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  本文選題：微孔鈦　切入點：選擇性激光熔覆　出處：《安徽醫(yī)科大學學報》2017年12期

【摘要】：目的探究經(jīng)堿酸熱處理的選擇性激光熔覆微孔鈦在體外生物學活性方面是否具有優(yōu)勢。方法制備光滑組(PT組)、噴砂酸蝕組(SLA組)、親水組(ModS LA組)、選擇性激光熔覆組(SLM組)、選擇性激光熔覆-堿酸熱處理組(SLM-T組)5種不同的鈦表面,對各組試件表面人類血清白蛋白(HSA)吸附行為進行研究,在各組試件表面接種小鼠骨髓間充質(zhì)干細胞(BMSCs)進行培養(yǎng),評價不同鈦試件表面的體外生物學活性。結(jié)果在5、10、20、40、60 min時,SLM-T組對HSA具有相對良好的吸附特性。第1、3、5天,與其他3組相比,SLM組和SLM-T組表現(xiàn)出最為顯著的增殖活性,差異有統(tǒng)計學意義(P0.01),第7天,5組試件堿性磷酸酶(AKP)的表達水平差異無統(tǒng)計學意義;第14天,SLM-T組有著最為顯著的AKP表達水平,差異有統(tǒng)計學意義(P0.01),SLM組與PT組的AKP表達水平差異無統(tǒng)計學意義。結(jié)論體外實驗表明,經(jīng)堿酸熱處理的選擇性激光熔覆微孔鈦具有良好的體外生物學活性,可望促進骨結(jié)合,潛在地提升種植體植入后的初期穩(wěn)定性。
[Abstract]:Objective to investigate whether selective laser cladding of microporous titanium by alkali acid heat treatment has advantages in biological activity in vitro. Methods preparation of smooth group PT group, sandblasting acid etching group SLA group, hydrophilic group Mod S LA group, selective laser cladding method. SLM group, selective laser cladding-alkali acid heat treatment group, SLM-T group, 5 different titanium surfaces, The adsorption behavior of human serum albumin (HSA) on the surface of each group of specimens was studied, and the bone marrow mesenchymal stem cells (BMSCs) were inoculated on the surface of each group of specimens for culture. The surface biological activity of different titanium samples was evaluated in vitro. Results the SLM-T group had relatively good adsorption properties on HSA at 510 ~ 20 ~ 4060 min. On the 5th day, compared with the other three groups, the SLM-T group and SLM-T group showed the most significant proliferative activity, and compared with the other three groups, the SLM-T group showed the most significant proliferative activity, and compared with the other three groups, the SLM-T group showed the most significant proliferative activity. There was significant difference in the expression of alkaline phosphatase (AKP) in the samples of the 5th group on the 7th day, but on the 14th day in the SLM-T group, the most significant AKP expression level was found in the SLM-T group. There was no significant difference in the expression of AKP between P0.01 and PT groups. Conclusion the in vitro experiments showed that selective laser cladding of microporous titanium by alkali acid heat treatment had good biological activity in vitro and could promote bone binding. Potentially improve the initial stability of implants after implantation.
【作者單位】： 安徽醫(yī)科大學第二附屬醫(yī)院口腔科;安徽醫(yī)學高等�？茖W校口腔系;
【基金】：安徽省自然科學基金(編號:1508085MH156) 安徽省學術(shù)和技術(shù)帶頭人科研活動經(jīng)費資助項目(編號:2016H075)
【分類號】：R783.1

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本文編號：1665969