本文選題：聚乳酸　切入點(diǎn)：膠原蛋白　出處：《山東輕工業(yè)學(xué)院》2012年碩士論文

【摘要】：聚乳酸（Poly(lactic acid)，PLA）由于具有良好的生物可降解性、生物相容性和力學(xué)性能，在醫(yī)用手術(shù)縫合線、注射用膠囊、微球等醫(yī)學(xué)領(lǐng)域得到廣泛應(yīng)用和認(rèn)可。但聚乳酸親水性較差，降低了其細(xì)胞相容性。另外聚乳酸相對(duì)分子量分布較寬，降解周期難以控制，降解中會(huì)導(dǎo)致局部酸性積累。因此，克服聚乳酸單一材料的性能缺陷，使其能更好的滿足某些醫(yī)學(xué)領(lǐng)域的應(yīng)用需求就顯得尤為重要。 本文以自制PLA為原料，以酰氯化為主，使用PCl_5、PCl_3作為酰氯化試劑，將PLA末端的羧酸基-COOH酰氯化成-COCl，再將膠原蛋白引入到PLA整體中，制備出膠原蛋白改性聚乳酸材料CPLA。 本文采用紅外光譜（FTIR）、熒光光譜、光電子能譜（XPS）、差示掃描量熱儀（DSC）以及茚三酮顯色分析等方法對(duì)改性聚乳酸進(jìn)行了表征，紅外譜圖分析顯示CPLA中出現(xiàn)了仲酰胺以及伯胺的N-H特征吸收峰，以PCl_5法制備的CPLA的特征峰最明顯；熒光光譜顯示采用異硫氰酸熒光素（FITC）標(biāo)記的CPLA在在510～550nm之間有較強(qiáng)的發(fā)射光譜，在450～500nm之間也有激發(fā)光譜，而FITC標(biāo)記的PLA則沒有吸收；XPS顯示，在CPLA中檢測(cè)到了N元素，而PLA中沒有；DSC分析表明，CPLA只有一個(gè)玻璃化轉(zhuǎn)變溫度；這些結(jié)論都表明了膠原蛋白已經(jīng)成功接枝到PLA中。 親水性測(cè)試結(jié)果顯示，相對(duì)于純PLA，CPLA的接觸角（61°）有所減小，而吸水率（3.49%）要明顯高于純PLA。通過降解過程特性粘度變化測(cè)試，發(fā)現(xiàn)，前三周CPLA和PLA的降解趨勢(shì)基本相同，但是從第四周開始CPLA的降解趨勢(shì)明顯要比PLA的穩(wěn)定、平緩。同時(shí)，降解過程失重率測(cè)試也說明，CPLA降解趨勢(shì)平穩(wěn)。而CPLA在降解過程中并未出現(xiàn)酸催化自加速降解行為。3T3細(xì)胞相容性測(cè)試顯示，通過顯微鏡形態(tài)觀察，發(fā)現(xiàn)CPLA基底上細(xì)胞多呈短梭形、三角形，，細(xì)胞密度高，并且大部分連接成片，細(xì)胞的生長(zhǎng)狀況要明顯好于PLA組和玻璃組。并且細(xì)胞增殖曲線也顯示，在CPLA上細(xì)胞增殖速率始終明顯高于PLA組。 本文研究結(jié)果說明：經(jīng)PCl_5法制備出的CPLA的親水性要明顯好于PLA，降解過程平穩(wěn)，沒有出現(xiàn)酸致自催化加速降解的現(xiàn)象。并且CPLA表現(xiàn)出較為出色細(xì)胞相容性。因此，本文通過酰氯化制備出的CPLA有望成為一種新型醫(yī)用高分子材料。
[Abstract]:Because of its good biodegradability, biocompatibility and mechanical properties, PLA has been widely used and recognized in medical fields such as surgical suture, injection capsules, microspheres and so on, but the hydrophilicity of polylactic acid is poor. In addition, the relative molecular weight distribution of polylactic acid is relatively wide, the degradation cycle is difficult to control, and the degradation will lead to local acid accumulation. So that it can better meet the needs of some medical applications is particularly important. In this paper, PLA was used as raw material, acyl chlorination was used as the main material, PCL _ (5) / PCL _ (3) was used as acyl chlorination reagent, carboxylic acid -COOH acyl chloride at the end of PLA was formed into -COCl, and collagen was introduced into PLA to prepare collagen modified poly (lactic acid) material. In this paper, the modified polylactic acid was characterized by FTIR, fluorescence, photoelectron spectroscopy (XPS), differential scanning calorimetry (DSC) and color analysis of ninhydrin. Infrared spectrum analysis showed that the N-H characteristic absorption peaks of paramide and primary amine appeared in CPLA, the characteristic peaks of CPLA prepared by PCl_5 method were the most obvious, and the fluorescence spectra showed that CPLA labeled with fluorescein isothiocyanate had a strong emission spectrum between 510~550nm. There were also excitation spectra between 450~500nm and PLA labeled with FITC. The N element was detected in CPLA, but there was only one glass transition temperature in PLA. These results indicate that collagen has been successfully grafted into PLA. The results of hydrophilic test showed that the contact angle (61 擄) of CPLA was decreased, but the water absorption was 3.49), which was obviously higher than that of pure PLA.The degradation trend of CPLA and PLA in the first three weeks was basically the same, by testing the change of intrinsic viscosity during the degradation process. But from the fourth week on, the degradation trend of CPLA was obviously more stable and gentle than that of PLA. At the same time, The weight loss rate test also showed that the degradation trend of CPLA was stable, but the acid-catalyzed autoaccelerated degradation behavior. 3T3 cell compatibility test showed that the cells on CPLA substrate were mostly short spindle shape. The cell density was high and most of the cells were connected into pieces. The growth status of the cells was obviously better than that of PLA and glass groups. The cell proliferation curve also showed that the cell proliferation rate in CPLA was always higher than that in PLA group. The results show that the hydrophilicity of CPLA prepared by PCl_5 method is obviously better than that of CPLA, the degradation process is stable, and there is no acid-induced autocatalytic accelerated degradation phenomenon, and CPLA has excellent cytocompatibility. In this paper, CPLA prepared by acyl chlorination is expected to be a new kind of medical polymer material.
【學(xué)位授予單位】：山東輕工業(yè)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08

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