本文選題：腎透析　切入點：溶血　出處：《中國組織工程研究》2017年26期

【摘要】：背景:傳統(tǒng)的透析膜多由有機聚合物材料合成,這些材料雖然能滿足治療需要,但材料孔徑尺寸范圍較大,微管不均勻,容易造成蛋白質在透析膜上淤塞,難以達到預期的治療效果。目的:分析二氧化鈦納米管生物透析膜的血液相容性。方法:根據(jù)陽極氧化法制備高強度的二氧化鈦納米管薄膜,利用HF氣體對納米管底部進行腐蝕,獲得兩端通透的二氧化鈦納米管陣列薄膜,將LLC-PK1細胞和ECV304細胞分別接種于二氧化鈦納米管陣列薄膜上,獲得兩種二氧化鈦納米管生物透析膜。(1)溶血實驗:將兩種二氧化鈦納米管生物透析膜及聚氨酯材料分別浸泡于生理鹽水與蒸餾水中,檢測溶血率;(2)動態(tài)凝血時間實驗:將涂過兔血的兩種二氧化鈦納米管生物透析膜及聚氨酯材料分別與氯化鈣混合,計算動態(tài)凝血時間;(3)血小板黏附實驗:將富血小板血漿分別滴于兩種二氧化鈦納米管生物透析膜及聚氨酯材料上,計算血小板黏附率。結果與結論:(1)溶血實驗:接種LLC-PK1細胞、ECV304細胞通透二氧化鈦納米管薄膜的溶血率均低于聚氨酯材料(0.30%,0.34%,0.56%,P0.05);(2)動態(tài)凝血時間實驗:接種LLC-PK1細胞、ECV304細胞通透二氧化鈦納米管薄膜20,40,70 min的動態(tài)凝血時間顯著長于聚氨酯材料(P0.05);(3)血小板黏附實驗:接種LLC-PK1細胞、ECV304細胞通透二氧化鈦納米管薄膜的與聚氨酯材料相比差異無顯著性意義(P0.05);(4)結果表明:二氧化鈦納米管生物透析膜具有良好的血液相容性。
[Abstract]:Background: traditional dialysis membranes are mostly synthesized from organic polymer materials. Although these materials can meet the needs of treatment, the pore size of the materials is large, the microtubules are uneven, and proteins are easily silted up on the dialysis membranes. Objective: to analyze the blood compatibility of titanium dioxide nanotube biodialysis membrane. Methods: high strength titanium dioxide nanotube film was prepared by anodic oxidation method. TIO _ 2 nanotube array films were obtained by HF gas corrosion on the bottom of nanotubes. LLC-PK1 cells and ECV304 cells were inoculated on TIO _ 2 nanotube array films, respectively. Two kinds of titanium dioxide nanotube biodialysis membranes were obtained. The hemolysis experiments were carried out. Two kinds of titanium dioxide nanotube biodialysis membranes and polyurethane materials were immersed in saline and distilled water respectively. Dynamic Coagulation time experiment: two kinds of titanium dioxide nanotube biodialysis membranes and polyurethane materials coated with rabbit blood were mixed with calcium chloride respectively. Platelet adhesion experiment: platelet-rich plasma was dripped on two kinds of titanium dioxide nanotube biodialysis membrane and polyurethane material respectively. Results and conclusion\\\. The dynamic coagulation time of the permeable titanium dioxide nanotube film 2040 / 70 min was significantly longer than that of the polyurethane material (P0.05 / 3) platelet-adhesion test: there was no significant difference in the penetration of TiO2 nanotube film between the LLC-PK1 cells and the polyurethane material after inoculation of LLC-PK1 cells with ECV304 cells. The results showed that the titanium dioxide nanotube biodialysis membrane had good blood compatibility.
【作者單位】： 新鄉(xiāng)醫(yī)學院;
【基金】：河南省科技發(fā)展攻關項目(2014HNA0032)~~
【分類號】：R318.08

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