本文選題：真絲縫合線 + 納米水凝膠��； 參考：《東華大學(xué)》2016年碩士論文

【摘要】：醫(yī)用真絲縫合線由于其穩(wěn)定、易操作的特性成為了目前社會(huì)上廣泛使用的縫合線之一。但是真絲縫合線作為一種不可吸收縫合線自身對(duì)細(xì)菌、病毒等沒(méi)有抑菌作用,編織結(jié)構(gòu)的縫隙以及絲線內(nèi)部單纖維間隙為細(xì)菌提供隱匿場(chǎng)所,這些都增加了手術(shù)部位感染(SSIs)的概率。為了賦予真絲縫合線良好的抗菌性能,在一定范圍內(nèi)阻隔細(xì)菌與手術(shù)切口的接觸,從而減少SSIs的發(fā)生概率。因此,研究具有抗菌性能的醫(yī)用真絲縫合線具有重要的臨床應(yīng)用價(jià)值。本文采用新型生物可降解賴氨酸聚酯氨納米水凝膠作為抗菌劑載體,基于常用醫(yī)用高�？咕鷦┑恼{(diào)查分析選擇了抗菌劑三氯生,通過(guò)化學(xué)接枝的方法將納米水凝膠接枝在真絲縫合線的表面。制備了能夠防止外科手術(shù)感染(SSIs)的長(zhǎng)效抗菌納米水凝膠接枝真絲縫合線。另外,通過(guò)抗菌性能試驗(yàn)評(píng)價(jià)了載三氯生的納米水凝膠接枝真絲縫合線(TLNGS)的抗菌性能�？咕阅茉囼�(yàn)的前四天觀察到TLNGS在瓊脂培養(yǎng)基上具有穩(wěn)定的抑菌圈�？咕鷾y(cè)試的結(jié)果表明接枝縫合線具有可以預(yù)防傷口早期感染的功能。與此同時(shí),化學(xué)接枝處理后縫合線的物理機(jī)械功能受到的影響極小可以忽略不計(jì)。具有生物相容性以及生物降解功能的賴氨酸基水凝膠作為藥物載體接枝在真絲縫合線表面并且提供了一種新的有效的通用抑菌性處理方式。制備載藥水凝膠,通過(guò)酶降解賴氨酸水凝膠,自組裝形成載藥納米水凝膠,通過(guò)對(duì)載藥納米水凝膠的抗菌功能評(píng)價(jià),證實(shí)了水凝膠無(wú)抗菌功能以及抗菌劑三氯生的抗菌有效性;進(jìn)一步研究了化學(xué)接枝后真絲縫合線的抗菌持久性,并與國(guó)外臨床抗菌縫合線產(chǎn)品進(jìn)行了抗菌持久性的對(duì)比研究;為了研究不同交聯(lián)密度水凝膠對(duì)縫合線抗菌功能的影響,選擇了兩種不同交聯(lián)密度水凝膠:水凝膠4-lys-4(1.5:1)以及4-lys-4(2:1),對(duì)縫合線進(jìn)行接枝處理,制成了2種抗菌縫合線;對(duì)兩種抗菌納米水凝膠進(jìn)行藥物緩釋,對(duì)接枝后的縫合線進(jìn)行sem分析以及抗菌緩釋性能測(cè)試,以及物理機(jī)械性能的相關(guān)評(píng)價(jià)。本課題得出的主要結(jié)論如下:水凝膠4-lys-4(1.5:1)、4-lys-4(2:1)采用胰蛋白酶生物降解7天后的納米尺度分別約為60nm和90nm。兩種水凝膠的粒徑分布窄,有利于抗菌劑穩(wěn)定的緩釋速率;交聯(lián)密度大的4-lys-4(2:1)載藥量高;載藥納米水凝膠對(duì)金黃色葡萄球菌和大腸桿菌均具有優(yōu)異的抗菌功能,未載藥水凝膠對(duì)金黃色葡萄球菌以及大腸桿菌無(wú)抑菌功能;化學(xué)接枝后的真絲縫合線力學(xué)性能、縫合線的直徑以及編織結(jié)構(gòu)基本沒(méi)有改變,符合標(biāo)準(zhǔn)。通過(guò)sem觀察,與接枝前的真絲縫合線相比,抗菌真絲縫合線表面被均勻接枝了具有長(zhǎng)效抑菌功能的抗菌納米水凝膠粒子;兩種接枝型抗菌縫合線具有優(yōu)異的抗菌持久性,相同測(cè)試條件下與國(guó)外的臨床用抗菌縫合線相比,具有更加持久的抗菌功能。且接枝交聯(lián)密度越大的抗菌納米水凝膠粒子的真絲縫合線的藥物緩釋抗菌功能越好。
[Abstract]:As a result of its stability and easy operation, medical silk suture has become one of the most widely used sutures in the society. However, the silk suture line, as an inabsorbable suture, has no bacteriostasis for bacteria, virus and so on. The probability of surgical site infection (SSIs) is increased. In order to give the good antibacterial properties of the silk suture line, the contact between the bacteria and the surgical incision is blocked in a certain range, thus reducing the probability of the occurrence of SSIs. Therefore, the study of the medical silk suture with antibacterial properties is of great importance in clinical application. The lysine polyester ammonia nano hydrogel is used as an antiseptic carrier. Based on the investigation and analysis of the antibacterial agents in medical colleges and universities, three chlorinated antibacterial agents are selected and the nano hydrogels are grafted onto the surface of the silk suture line by chemical grafting. The grafting of long effective antibacterial nanosels that can prevent the surgical infection (SSIs) is prepared. In addition, the antibacterial properties of three chlorinated nano hydrogels grafted with silk suture line (TLNGS) were evaluated through antibacterial properties test. The antibacterial properties of TLNGS were observed on the agar medium four days