發(fā)布時(shí)間：2018-03-21 11:30

  本文選題：比較組織學(xué)　切入點(diǎn)：免疫組織化學(xué)　出處：《安徽醫(yī)科大學(xué)學(xué)報(bào)》2016年10期 　論文類型：期刊論文

【摘要】：目的比較成年裸鼠與野生型小鼠皮膚形態(tài)結(jié)構(gòu)及表皮干細(xì)胞標(biāo)志物p63、角蛋白19和β1整合素的分布和表達(dá)量的差異,為后續(xù)的皮膚組織工程學(xué)研究提供客觀的參照。方法分別取8只成年裸鼠與野生型小鼠背部皮膚,制作石蠟切片,進(jìn)行HE染色,光鏡下觀察皮膚表皮、真皮和皮膚附屬器的形態(tài)結(jié)構(gòu);免疫組織化學(xué)法檢測p63、角蛋白19和β1整合素在兩種鼠皮膚的分布和表達(dá)量。結(jié)果 HE染色低倍鏡下裸鼠表皮較野生型小鼠厚,高倍鏡下裸鼠表皮由3~4層上皮細(xì)胞組成,而野生型小鼠表皮由1~2層上皮細(xì)胞組成。裸鼠表皮基底層細(xì)胞呈立方形或者矮柱形,細(xì)胞核深染,呈卵圓形,居中,野生型小鼠表皮基底層細(xì)胞呈立方形,細(xì)胞核圓形深染。裸鼠表皮有明顯的顆粒層,而野生型小鼠表皮則無。裸鼠皮脂腺?zèng)]有野生型小鼠皮脂腺發(fā)達(dá);免疫組織化學(xué)顯示p63表達(dá)于裸鼠和野生型小鼠毛囊和表皮基底層細(xì)胞的胞核中,角蛋白19表達(dá)于裸鼠和野生型小鼠皮膚毛囊和基底層細(xì)胞中,β1整合素表達(dá)于裸鼠皮膚毛囊和表皮顆粒層細(xì)胞中及野生型小鼠毛囊中;裸鼠p63、β1整合素和角蛋白19的表達(dá)量少于野生型小鼠,差異有統(tǒng)計(jì)學(xué)意義(P0.01)。結(jié)論成年裸鼠與野生型小鼠皮膚形態(tài)結(jié)構(gòu)及表皮干細(xì)胞標(biāo)志物的分布和表達(dá)量存在差異,可以作為區(qū)別裸鼠與野生型小鼠皮膚的參考。
[Abstract]:Objective to compare the distribution and expression of epidermal stem cell markers p63, keratin 19 and 尾 1 integrin in adult nude mice and wild type mice. Methods the back skin of 8 adult nude mice and wild-type mice were taken to make paraffin sections and stained with HE. The skin epidermis was observed under light microscope. The distribution and expression of p63, keratin 19 and 尾 1 integrin in the skin of mice were detected by immunohistochemistry. Results the epidermis of nude mice was thicker than that of wild-type mice under HE staining. The epidermis of nude mice was composed of three or four layers of epithelial cells under high power microscope, while the epidermis of wild type mice was composed of 1 layer and 2 layers of epithelial cells. The basal layer of epidermis of nude mice was square or short columnar, and the nucleus was deep stained, oval and intermediate. The basal layer cells of wild mouse epidermis were square, and the nuclei were round and deep stained. The epidermis of nude mice had obvious granular layer, but the epidermis of wild type mice was not. The sebaceous glands of nude mice were not as developed as those of wild type mice. Immunohistochemical staining showed that p63 was expressed in the nuclei of hair follicles and epidermal basal layer cells in nude mice and wild type mice. Keratin 19 was expressed in hair follicles and basal layer cells of nude mice and wild type mice, and 尾 1 integrin was expressed in hair follicles and granular layer cells of skin of nude mice and in hair follicles of wild-type mice. The expression of p63, 尾 1 integrin and keratin 19 in nude mice was lower than that in wild type mice, and the difference was statistically significant (P 0.01). Conclusion there are differences in skin morphology, distribution and expression of epidermal stem cell markers between adult nude mice and wild type mice. It can be used as a reference to distinguish the skin between nude mice and wild mice.
【作者單位】： 安徽醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院組織胚胎學(xué)教研室;
【基金】：安徽省教育廳基金項(xiàng)目(編號(hào):KJ2007A034)
【分類號(hào)】：R318
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本文編號(hào)：1643656