劑量的鍶具有促進(jìn)骨形成，抑制骨吸收的雙重作用。本研究應(yīng)用電化學(xué)沉積法制備了鈦基摻鍶透鈣磷石涂層。希望鍶和透鈣磷石協(xié)同作用，提高種植體骨結(jié)合效率，為擴(kuò)大種植義齒適應(yīng)癥、提高牙種植體成功率提供新的思路。 材料和方法 1應(yīng)用電化學(xué)沉積法在純鈦板表面制備鈦基摻鍶透鈣磷石涂層。鍶的濃度為0，5%，10%，和20%。應(yīng)用X射線能譜和X-射線衍射對(duì)涂層進(jìn)行表征分析。通過(guò)掃描電子顯微鏡觀察涂層的表面形貌。 2應(yīng)用電化學(xué)沉積法在純鈦種植體表面制備摻鍶透鈣磷石涂層。將種植體植入SD大鼠脛骨近心端。應(yīng)用硬組織切片技術(shù)和Micro-CT觀察摻鍶鈣磷涂層種植體的骨結(jié)合率。 3提取不同比例摻鍶透鈣磷石涂層的浸提液，在浸提液中培養(yǎng)MC3T3-E1成骨細(xì)胞，觀察細(xì)胞生長(zhǎng)情況，應(yīng)用MTT檢測(cè)細(xì)胞毒性和增殖，ALP檢測(cè)細(xì)胞活性，并應(yīng)用RT-PCR檢測(cè)bFGF和VEGF的mRNA表達(dá)情況。 結(jié)果 1通過(guò)電沉積法在鈦基表面制備出均勻的摻鍶透鈣磷石涂層，涂層主要由不同形態(tài)的晶體組成，主要物相為磷酸氫鍶和透鈣磷石； 2通過(guò)調(diào)整不同的鍶和鈣摩爾比，可得到不同含鍶濃度的摻鍶透鈣磷石涂層。 3鍶與透鈣磷石協(xié)同促進(jìn)了種植體骨結(jié)合； 4摻鍶透鈣磷石涂層提高了種植體骨結(jié)合率；10%摻鍶透鈣磷石涂層效果顯著。 5摻鍶透鈣磷石涂層促進(jìn)了成骨細(xì)胞ALP的表達(dá)； 6摻鍶透鈣磷石涂層具有較好的安全性和生物相容性； 7摻鍶透鈣磷石涂層上調(diào)了成骨細(xì)胞bFGF及VEGF mRNA的表達(dá)水平，從而明顯增強(qiáng)其對(duì)成骨細(xì)胞分化的誘導(dǎo)作用； 810%摻鍶透鈣磷石涂層具有較顯著的促進(jìn)成骨細(xì)胞生長(zhǎng)的作用。 結(jié)論： 電化學(xué)沉積及體內(nèi)外實(shí)驗(yàn)結(jié)果表明，電化學(xué)沉積摻鍶透鈣磷石涂層可以促進(jìn)成骨細(xì)胞生長(zhǎng)，促進(jìn)種植體的骨結(jié)合。為摻鍶透鈣磷石涂層種植體的臨床應(yīng)用奠定了理論基礎(chǔ)，具有一定的的臨床應(yīng)用價(jià)值。
[Abstract]:Background the surface morphology and composition of implants affect bone bonding and long-term survival of implants. Pure titanium implants have no osteogenic effect. Ha coating is not easy to be absorbed and can be dehulled. The permeable phosphorite has good biocompatibility and fast degradation rate under physiological conditions. At the same time, low dose strontium has dual effects of promoting bone formation and inhibiting bone resorption. In this paper, titanium-doped strontium permeable calcium phosphate coating was prepared by electrochemical deposition. It is hoped that the synergistic effect of strontium and pericalciferite could improve the efficiency of implant bone bonding and provide a new idea for expanding the indication of implant denture and improving the success rate of dental implant. Materials and methods 1 Titanium doped strontium permeable calcium phosphate coating was prepared on the surface of pure titanium plate by electrochemical deposition. The concentration of strontium is 0, 5, 10, and 20. The coating was characterized by X ray energy spectrum and X ray diffraction. The surface morphology of the coating was observed by scanning electron microscope. 2 Strontium permeable calcium phosphate coating was prepared on the surface of pure titanium implant by electrochemical deposition. Implants were implanted into the proximal heart of the tibia of SD rats. The bone binding rate of strontium doped calcium phosphate coated implants was observed by hard tissue section technique and Micro-CT. 3 extraction solution with different proportion of strontium doped calcium phosphate coating was extracted, MC3T3-E1 osteoblasts were cultured in the extract, and cell growth was observed. The cytotoxicity and proliferation of ALP were detected by MTT, and the mRNA expressions of bFGF and VEGF were detected by RT-PCR. Results (1) Strontium permeable calcium phosphate coating was prepared on titanium substrate by electrodeposition. The coating mainly consisted of crystals of different morphology, and the main phases were strontium hydrogen phosphate and calcium permeable phosphate. (2) by adjusting the molar ratio of strontium and calcium, strontium permeable phosphor coating with different strontium concentration can be obtained. (4) Strontium permeable calcium phosphate coating improved the bone bonding rate of implant and the effect of 10% strontium permeable calcium phosphate coating was significant. 5 the ALP expression of osteoblasts was promoted by strontium permeable calcium phosphate coating. 6Strontium permeable calcium phosphate coating has good safety and biocompatibility, 7 strontium permeable calcium phosphate coating up-regulates the expression of bFGF and VEGF mRNA in osteoblasts. Thus the induction of osteoblast differentiation was obviously enhanced and 810% strontium permeable calcium phosphate coating could significantly promote the growth of osteoblasts. Conclusion: the results of electrochemical deposition and in vitro and in vivo experiments showed that Sr-doped calcium phosphate coating could promote the growth of osteoblasts and promote the bone bonding of implants. It lays a theoretical foundation for the clinical application of strontium permeable calcium phosphate coated implants and has certain clinical application value.
【學(xué)位授予單位】：中國(guó)人民解放軍醫(yī)學(xué)院
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2014
【分類號(hào)】：R783.1

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