本文選題：醫(yī)用藻酸鹽敷料 + 體外降解��； 參考：《科學技術(shù)與工程》2016年03期

【摘要】：研究藻酸鹽醫(yī)用敷料體外降解的規(guī)律及不同降解時間其產(chǎn)物對內(nèi)皮細胞增殖情況的影響,為藻酸鹽醫(yī)用敷料在臨床使用中提供參考。以PBS緩沖液(模擬體液環(huán)境)為降解介質(zhì),對藻酸鹽敷料進行體外降解;用藻酸鹽敷料的失重率、分子質(zhì)量變化表征本體的變化規(guī)律;用降解液中降解產(chǎn)物的糖醛酸含量變化表征降解產(chǎn)物的變化規(guī)律。將降解液與內(nèi)皮細胞共培養(yǎng),用MTT法檢測內(nèi)皮細胞增殖情況。藻酸鹽敷料在模擬體液環(huán)境條件下,可以被降解,42 d失重率為61.2%,降解過程中其分子質(zhì)量逐漸減小,最終產(chǎn)物為糖醛酸,其濃度不斷增大。不同時間的降解產(chǎn)物均能促進內(nèi)皮細胞的增殖。藻酸鹽醫(yī)用敷料體外降解比較快,完全降解的最終產(chǎn)物為糖醛酸,其降解液可以促進內(nèi)皮細胞的增殖、分化,提高細胞新陳代謝能力,增加新細胞的再生速度,促進傷口的愈合。
[Abstract]:To study the rule of alginate medical dressing degradation in vitro and the effect of different degradation time on the proliferation of endothelial cells, and to provide reference for alginate medical dressing in clinical use. The alginate dressing was degraded in vitro with PBS buffer (simulated humoral environment) as the degradation medium, the weight loss rate and molecular weight change of alginate dressing were used to characterize the change of body mass. The change rule of degradation product was characterized by the change of uronic acid content in degradation solution. The degradation solution was co-cultured with endothelial cells and the proliferation of endothelial cells was detected by MTT assay. Under the simulated humoral environment, the weight loss rate of alginate dressing can be reduced to 61.2 for 42 days. The molecular weight of alginate dressing decreases gradually during the degradation process, and the final product is uronic acid, and the concentration of alginate dressing is increasing. The degradation products at different time could promote the proliferation of endothelial cells. Alginate medical dressing degrades rapidly in vitro, and the final product of complete degradation is uronic acid, which can promote the proliferation and differentiation of endothelial cells, improve cell metabolism and increase the regeneration rate of new cells. Promote wound healing.
【作者單位】： 廣東泰寶醫(yī)療科技股份有限公司;
【分類號】：R318.08

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