本文選題：聚乳酸乙醇酸(PLGA)　切入點：云南白藥　出處：《南華大學(xué)》2013年碩士論文

【摘要】：目的1.通過實驗探索合適的云南白藥及PLGA材料配比、選擇簡單易行膜片制作方法。2.通過實驗研究云南白藥PLGA混合材料的生物相容性，及其對傷口愈合的影響。 方法第一部分：1.復(fù)合材料制備：將云南白藥與PLGA粉末材料按不同質(zhì)量配比（1:5,1:10,1:20）溶解于氯仿中攪拌成糊狀，再將其自然平鋪于玻璃板上。干燥后裁剪成1.5cm×1.5cm大小復(fù)合材料片，干燥冷藏備用。 2.體外降解：將不同配比的混合材料膜片放入模擬腸液內(nèi)，模擬腸液每星期更換，，并將降解裝置放置于37度恒溫箱中。于1、2、3、4、6、8周，6個時間點分別取出相應(yīng)膜片，干燥后計算膜片的失重率，并評價其降解情況。 第二部分：混合材料的生物相容性實驗：制備云南白藥PLGA混合材料浸提液后進(jìn)行急性毒性實驗、熱源實驗、溶血實驗、微核實驗，并通過肌肉內(nèi)埋置混合材料膜片，行手術(shù)切口周圍組織HE染色、Masson染色、羥脯氨酸測定等來評價云南白藥PLGA混合材料生物相容性，并與橡膠進(jìn)行對比、討論其對傷口愈合的影響。 結(jié)果1、各質(zhì)量配比的混合材料均在2-4周降解速率最快，降解約74-78%，6-8周降解放緩。 2、混合材料浸提液急性毒性實驗陰性、熱源實驗體溫上升均＜0.6℃、溶血率≤5%、微核率≤5‰。 3、動物實驗初步表明： ①HE染色中切口周圍可見多量炎性細(xì)胞浸潤，各配比的云南白藥與PLGA混合材料組較陽性橡膠對照組均較少； ②炎性反應(yīng)顯示：各配比混合材料組的在同一時間段與橡膠組對比具有顯著差異（p＜0.05），各配比材料組之間在同一時間段對比有顯著差異（p＜0.05），各配比材料組在不同時間對比有顯著差異（p＜0.05）； ③Masson染色中切口處膠原纖維為藍(lán)綠色，肌纖維及纖維素為紅色。7天時我們可見到炎癥細(xì)胞堆積，纖維細(xì)胞增生，少量膠原沉積，膠原纖細(xì)、稀疏。14天時切口處見大量膠原沉積，膠原粗大、密集。 結(jié)論1.我們可以通過調(diào)節(jié)云南白藥及PLGA材料之間的配比，使之具有不同的降解速度，挑選適合吻合器的要求的比例。 2.云南白藥PLGA混合材料是一種生物相容性良好，且可減少傷口炎性反應(yīng)、促進(jìn)傷口愈合的可降解材料。配比為1:5的混合材料組降解率稍快，且溶血率及微核率最低，此配比可能更適用于吻合器的制作。
[Abstract]:Objective 1.The suitable ratio of Yunnan Baiyao and PLGA materials was explored through experiments, and the simple and easy method of making diaphragm was chosen. 2.The biocompatibility of Yunnan Baiyao PLGA mixture and its effect on wound healing were studied experimentally.Method part one: one.Preparation of composite material: Yunnan white medicine and PLGA powder materials were dissolved in chloroform and mixed into chloroform at different mass ratio of 1: 5% 1: 1 1: 20) to form paste, and then they were naturally laid on glass plate.After drying, cut into 1.5cm 脳 1.5cm composite sheet, dry and refrigerate.2.In vitro degradation: the film of mixed material with different ratio is put into the simulated intestinal fluid, which is replaced weekly, and the degradation device is placed in the 37 degree thermostat.The corresponding diaphragm was taken out at 6 times after drying, and the weight loss rate of the diaphragm was calculated after drying and the degradation of the diaphragm was evaluated.The second part: the biocompatibility experiment of the mixture: the acute toxicity test, heat source test, hemolysis test, micronucleus test were carried out after the preparation of the extract of Yunnan Baiyao PLGA mixed material.The biocompatibility of Yunnan Baiyao PLGA mixture was evaluated by HE staining and hydroxyproline determination in the tissues around the incision and compared with rubber to discuss its effect on wound healing.Results 1. The degradation rate of the mixed materials with different mass ratios was the fastest in 2-4 weeks, and the degradation rate slowed down from 6-8 weeks to about 74-78 weeks.2, the acute toxicity test of the extraction solution of the mixed material was negative, the body temperature of the heat source test was less than 0.6 鈩

本文編號：1702506