本文選題：摻鍶羥基磷灰石 + 放電等離子燒結(jié)�。� 參考：《昆明理工大學(xué)》2017年碩士論文

【摘要】：本實(shí)驗(yàn)以碳酸氫銨作為造孔劑,在不使用粘結(jié)劑的前提下通過放電等離子燒結(jié)技術(shù)快速制備多孔純羥基磷灰石和摻鍶羥基磷灰石材料(摻鍶量為5wt%)。XRD圖譜表明燒結(jié)后摻鍶羥基磷灰石是具有與羥基磷灰石相同結(jié)構(gòu)的晶體,鍶的摻入未生成其它雜相。掃描電鏡顯示各材料在燒結(jié)過程中均形成了 200μm左右的孔隙,孔隙率約為40%,孔與孔之間相互貫通,能夠提供骨組織和血管等充足的長入空間。力學(xué)性能測試表明兩種材料的壓縮強(qiáng)度均在3MPa以上,接近于人松質(zhì)骨的壓縮強(qiáng)度,另外,鍶的摻入提高了羥基磷灰石的力學(xué)性能。LDH活性檢測表明兩種材料均無細(xì)胞毒性。兔P3代骨髓間充質(zhì)干細(xì)胞共培養(yǎng)實(shí)驗(yàn)表明羥基磷灰石摻入鍶有助于促進(jìn)骨髓間充質(zhì)干細(xì)胞的增殖、成骨分化和黏附。成骨細(xì)胞MG-63共培養(yǎng)實(shí)驗(yàn)表明羥基磷灰石摻入鍶有助于促進(jìn)成骨細(xì)胞的增殖、分化、黏附和遷移。兔橈骨骨缺損修復(fù)實(shí)驗(yàn)表明摻鍶羥基磷灰石具有良好的骨修復(fù)能力,植入8周后材料與宿主骨發(fā)生骨整合,材料表面大量編織骨生成,16周后材料基本降解,板狀骨塑形,骨重塑基本完成。體內(nèi)外實(shí)驗(yàn)表明多孔摻鍶羥基磷灰石材料具有無細(xì)胞毒性、良好的骨傳導(dǎo)性和生物降解性,是一種理想的骨缺損修復(fù)材料。
[Abstract]:In this experiment, ammonium bicarbonate was used as pore-forming agent.Rapid preparation of porous pure hydroxyapatite and strontium doped hydroxyapatite by spark plasma sintering without binder (strontium content is 5wt%).XRD diagram shows that strontium doped hydroxyapatite is associated with hydroxyl group).Apatite crystals of the same structure,The incorporation of strontium did not produce other heterogenous phases.Scanning electron microscopy (SEM) showed that about 200 渭 m pores were formed in the sintering process, and the porosity was about 40 渭 m. The porosity was about 40 渭 m, and the holes were interpenetrating with each other, which could provide sufficient long space for bone tissue and blood vessels.Mechanical properties test showed that the compressive strength of the two materials was above 3MPa, close to the compressive strength of human cancellous bone. In addition, the incorporation of strontium enhanced the mechanical properties of hydroxyapatite. The results showed that the two materials had no cytotoxicity.The co-culture of bone marrow mesenchymal stem cells of rabbit P3 generation showed that the incorporation of hydroxyapatite with strontium could promote the proliferation, osteogenic differentiation and adhesion of bone marrow mesenchymal stem cells.The co-culture of osteoblasts by MG-63 showed that the incorporation of strontium into hydroxyapatite could promote the proliferation, differentiation, adhesion and migration of osteoblasts.The repair experiment of rabbit radius bone defect showed that strontium doped hydroxyapatite had good bone repair ability. After 8 weeks of implantation, the material was integrated with the host bone, the material was basically degraded after 16 weeks of formation of a large number of braided bone on the surface of the material, and the plate bone was shaped.Bone remodeling was basically completed.In vitro and in vivo experiments showed that the porous strontium doped hydroxyapatite material had no cytotoxicity, good bone conduction and biodegradability, and was an ideal bone defect repair material.
【學(xué)位授予單位】：昆明理工大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R318.08

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 高建勇;田剛;朱強(qiáng);王少海;汪大林;;鍶摻雜改性羥基磷灰石的生物學(xué)性能研究[J];第二軍醫(yī)大學(xué)學(xué)報(bào);2016年02期

2 劉彪;李運(yùn)峰;胡靜;;含鍶羥基磷灰石對骨質(zhì)疏松癥大鼠的影響[J];中國骨質(zhì)疏松雜志;2015年05期

3 王輝;張建軍;;兔骨髓間充質(zhì)干細(xì)胞向成骨細(xì)胞誘導(dǎo)分化的不同培養(yǎng)方法[J];中國組織工程研究;2014年41期

4 張志升;徐勇;蘇劍生;劉偉才;肖云;;牙科陶瓷材料彎曲強(qiáng)度可靠性和亞臨界裂紋擴(kuò)展研究[J];實(shí)用口腔醫(yī)學(xué)雜志;2011年03期

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