本文選題：碳纖維復(fù)合材料 + 生物相容性��； 參考：《山西醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:驗(yàn)證骨誘導(dǎo)碳纖維復(fù)合材料急性期的生物相容性。方法:1.細(xì)胞毒性實(shí)驗(yàn):采用材料浸提液培養(yǎng)細(xì)胞,觀察其生長(zhǎng)情況,用CCK-8試劑盒測(cè)量細(xì)胞的吸光度值并計(jì)算細(xì)胞相對(duì)增殖率;2.急性毒性實(shí)驗(yàn):通過(guò)腹腔注射浸提液,觀察ICR小鼠活動(dòng)情況;3.微核實(shí)驗(yàn):通過(guò)腹腔注射浸提液,檢測(cè)ICR小鼠骨髓中的微核率。4.染色體畸變實(shí)驗(yàn):通過(guò)腹腔注射浸提液,并用秋水仙素處理后,檢測(cè)ICR小鼠染色體畸變情況。5.皮內(nèi)刺激實(shí)驗(yàn):將浸提液注入新西蘭大白兔背部皮下,觀察皮膚反應(yīng);6.熱原反應(yīng)實(shí)驗(yàn):將浸提液通過(guò)耳緣靜脈注入新西蘭大白兔體內(nèi),觀察體溫變化;7.溶血實(shí)驗(yàn):將浸提液加入新西蘭大白兔抗凝血中,觀察溶血情況并測(cè)量吸光度值;8.致敏實(shí)驗(yàn):將浸提液注入豚鼠背部皮下誘導(dǎo),在腹側(cè)激發(fā)致敏,觀察皮膚情況及病理變化。結(jié)果:1.細(xì)胞毒性實(shí)驗(yàn):細(xì)胞長(zhǎng)梭形,生長(zhǎng)狀況良好,A、B、C組在不同時(shí)間段的相對(duì)增殖率均75%;2.急性毒性實(shí)驗(yàn):A、B、C組的小鼠活動(dòng)良好,無(wú)不良反應(yīng);3.微核實(shí)驗(yàn):A、B、C組的微核率分別為1.40‰、1.80‰、1.20‰;4.染色體畸變實(shí)驗(yàn):A、B、C組無(wú)染色體發(fā)生畸變;5.皮內(nèi)刺激實(shí)驗(yàn):A、B、C組的平均原發(fā)刺激指數(shù)均小于0.4;6.熱原反應(yīng)實(shí)驗(yàn):A、B、C組的體溫升高值均0.6℃,并且每組3只兔子體溫升高值總數(shù)1.4℃;7.溶血實(shí)驗(yàn):A、B、C組溶血率均小于標(biāo)準(zhǔn)的5%;8.致敏實(shí)驗(yàn):A、B、C組的致敏率為0,并且病理切片未見(jiàn)異常。結(jié)論:骨誘導(dǎo)碳纖維復(fù)合材料人工顱骨板無(wú)細(xì)胞毒性,不引起急性毒性反應(yīng),無(wú)遺傳毒性,對(duì)皮膚無(wú)刺激,不會(huì)引起機(jī)體發(fā)熱,無(wú)致敏性,符合國(guó)家醫(yī)用材料臨床應(yīng)用的標(biāo)準(zhǔn)。
[Abstract]:Objective: to verify the biocompatibility of bone-induced carbon fiber composites in acute stage. Method 1: 1. Cytotoxicity test: the cells were cultured in the material extract, the growth of cells was observed, the absorbance value of cells was measured by CCK-8 kit and the relative proliferation rate of cells was calculated. Acute toxicity test: the activity of ICR mice was observed by intraperitoneal injection of extractant. Micronucleus test: the micronucleus rate in bone marrow of ICR mice was detected by intraperitoneal injection of extract. 4. 4. Chromosome aberration test: the chromosome aberration of ICR mice was detected by intraperitoneal injection of extract and colchicine treatment. Intradermal stimulation: the extract was injected subcutaneously into the back of New Zealand white rabbits and the skin reaction was observed. Pyrogen reaction experiment: the extract was injected into New Zealand white rabbits through auricular vein to observe the change of body temperature. Hemolysis experiment: the extract was added to the anticoagulant of New Zealand white rabbit, the hemolysis was observed and the absorbance value was measured. Sensitizing experiment: the extract was injected into the back of guinea pig to induce subcutaneously, and the skin condition and pathological changes were observed. The result is 1: 1. Cytotoxicity test: the cells were long fusiform and the growth condition was good. The relative proliferation rate of group C was 75 ~ 2 / 2 at different time points. In the acute toxicity test, the mice in the group B: AZB C had good activity and had no adverse reactions (P < 0. 05). The micronucleus rate of micronucleus test in group C was 1.40 鈥，

本文編號(hào)：1880264