本文選題：缺鈣羥基磷灰石 + 骨修復(fù)材料�。� 參考：《華東理工大學(xué)》2012年碩士論文

【摘要】：本課題包括鎂替代磷酸鈣骨水泥(MCPC)和介孔硅酸鈣鎂(OMC)兩種鈣鎂基骨修復(fù)材料的研究。 第一部分將活性氧化鎂(MgO)與磷酸四鈣(Ca4(PO4)2O),磷酸氫鈣(CaHPO4)按照一定比例混合制備出新型的鎂替代磷酸鈣骨水泥。通過顆粒溶出的方法制備出MCPC多孔支架,支架材料在體外測試中表現(xiàn)出良好的生物降解性和細(xì)胞相容性。將MCPC多孔支架植入兔股骨缺損,支架表現(xiàn)出優(yōu)良的生物相容性,隨著植入時間的增加,材料逐步降解,12周時,材料完全降解,缺損區(qū)域被新骨所替代,材料顯示出了優(yōu)良的降解性和成骨能力。 第二部分利用溶膠-凝膠法制備出了介孔硅酸鈣鎂材料,該材料具有規(guī)整的7nm左右的介孔孔道,比表面積高達(dá)1017 m2/go OMC在Tris-HCl溶液中具有良好的溶解性,在模擬體液中浸泡7天后,表面有大量磷灰石生成,顯示出了優(yōu)良的體外生物活性。將材料植入兔股骨缺損,隨著植入時間的增長,新骨不斷生成,OMC潰散成小的碎片,逐步降解,12周后,骨缺損基本被修復(fù)。對OMC的吸附與釋放藥物及大分子蛋白的性能進(jìn)行了研究,結(jié)果表明,跟對照組的硅酸鈣鎂(CMS)相比,OMC材料能夠吸附大量的藥物以及一定量的大分子蛋白,且對吸附的藥物／蛋白有明顯的緩釋效果。 另外,對MCPC多孔支架和OMC的體內(nèi)動物實(shí)驗(yàn)所獲得的樣本利用上海同步輻射光源進(jìn)行生物醫(yī)學(xué)成像研究,結(jié)果清晰直觀的顯示了材料逐步降解,新骨逐漸生成的過程。實(shí)驗(yàn)表明MCPC和OMC都是很有潛力的骨修復(fù)生物材料。
[Abstract]:In this paper, two kinds of calcium and magnesium based bone repair materials, MCPC (calcium phosphate cement) and OMC (mesoporous calcium silicate), are studied. In the first part, a new type of calcium phosphate cement was prepared by mixing active magnesium oxide (MgO) with tetracalcium phosphate (Ca4CO4PO4), calcium phosphate (CaHPO4) and calcium phosphate (CaHPO4) according to a certain proportion. MCPC porous scaffolds were prepared by particle dissolution method. The scaffolds showed good biodegradability and cytocompatibility in vitro. The MCPC porous scaffold was implanted into rabbit femur defect. The scaffold showed excellent biocompatibility. With the increase of implantation time, the material degraded completely at 12 weeks, and the defect area was replaced by new bone. The material showed excellent biodegradability and osteogenic ability. In the second part, mesoporous calcium silicate magnesium was prepared by sol-gel method. The material has regular mesoporous channels about 7nm, and its specific surface area is up to 1017 m2/go OMC. It has good solubility in Tris-HCl solution, and is soaked in simulated body fluid for 7 days. A large amount of apatite was formed on the surface, which showed excellent bioactivity in vitro. The material was implanted into the rabbit femur defect. With the increase of the implantation time, the new bone burst into small fragments. After 12 weeks of degradation, the bone defect was basically repaired. The adsorption and release of drugs and the properties of macromolecular proteins were studied. The results showed that the OMC materials could adsorb a large amount of drugs and a certain amount of macromolecular proteins compared with the control group. And the drug / protein adsorbed has obvious slow release effect. In addition, the biomedical imaging of MCPC porous scaffold and OMC in vivo animal experiment was studied by using Shanghai Synchrotron radiation source. The results clearly and directly showed the process of material degradation and the gradual formation of new bone. Both MCPC and OMC are potential biomaterials for bone repair.
【學(xué)位授予單位】：華東理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08

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