發(fā)布時(shí)間：2018-06-02 08:22

  本文選題：殼聚糖 + 微圖形�。� 參考：《西南交通大學(xué)》2012年碩士論文

【摘要】：殼聚糖(CS)是一種天然醫(yī)用生物材料。由于CS良好的成膜性、生物相容性以及優(yōu)異的抗菌性能,是藥物的良好載體,可作為藥物控釋膜。此外,由于殼聚糖可促進(jìn)造骨細(xì)胞成長(zhǎng)和骨質(zhì)沉積,可作為骨組織修復(fù)材料。 本研究通過(guò)微轉(zhuǎn)移模塑法,在硅片表面制得了不同形貌的殼聚糖微圖形(圓形點(diǎn)陣、方形點(diǎn)陣、溝槽和波浪)。制備的CS微圖形清晰規(guī)整,成型效果良好。成骨細(xì)胞在圓形點(diǎn)陣、方形點(diǎn)陣上借助偽足多攀附于點(diǎn)陣凸起。點(diǎn)陣圖形對(duì)細(xì)胞增殖、分化無(wú)影響。在溝槽和波浪中成骨細(xì)胞多被限制在溝區(qū),且沿著溝槽方向伸展。溝槽和波浪微圖形不利于細(xì)胞分化,但能促進(jìn)細(xì)胞增殖。 在功能化的鈦表面制備了殼聚糖和骨形態(tài)發(fā)生蛋白BMP-2的復(fù)合涂層,在保持BMP-2活性的基礎(chǔ)上,實(shí)現(xiàn)了BMP-2的緩慢釋放。鈦片表面功能化主要有三個(gè)步驟：堿處理、用APTES硅烷化以及用戊二醛醛基化。然后,通過(guò)戊二醛的醛基和CS的氨基間的反應(yīng)使得包埋有BMP-2的CS涂層固定于鈦片表面(BMP2-CS-Ti)。同時(shí),我們還通過(guò)在醛基化之后的鈦片表面直接固定BMP-2(BMP2-Ti)或CS(CS-Ti)作為對(duì)照樣。BMP2-CS-Ti表面BMP-2的釋放速度是BMP2-Ti的一半,證明CS涂層是良好的BMP-2載體,實(shí)現(xiàn)了BMP-2的穩(wěn)定釋放。通過(guò)體外培養(yǎng)骨髓間充質(zhì)干細(xì)胞(BMSCs),檢測(cè)BMP2-CS-Ti、BMP2-Ti、CS-Ti和pristine-Ti的骨誘導(dǎo)能力。結(jié)果表明BMP2-CS-Ti較其他三組更能促進(jìn)細(xì)胞分化。說(shuō)明CS/BMP-2共涂層穩(wěn)定,保持了BMP-2的活性,具有良好的骨誘導(dǎo)能力�？咕鷮�(shí)驗(yàn)證明CS/BMP-2共涂層對(duì)大腸桿菌和白色葡萄球菌都有良好的抗菌效果。
[Abstract]:Chitosan (CS) is a natural medical biomaterial. Due to its good film formation, biocompatibility and excellent antibacterial properties, CS is a good carrier of drugs and can be used as a drug controlled-release membrane. In addition, as chitosan can promote osteoblast growth and bone deposition, it can be used as a bone tissue repair material.
In this study, by microtransfer molding, different morphology of chitosan micrographs (circular lattice, square lattice, groove and wave) were prepared on the surface of the silicon wafer. The prepared CS micrograph is clear and regular and has good shaping effect. The osteoblasts are in a circular lattice and a square lattice is attached to the lattice bulge with the help of pseudofoot. The osteoblasts in the grooves and waves are mostly confined to the gully area, and extend along the groove direction. The grooves and wave micro patterns are not conducive to cell differentiation, but can promote cell proliferation.
The composite coating of chitosan and bone morphogenetic protein BMP-2 was prepared on the functional titanium surface, and the slow release of BMP-2 was realized on the basis of maintaining the activity of BMP-2. The surface functionalization of titanium sheet was mainly three steps: alkali treatment, APTES silanation and glutaraldehyde based aldehyde. And then, through the aldehyde group of glutaraldehyde and the amino group of CS. The reaction made the embedded CS coating embedded with BMP-2 on the surface of the titanium sheet (BMP2-CS-Ti). At the same time, we also release the BMP-2 (BMP2-Ti) or CS (CS-Ti) directly on the surface of the titanium sheet after the alkylation as half of the BMP-2 on the.BMP2-CS-Ti surface, proving that the CS coating is a good BMP-2 carrier. The bone induction ability of BMP2-CS-Ti, BMP2-Ti, CS-Ti and pristine-Ti was detected by culture of bone marrow mesenchymal stem cells (BMSCs) in vitro. The results showed that BMP2-CS-Ti was more capable of promoting cell differentiation than the other three groups. It indicated that the CS/BMP-2 Co coating was stable, maintained the activity of BMP-2, and had good bone induction ability. The antibacterial experiment proved CS/BMP-2 common. The coating has good antibacterial effect on Escherichia coli and Staphylococcus albus.
【學(xué)位授予單位】：西南交通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R318.08

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