發(fā)布時(shí)間：2018-06-02 20:23

  本文選題：醫(yī)用生物膜 + 生物性能檢測(cè)��； 參考：《青島大學(xué)》2014年碩士論文

【摘要】：目的：制備新型醫(yī)用生物膜材料,研究制作方法,評(píng)價(jià)其生物相容性,為臨床做應(yīng)用準(zhǔn)備。 方法：無菌條件下取出隨機(jī)挑選健康豬的心包,通過胰酶去垢劑、凍融核酸酶兩種方法進(jìn)行脫細(xì)胞處理,制備出豬心包衍生醫(yī)用生物膜。通過光鏡觀察,與對(duì)照組對(duì)比,選出脫細(xì)胞良好的豬心包衍生醫(yī)用生物膜,利用大鼠皮下包埋實(shí)驗(yàn)對(duì)其進(jìn)行生物相容性檢測(cè)及吸收時(shí)間觀察。 結(jié)果：(1)胰酶去垢劑法和凍融核酸酶法兩組豬心包衍生醫(yī)用生物膜肉眼觀察顏色泛白,膜完整且光滑,質(zhì)地與新鮮豬心包相比無明顯變化。 (2)豬心包衍生醫(yī)用生物膜光鏡下觀察可見構(gòu)成清晰。漿膜層某些區(qū)域能看到細(xì)胞殘留,大部分已脫落。纖維層可見波浪狀排列的膠原纖維束。排列無明顯紊亂,無溶解現(xiàn)象。光鏡下均可見自身細(xì)胞基本脫除,新鮮對(duì)照組仍有大量固有細(xì)胞殘留。 (3)大鼠術(shù)后未應(yīng)用抗生素,未感染。切口自然愈合,無紅腫及異物排出。大鼠未出現(xiàn)局部、全身系統(tǒng)及行為異常,未出現(xiàn)病危及死亡。 (4)大鼠植入胰酶去垢法獲得的豬心包衍生醫(yī)用生物膜皮下包埋后,一周時(shí)沒明顯變化,二周后開始纖維斷裂,四周后淋巴細(xì)胞侵入,八周后標(biāo)本白細(xì)胞大量浸潤(rùn)。 結(jié)論：(1)胰酶去垢法處理的豬心包衍生醫(yī)用膜更符合實(shí)驗(yàn)要求。 (2)豬心包衍生應(yīng)用生物膜取材容易、制取方便、價(jià)格低廉,有良好的生物相容性,是較理想的可吸收膜替代材料。
[Abstract]:Objective: to prepare a new medical biofilm material, to evaluate its biocompatibility and to prepare for clinical application. Methods: the pericardium of healthy pigs was randomly selected under aseptic condition. The biofilm derived from porcine pericardium was prepared by means of trypsin descaling agent and freeze-thawing nuclease. The biomembrane derived from porcine pericardium was selected by light microscope and compared with the control group. The biocompatibility and absorption time of the biomembrane were detected by rat subcutaneous embedding test. Results 1) the piglet pericarpium derived medical biofilm was white in color, intact and smooth, and had no obvious change in texture compared with fresh porcine pericardium by trypsin defouling method and freeze-thawing nuclease method. 2) the structure of pig pericardium derived medical biofilm can be seen clearly under light microscope. Some areas of the serous layer can see cell residues, most of which have fallen off. A wavy collagenous bundle can be seen in the fibrous layer. There was no obvious disorder and no dissolution in the arrangement. Autologous cells were basically removed under light microscope, and a large number of inherent cells remained in the fresh control group. The rats were not treated with antibiotics and were not infected. The incision healed naturally without redness and foreign body excretion. There were no local, systemic and behavioral abnormalities, and no death and illness. 4) after subcutaneous embedding of porcine pericardial derived medical biofilm obtained by trypsin removing scale method, there was no obvious change at one week, the fibers began to break after two weeks, lymphocytes invaded after four weeks, and a large number of white blood cells were infiltrated in the specimen after eight weeks. Conclusion 1) the porcine pericardial derived medical membrane treated by trypsin decontamination is more suitable for the experiment. 2) the biofilm used in porcine pericarp derivation is an ideal substitute for absorbable membrane because it is easy to obtain, cheap, and has good biocompatibility.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R318.08

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