本文選題：CdTe/CdS核/殼量子點(diǎn) + 熒光探針��； 參考：《東北師范大學(xué)》2012年碩士論文

【摘要】：將水溶性熒光量子點(diǎn)應(yīng)用于腫瘤細(xì)胞成像和熒光共振能量轉(zhuǎn)移這兩個領(lǐng)域，是近年來國內(nèi)外研究的熱點(diǎn)。在本課題中嘗試分別利用不同種類，不同熒光發(fā)射波長的量子點(diǎn)應(yīng)用在這兩個領(lǐng)域中，主要研究內(nèi)容和結(jié)果如下： 1.在以單層CdTe量子點(diǎn)為核的基礎(chǔ)上，通過水相合成的方法制備了最大發(fā)射波長分別為545nm和600nm的CdTe/CdS核/殼量子點(diǎn)。通過紫外-可見吸收光譜，熒光光譜，XRD，透射電鏡等表征手段，證實(shí)了CdTe/CdS量子點(diǎn)的核/殼結(jié)構(gòu)并且與單層CdTe量子點(diǎn)相比具有更強(qiáng)的光穩(wěn)定性及熒光量子產(chǎn)率。通過與抗腫瘤藥物5-氟尿嘧啶（5-FU），P-gp抑制劑他莫西芬(TAM)以及生物素偶聯(lián)，制備了CdTe/CdS(545nm)-5-FU及bio-CdTe/CdS(600nm)-TAM兩種新型熒光探針，并進(jìn)一步探討了其相互作用的機(jī)理。通過將兩種熒光探針對腫瘤細(xì)胞進(jìn)行標(biāo)記，我們發(fā)現(xiàn)bio-CdTe/CdS-TAM能夠靶向追蹤細(xì)胞膜表面上的P-gp，并且TAM成功抑制了P-gp對抗腫瘤藥物的外排作用，使抗腫瘤藥物5-FU隨著CdTe/CdS（545nm）進(jìn)入到了細(xì)胞核內(nèi)而未被排出。這使得P-gp的抑制作用能夠可視化，擴(kuò)大了量子點(diǎn)在腫瘤細(xì)胞成像和治療領(lǐng)域的應(yīng)用。 2.將最大發(fā)射波長分別為530nm和610nm的CdTe量子點(diǎn)，分別與兩種生物分子——親合素（Avi）和生物素（Bio）進(jìn)行共價偶聯(lián)，并分別考察了pH值，反應(yīng)溫度及反應(yīng)物配比對偶合物的影響。借助Avi與Bio之間的強(qiáng)親和力及反應(yīng)的高度專一性，，成功的構(gòu)建了熒光共振能量轉(zhuǎn)移（FRET）體系，并考察了不同供體和受體摩爾比對FRET效率的影響，這為FRET技術(shù)在藥物定量分析領(lǐng)域的應(yīng)用奠定了基礎(chǔ)。同時，利用高效液相色譜（HPLC）這一快速、靈敏、可靠的檢測方法分析偶合物的生成及FRET的構(gòu)建，大大提高了分析的準(zhǔn)確性和靈敏性。
[Abstract]:The application of water-soluble fluorescent quantum dots in tumor cell imaging and fluorescence resonance energy transfer is a hot topic in recent years. In this paper, we try to use different kinds of quantum dots with different emission wavelengths in these two fields. The main research contents and results are as follows: 1. Based on monolayer CdTe quantum dots, CdTe/CdS core / shell quantum dots with maximum emission wavelengths of 545nm and 600nm were prepared by water phase synthesis. The core / shell structure of CdTe/CdS quantum dots was confirmed by UV-Vis absorption spectra, fluorescence spectra and transmission electron microscopy, and the photoluminescence stability and fluorescence quantum yield of CdTe/CdS quantum dots were better than those of monolayer CdTe QDs. Two novel fluorescent probes, CdTe/CdS(545nm)-5-FU and bio-CdTe/CdS(600nm)-TAM, were prepared by coupling with the anti-tumor drug 5-fluorouracil (5-FU) P-gp inhibitor tamoxifen (TAM) and biotin, and the mechanism of their interaction was further discussed. By labeling tumor cells with two fluorescent probes, we found that bio-CdTe/CdS-TAM was able to target P-gp on the surface of cell membrane, and TAM successfully inhibited the efflux of P-gp against tumor drugs. The antitumor drug 5-FU enters the nucleus with CdTe / CdSU 545 nm and is not excreted. This makes the inhibition of P-gp visible and expands the application of quantum dots in tumor cell imaging and treatment. 2. The CdTe quantum dots with the maximum emission wavelengths of 530nm and 610nm were covalently coupled with two biomolecules, avion and biotin, respectively. The effects of pH value, reaction temperature and reactant ratio on the conjugate were investigated. Based on the strong affinity between Avi and Bio and the high specificity of the reaction, the fluorescence resonance energy transfer (FRET) system was successfully constructed, and the effects of different donor and receptor molar ratios on the FRET efficiency were investigated. This laid a foundation for the application of FRET technology in the field of drug quantitative analysis. At the same time, the rapid, sensitive and reliable detection method of HPLC was used to analyze the formation of coupling compounds and the construction of FRET, which greatly improved the accuracy and sensitivity of the analysis.
【學(xué)位授予單位】：東北師范大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.51;O657.3

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 劉念;耿小平;熊茂明;;P-糖蛋白抑制劑的研究進(jìn)展[J];國外醫(yī)學(xué).藥學(xué)分冊;2006年02期

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