本文選題：羥基磷灰石 + 納米銀�。� 參考：《新疆醫(yī)科大學》2015年碩士論文

【摘要】：目的：探討制備人工關節(jié)假體表面納米銀復合涂層材料的最佳參數(shù),觀測銀離子在模擬體液中的緩釋性能,評價復合材料組織相容性及動物體內(nèi)的抗菌性能。方法：應用脈沖電化學方法,在鈦金屬表面沉積羥基磷灰石/納米銀復合涂層,并對其形貌、空間構象、組成進行表征分析。將材料浸泡在模擬體液中,采用原子能吸收光譜法,檢測不同時間點溶液銀離子濃度。按照按國家和ISO的檢測標準,進行組織相容性實驗。成年新西蘭兔48只,隨機分為實驗A、B、C三組(n=16)。行兔全膝關節(jié)置換術,實驗A、B組植入不同銀離子濃度電解液沉積的人工關節(jié)假體復合材料,實驗C組植入普通人工關節(jié)假體。建立兔全膝關節(jié)置換術后金黃色葡萄球菌感染模型,評價復合材料在動物體內(nèi)抗菌性能。結果：0.5HA/Ag復合涂層銀含量為2.75wt%；第1d銀離子釋放量占30d總量的45.55%；溶血率為1.64%,體外對金黃色葡萄球菌的抗菌率為95.3%。1.0HA/Ag復合涂層銀含量為8.83wt%；第1d銀離子釋放量占30d總量的48.10%；溶血率為2.24%；體外對金黃色葡萄球菌的抗菌率為97.6%。動物感染模型中,實驗A、B組體溫、血C-反應蛋白、血沉、血漿降鈣素原均低于實驗C組,組間差異有統(tǒng)計學意義(P0.05)。感染率：實驗A組18.75%(3/16),實驗B組12.50%(2/16),實驗C組100%(16/16),組間差異有統(tǒng)計學意義(P0.05)；實驗A、B組之間感染率差異無統(tǒng)計學意義(P0.05),實驗組與對照組比較,感染率差異有統(tǒng)計學意義(P0.05)。結論：羥基磷灰石/納米銀復合涂層是由納米針狀羥基磷灰石和點狀的銀顆粒組成,銀顆粒分布均勻,無團聚現(xiàn)象。在觀察期間,復合材料的緩釋作用可維持有效的抗菌濃度,釋放總量低于細胞的毒性濃度。納米銀復合鈦涂層材料組織相容性良好。體外對金黃色葡萄球菌的抗菌率在95%以上。羥基磷灰石/納米銀復合涂層人工關節(jié)假體在動物體內(nèi)對金黃色葡萄球菌具有良好的抗菌活性。
[Abstract]:Aim: to investigate the optimal parameters for preparing nano-silver composite coating materials on artificial joint prosthesis, observe the slow release properties of silver ions in simulated body fluid, and evaluate the tissue compatibility and antibacterial properties of the composite materials in vivo. Methods: the hydroxyapatite / nano-silver composite coating was deposited on the surface of titanium by pulsed electrochemical method. The morphology, spatial conformation and composition of the coating were characterized. The material was immersed in simulated body fluid and the concentration of silver ions in solution at different time points was determined by atomic absorption spectrometry. According to the national and ISO standards, the histocompatibility test was carried out. A total of 48 adult New Zealand rabbits were randomly divided into three groups: group A (n = 16). Total knee arthroplasty was performed in rabbits. In group A, artificial joint composites deposited with different silver ion concentration electrolyte were implanted, and in group C, common artificial joint prostheses were implanted. A rabbit model of staphylococcus aureus infection after total knee arthroplasty was established to evaluate the antibacterial properties of composite materials in vivo. Results the silver content of 0.5 HA / Ag composite coating was 2.75 wtt.The silver ion release in the first day accounted for 45.55% of the total amount in 30 days, the hemolysis rate was 1.64 and the antibacterial rate against Staphylococcus aureus in vitro was 8.83 wt.The silver release amount in the first day accounted for 48.10% of the total amount of silver ion release in 30 days. The hemolysis rate was 2.24% and the antibacterial rate against Staphylococcus aureus in vitro was 97.6%. In the model of animal infection, the body temperature, serum C-reactive protein, erythrocyte sedimentation rate and plasma procalcitonin in group A were lower than those in group C, and the difference between the two groups was statistically significant (P 0.05). The infection rate of group A was 18.75 / 16, that of group B was 12.50 / 16, that of group C was 100 / 16 / 16, and that of group A was not significantly different from that of group A (P 0.05), and that of group A was significantly higher than that of group B (P 0.05). Conclusion: hydroxyapatite / nano-silver composite coating is composed of needle-like hydroxyapatite and dot silver particles with uniform distribution and no agglomeration. During the observation period, the sustained release of the composite could maintain the effective antibacterial concentration and the total release amount was lower than the cytotoxic concentration. The nano-silver composite titanium coating material has good histocompatibility. The antibacterial rate against Staphylococcus aureus in vitro was over 95%. Hydroxyapatite / nano-silver composite coating artificial joint prosthesis has good antibacterial activity against Staphylococcus aureus in vivo.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R318.17

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本文編號：1854848