本文選題：深低溫保存　切入點(diǎn)：復(fù)合組織　出處：《中國(guó)醫(yī)學(xué)工程》2012年05期

【摘要】：目的比較3種不同復(fù)溫方法對(duì)深低溫冷凍的復(fù)合組織中血管活性的影響,找出一種對(duì)復(fù)合組織中血管損傷最小的復(fù)溫方法。方法選用20只新西蘭大白兔隨機(jī)分成四組,切取雙側(cè)后肢(對(duì)照組為新鮮斷肢取血管),三個(gè)實(shí)驗(yàn)組標(biāo)本經(jīng)梯度降溫后放入液氮中保存7天。分別采用37℃水浴復(fù)溫組(A組)、空氣復(fù)溫組(B組)、-20℃冰復(fù)溫組(C組)方法進(jìn)行復(fù)溫。采用光鏡、免疫組化方法觀察血管內(nèi)皮細(xì)胞活性,結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果光鏡觀察,A組、B組內(nèi)皮細(xì)胞大片脫落,平滑肌細(xì)胞變形;而C組內(nèi)皮細(xì)胞和平滑肌細(xì)胞保存較好,更接近對(duì)照組;免疫組化檢測(cè)中,3種復(fù)溫方法血管內(nèi)皮中均可見VEGF及CD34呈斑片狀的弱表達(dá),-20℃冰復(fù)溫組(C組)VEGF及CD34的表達(dá)更接近對(duì)照組,高于37℃水浴復(fù)溫組(A組)及空氣復(fù)溫組(B組)(P0.05)。結(jié)論 3種不同復(fù)溫方法對(duì)深低溫冷凍的復(fù)合組織中血管活性影響不同,-20℃冰復(fù)溫法影響最小。
[Abstract]:Objective to compare the effects of three different rewarming methods on vascular activity in deep hypothermia and cryopreservation composite tissues, and to find out a rewarming method with minimal vascular damage in the composite tissues. Methods Twenty New Zealand white rabbits were randomly divided into four groups. Bilateral hindlimbs were removed (the control group was fresh amputated limb and blood vessel was removed. The specimens of three experimental groups were kept in liquid nitrogen for 7 days after gradient cooling. They were treated with 37 鈩，

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