ago. The results of antimicrobial test showed that the branch suture could prevent early infection of the wound. At the same time, the physical and mechanical functions of the suture line after chemical grafting are negligible. The lysine based hydrogels with biocompatibility and biodegradability are grafted on the surface of the silk suture line as a drug carrier and provide a new and effective general antibacterial treatment method. Drug loading hydrogels were used to degrade lysine hydrogels by enzymes and self-assembled to form drug loaded nanosels. By evaluating the antibacterial function of the drug loaded nanosels, the antibacterial functions of the hydrogels and the antibacterial effectiveness of three chlorinated antimicrobial agents were confirmed, and the antibacterial durability of the silk suture line after chemical grafting was further studied, and it was associated with foreign presence. In order to study the antiseptic durability of the bed antibacterial suture products, two different crosslinked density hydrogels, hydrogels 4-lys-4 (1.5:1) and 4-lys-4 (2:1) were selected to study the effect of different crosslinking density hydrogels on the antibacterial function of the suture line, and 2 kinds of antibacterial suture lines were made and two kinds of antibacterial suture lines were made. The nano hydrogel carried out the drug release, the SEM analysis of the suture line after the grafting, the antibacterial release performance test, and the related evaluation of the physical and mechanical properties. The main conclusions are as follows: the hydrogel 4-lys-4 (1.5:1), 4-lys-4 (2:1) with trypsin degradation 7 days after the degradation of the nano scale of about 60NM and 90nm., respectively, two species The particle size distribution of the hydrogels is narrow, which is beneficial to the stable release rate of the antibacterial agent; the 4-lys-4 (2:1) with large crosslink density has high drug loading; the drug loaded nano hydrogel has excellent antibacterial function to Staphylococcus aureus and Escherichia coli, and the unloaded hydrogel has no bacteriostasis on Staphylococcus aureus and Escherichia coli. The mechanical properties of the silk suture, the diameter of the suture line and the knitting structure are basically unchanged, which conform to the standard. Compared with the silk suture before grafting, the surface of the antibacterial silk suture line is uniformly grafted with antibacterial nanoscale hydrogel particles with long effective bacteriostasis, and the two kinds of graft antibacterial suture lines have excellent antibacterial properties through SEM. Under the same test conditions, the antibacterial function of the antibacterial nano hydrogel particles with greater graft crosslinking density is better than that of the clinical antibacterial suture in foreign countries.
【學(xué)位授予單位】：東華大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：TQ427.26;R318.08

【共引文獻(xiàn)】

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本文編號(hào)：1948494