本文選題：鈦合金 + 酸堿處理��； 參考：《蘭州大學(xué)》2012年碩士論文

【摘要】：鈦合金具有理想的理化性能和良好的生物相容性,是臨床常用的人工植入材料,由于其本身沒有骨誘導(dǎo)活性,與周圍骨組織不能形成足夠的化學(xué)結(jié)合,使臨床應(yīng)用受限。羥基磷灰石(Hydroxyapatite, HA)與骨組織的化學(xué)成分和結(jié)構(gòu)類似,可通過化學(xué)鍵與骨組織結(jié)合并誘導(dǎo)新骨生成。因此,采用羥基磷灰石涂層方法已成為鈦合金表面常用的改性手段之一,但是涂層與基底結(jié)合存在一定問題,影響了植入體長期的穩(wěn)定性,有必要探討新的方法對鈦合金表面進行改性。 本論文采用兩種化學(xué)方法：酸堿結(jié)合法和多巴胺自組裝技術(shù)對鈦合金表面進行處理形成過渡層,通過模擬體液(SBF)仿生礦化制備羥基磷灰石活性涂層。利用掃描電子顯微鏡(SEM)、X射線衍射儀(XRD)、光學(xué)接觸角測量儀、傅里葉變換紅外光譜儀(FTIR)、X-射線光電子能譜儀(XPS)等分析測試手段對改性前、后鈦合金表面的形貌、物相結(jié)構(gòu)、潤濕性以及組成成分等進行了分析表征。同時,采用材料與成骨細胞體外共培養(yǎng)的方式,研究了兩種方法誘導(dǎo)生成的HA活性涂層的細胞相容性。 主要研究結(jié)果如下： 1)通過對不同酸溶液體系及不同濃度的堿液處理鈦合金表面的方法進行對比,觀察到不同溶液處理后的鈦合金表面腐蝕狀況不同,單一的處理不如酸堿聯(lián)合處理得到的網(wǎng)孔狀結(jié)構(gòu)均勻。借助SEM、XRD和FTIR等分析,發(fā)現(xiàn)酸堿結(jié)合處理后仿生礦化形成了成分單一的HA涂層。細胞實驗表明成骨細胞早期粘附數(shù)量及增殖率高于未處理的對照組,顯示了良好的細胞相容性。 2)通過分子自組裝技術(shù)在鈦合金表面自組裝聚多巴胺形成過渡層,經(jīng)生物礦化形成HA活性涂層,通過SEM、XRD與FTIR表征證實所得涂層為含碳的羥基磷灰石,且排列比較致密。細胞實驗也證實了該種方法得到的HA涂層表現(xiàn)出了優(yōu)異的細胞粘附與增殖能力,有望成為鈦合金表面活化處理的一種新的方法,具有良好的臨床應(yīng)用前景。
[Abstract]:Titanium alloy has ideal physical and chemical properties and good biocompatibility. It is a commonly used artificial implant material in clinic. Because of its lack of bone inductive activity, it can not form enough chemical combination with surrounding bone tissue, so its clinical application is limited. Hydroxyapatite (HA), which is similar to the chemical composition and structure of bone tissue, can bind to bone tissue and induce new bone formation by chemical bond. Therefore, hydroxyapatite coating method has become one of the commonly used modification methods on titanium alloy surface, but there are some problems in the combination of coating and substrate, which affect the long-term stability of the implant. It is necessary to explore a new method for surface modification of titanium alloy. In this paper, two chemical methods, acid-base binding method and dopamine self-assembly technique, were used to treat titanium alloy surface to form transition layer. Hydroxyapatite active coating was prepared by simulated SBF biomimetic mineralization. The surface morphology and phase structure of titanium alloy before and after modification were analyzed by means of scanning electron microscope (SEM), SEMU X-ray diffractometer, optical contact angle measuring instrument, Fourier transform infrared spectrometer (FTIR) and X-ray photoelectron spectroscopy (XPS). The wettability and composition were analyzed and characterized. At the same time, the cytocompatibility of HA active coatings induced by two methods was studied by co-culture of materials and osteoblasts in vitro. The main findings are as follows: 1) by comparing the methods of treating titanium alloy surface with different acid solution system and different concentration of alkali solution, it is observed that the corrosion condition of titanium alloy treated with different solution is different. The single treatment is not as uniform as the mesh structure obtained by acid-base combined treatment. By means of FTIR and FTIR analysis, it was found that the HA coating with single composition was formed by biomimetic mineralization after acid-base treatment. Cell experiments showed that the number and proliferation rate of osteoblasts in the early stage were higher than those in the untreated control group and showed good cytocompatibility. 2) Self-assembled polydopamine transition layer was formed on the surface of titanium alloy by molecular self-assembly technique. HA active coating was formed by biomineralization. The coating was characterized by FTIR and FTIR, and the coating was carbon containing hydroxyapatite with compact arrangement. The cell experiments also confirmed that the HA coating obtained by this method showed excellent cell adhesion and proliferation, which is expected to be a new method for surface activation treatment of titanium alloy, and has a good prospect of clinical application.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08

【參考文獻】

相關(guān)期刊論文 前10條

1 黃偉九;李兆峰;;醫(yī)用鈦合金表面改性研究進展[J];材料導(dǎo)報;2006年S2期

2 寧成云;王迎軍;陳曉峰;趙娜如;;梯度結(jié)構(gòu)羥基磷灰石生物活性涂層的性能[J];材料研究學(xué)報;2006年01期

3 謝檳;李貴才;代璐;楊蘋;黃楠;;基于多巴胺自聚合及肝素固定改善鈦的血液相容性[J];高等學(xué)�；瘜W(xué)學(xué)報;2012年01期

4 肖琳琳;魏雨;計劍;;基于聚多巴胺輔助自組裝單分子層技術(shù)的PTFE表面修飾及其內(nèi)皮細胞選擇性黏附研究[J];高分子學(xué)報;2010年04期

5 林英光;楊卓如;程江;;納米摻鋅羥基磷灰石的制備及抗菌性能[J];華南理工大學(xué)學(xué)報(自然科學(xué)版);2007年07期

6 張玉瑋;張云;王樺;沈國勵;俞汝勤;;基于仿生聚多巴胺膜和納米金的酶固定化平臺的構(gòu)建[J];化學(xué)學(xué)報;2009年20期

7 王力平,曹陽,張波,包崇云,楊幫成,陳繼鏞,張興棟;醫(yī)用鈦表面組織結(jié)構(gòu)和形貌特征與表面生物活性的研究[J];化學(xué)研究與應(yīng)用;2004年03期

8 呂宇鵬,李士同,梁棟科,朱瑞富,李木森,雷廷權(quán);等離子體熔融鈦與羥基磷灰石混合粉顆粒的形態(tài)和相組成[J];金屬學(xué)報;2002年12期

9 謝永林;陳一;孫勇;;鈦及鈦合金種植體表面涂層處理的研究進展[J];川北醫(yī)學(xué)院學(xué)報;2011年01期

10 張高會,張平則,崔彩娥,郭麗娜,潘俊德,徐重;鈦合金及其表面處理的現(xiàn)狀與展望[J];世界科技研究與發(fā)展;2003年04期

，

本文編號：1985215