本文選題：脂肪移植 + 藻酸鈣�。� 參考：《浙江大學(xué)》2012年碩士論文

【摘要】：背景： 自體顆粒脂肪是一種良好的組織充填材料,取材方便,無(wú)組織排異性,采用注射方式進(jìn)行游離移植,因而具有操作簡(jiǎn)易,創(chuàng)傷較小的優(yōu)點(diǎn)。臨床上,技術(shù)被廣泛應(yīng)用于面部及體表凹陷的充填,乳房形態(tài)的增大等整形美容領(lǐng)域。我們的前期研究證實(shí)：加速移植體的血管重建是提高顆粒脂肪移植存活率的關(guān)鍵,從而促血管生長(zhǎng)因子成為了研究的重點(diǎn)。而如何選擇組織相容性良好,適時(shí)降解,可緩釋,無(wú)毒性的生物材料,構(gòu)建生長(zhǎng)因子復(fù)合載體,使生長(zhǎng)因子可穩(wěn)定釋放,持續(xù)作用于靶組織和細(xì)胞是研究的關(guān)鍵和該領(lǐng)域的發(fā)展方向。 目的： 觀察血管內(nèi)皮生長(zhǎng)因子(vascular endothelial growth factor, VEGF)與藻酸鈣復(fù)合的緩釋系統(tǒng)對(duì)自體脂肪移植成活率的影響。 方法： 從人體獲取脂肪,經(jīng)純化,消化后按加入物不同分成4組,即實(shí)驗(yàn)組(脂肪+藻酸鈣+20ng/ml VEGF)、藻酸鈣對(duì)照組(脂肪+藻酸鈣)、VEGF對(duì)照組(脂肪+20ng/ml VEGF)、空白對(duì)照組(單純脂肪)。各組混合物分別注射于裸鼠背部皮下。在3、6和12周分別取材.行移植物質(zhì)量、電鏡超微結(jié)構(gòu)、組織學(xué)染色和微血管CD34染色檢測(cè)。 結(jié)果： 實(shí)驗(yàn)組(藻酸鈣復(fù)合VEGF)的移植物重量和血管密度均高于對(duì)照組,具有統(tǒng)計(jì)學(xué)意義,其余各對(duì)照組間差別無(wú)統(tǒng)計(jì)學(xué)意義。 結(jié)論： 藻酸鈣為載體構(gòu)建的VEGF復(fù)合物能促進(jìn)移植后脂肪組織血管的生成從而提高脂肪游離移植的成活率。
[Abstract]:Background: Autologous granular fat is a kind of good tissue filling material, which is easy to take, and can be used for free transplantation by injection, so it has the advantages of simple operation and less trauma. Clinically, the technique has been widely used in the plastic and cosmetic fields such as facial and body surface concave filling, enlargement of breast shape and so on. Our previous studies have confirmed that accelerated vascular remodeling is the key to improve the survival rate of granular fat transplantation, thus promoting vascular growth factor has become the focus of research. However, how to select biomaterials with good histocompatibility, timely degradation, slow-release and non-toxicity, to construct the compound carrier of growth factor, so that the growth factor can be released stably. Continuous action on target tissues and cells is the key of research and the development direction of this field. Objective: To observe the effect of vascular endothelial growth factor (VEGF) combined with calcium alginate on the survival rate of autogenous fat transplantation. Methods: Fat was obtained from human body, purified, digested and divided into 4 groups: experimental group (calcium alginate 20ng/ml VEGF), calcium alginate control group (adipose 20ng/ml VEGF), blank control group (simple adipose acid). The mixture was injected subcutaneously into the back of nude mice. The materials were collected at 3 ~ 6 and 12 weeks, respectively. The grafts mass, ultrastructure of electron microscope, histological staining and microvascular CD34 staining were detected. Results: The graft weight and vascular density of the experimental group (calcium alginate combined with VEGF) were significantly higher than that of the control group, but there was no significant difference between the other control groups. Conclusion: The VEGF complex constructed with calcium alginate as carrier can promote the angiogenesis of adipose tissue and improve the survival rate of free fat transplantation.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類(lèi)號(hào)】：R318.08

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