本文選題：院內(nèi)感染 + 絲素蛋白　； 參考：《重慶理工大學(xué)》2012年碩士論文

【摘要】：院內(nèi)感染是各醫(yī)院內(nèi)普遍存在的問(wèn)題，主要是各種細(xì)菌感染，易造成患者的進(jìn)一步健康損害。為此，開(kāi)發(fā)具有抗菌性能的醫(yī)療器械表面涂裝材料、設(shè)備具有重要意義。 從絲素蛋白作為保護(hù)蠶順利度過(guò)蛹期而羽化成蛾的生理功能來(lái)看，絲素蛋白具有微調(diào)節(jié)周圍環(huán)境的功用，，可抑制細(xì)菌繁殖，具備抑菌性。但是純?cè)偕Q絲素蛋白材料的成型性及柔韌性能較差，且價(jià)格昂貴，其應(yīng)用受到限制。本課題擬將生物相容性良好的蠶絲素蛋白與成型性好的聚乙烯醇結(jié)合起來(lái)，開(kāi)發(fā)一種具抗菌性的新型材料。研究采用高濃度的鹽溶液溶解絲素纖維，并用中性蛋白酶酶解制備納米級(jí)絲素蛋白(SFP)，再采用水相共混，將所制得的絲素與聚乙烯醇（PVA）按照不同配比共混并成型，制備出了納米絲素蛋白和聚乙烯醇的共混膜（SFP/PVA）。對(duì)SFP/PVA共混膜進(jìn)行了力學(xué)性能的表征，證明了SFP/PVA共混膜的拉伸強(qiáng)度、能承受的最大力與斷裂強(qiáng)度均較好。其中SFP/PVA配比為30/70的膜的力學(xué)性能最好。采用平板菌落計(jì)數(shù)的方法對(duì)其進(jìn)行了抗菌性能測(cè)試，結(jié)果顯示部分比例的SFP/PVA共混膜對(duì)大腸桿菌、金黃色葡萄球菌的抗菌率可達(dá)到60%以上，而對(duì)銅綠色假單胞菌的抗菌效果也可達(dá)到30%以上。血液相容性試驗(yàn)結(jié)果表明SFP/PVA共混膜的溶血率低于5%，符合生物材料溶血試驗(yàn)要求；并且SFP/PVA共混膜具備一定的抗凝血效果；血小板消耗率低于20%，符合與血液接觸的材料的使用要求。SFP/PVA配比為30/70的膜的血液相容性比其它幾組配比好。細(xì)胞毒性試驗(yàn)結(jié)果表明SFP/PVA共混膜均無(wú)細(xì)胞毒性，且SFP/PVA配比為30/70的膜具有促細(xì)胞生長(zhǎng)的作用。初步證明了SFP/PVA共混膜作為醫(yī)療器械表面涂裝材料或醫(yī)用導(dǎo)管材料的應(yīng)用前景。
[Abstract]:Nosocomial infection is a common problem in hospitals. Therefore, it is of great significance to develop surface coating materials for medical devices with antibacterial properties. As a physiological function to protect silkworm from pupal stage, silk fibroin has the function of microregulating the surrounding environment, inhibiting the propagation of bacteria and having bacteriostasis. However, the pure regenerated silk protein material has poor formability and flexibility, and its application is limited because of its high price. In this paper, a new antibacterial material was developed by combining silk fibroin with polyvinyl alcohol with good biocompatibility. In this paper, silk fibroin fibers were dissolved in high concentration salt solution and prepared by enzymatic hydrolysis of neutral protease. The prepared silk fibroin and polyvinyl alcohol (PVA) were blended and formed according to the different ratio of silk fibroin and polyvinyl alcohol (PVA), and then water phase was used to blend the silk fibroin and polyvinyl alcohol (PVA). A blend of nano-silk fibroin and polyvinyl alcohol was prepared. The mechanical properties of the SFP/PVA blend film were characterized. It was proved that the tensile strength of the SFP/PVA blend film was the most powerful and the breaking strength of the SFP/PVA blend film was better. The film with SFP/PVA ratio of 30 / 70 has the best mechanical properties. The antibacterial properties of SFP/PVA blend membrane were tested by the method of plate colony count. The results showed that the antibacterial rate of Staphylococcus aureus could reach more than 60% against Escherichia coli. The antimicrobial effect against Pseudomonas copper-green was more than 30%. The results of blood compatibility test showed that the hemolysis rate of SFP/PVA blend membrane was lower than 5, which met the requirements of biomaterial hemolysis test, and the SFP/PVA blend membrane had a certain anticoagulant effect. The platelet consumption rate was lower than 20%, which met the requirements of the use of materials in contact with blood. The membrane with a ratio of 30 / 70 to SFP / PVA had better blood compatibility than the other groups. The results of cytotoxicity test showed that the SFP/PVA blend membrane had no cytotoxicity, and the membrane with SFP/PVA ratio of 30 / 70 had the effect of promoting cell growth. The application prospect of SFP/PVA blend film as medical instrument surface coating material or medical conduit material was preliminarily proved.
【學(xué)位授予單位】：重慶理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08

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