本文選題：殼聚糖　切入點(diǎn)：聚乙二醇　出處：《西南交通大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：冠心病作為最常見的心血管疾病之一,已嚴(yán)重威脅到人類的健康。目前,臨床上最有效的方法是介入治療法,即在病變處植入血管支架,使原本已堵塞的血管暢通。但是,支架在植入及撐開時(shí)容易造成血管壁損傷、導(dǎo)致內(nèi)膜層受損,從而引發(fā)再狹窄和血栓等并發(fā)癥。近期國(guó)內(nèi)外學(xué)者主要研究是,在支架表面進(jìn)行生物功能化構(gòu)建,使支架植入人體后可以快速形成一層內(nèi)皮,加速損傷內(nèi)皮層的修復(fù)和重建,從而解決術(shù)后并發(fā)癥問(wèn)題。本論文通過(guò)在金屬鈦箔表面沉積多巴胺涂層,并沉積巰基殼聚糖/聚乙二醇二丙烯酸酯復(fù)合水凝膠。通過(guò)反應(yīng)條件、交聯(lián)劑的含量、單體的濃度進(jìn)行水凝膠合成方法篩選;通過(guò)一系列表征評(píng)價(jià)水凝膠的形貌學(xué)特征、結(jié)構(gòu)學(xué)特征、溶脹性能、降解性能、內(nèi)皮細(xì)胞相容性能。本論文進(jìn)行單體的合成反應(yīng)及表征評(píng)價(jià),巰基殼聚糖的合成實(shí)驗(yàn)中顯示,改變巰基乙酸與殼聚糖的配比、反應(yīng)時(shí)間及碳化二亞胺的用量可以控制巰基殼聚糖的巰基度,本實(shí)驗(yàn)中篩選出的巰基化殼聚糖的反應(yīng)條件為:反應(yīng)體系pH=5.0,EDC濃度0.3mol/L,巰基乙酸與殼聚糖的物料比(質(zhì)量比)為1:1,反應(yīng)時(shí)間為10h;聚乙二醇二丙烯酸酯合成實(shí)驗(yàn)中顯示,在三乙胺和氬氣保護(hù)體系下進(jìn)行丙烯酰氯及聚乙二醇的合成反應(yīng),經(jīng)過(guò)紅外檢測(cè)及核磁共振氫譜檢測(cè)證實(shí)產(chǎn)物合成成功。本論文進(jìn)行水凝膠合成條件篩選,通過(guò)反應(yīng)條件、單體濃度、交聯(lián)劑用量等方面進(jìn)行篩選,并通過(guò)成膠條件篩選出TCS-PEGDA復(fù)合水凝膠反應(yīng)條件如下:巰基殼聚糖(TCS)濃度為:20mg/mL,聚乙二醇二丙烯酸酯(PEGDA)濃度為:100mg/mL,過(guò)硫酸銨(APS)濃度為:20mg/mL,四甲基乙二胺(TMEDA)用量為20μL。本論文對(duì)TCS-PEGDA(TP)復(fù)合水凝膠進(jìn)行性質(zhì)與特性表征,水凝膠形貌學(xué)實(shí)驗(yàn)證實(shí)隨著PEGDA含量增加,凝膠內(nèi)孔隙率越低,材料越致密;水凝膠FTIR實(shí)驗(yàn)證實(shí)合成水凝膠存在特征基團(tuán),實(shí)驗(yàn)成功制備TP水凝膠;水凝膠溶脹性能實(shí)驗(yàn)證實(shí)隨著PEGDA含量增加,水凝膠溶脹性能降低;水凝膠降解性能實(shí)驗(yàn)證實(shí)隨著PEGDA含量增加,水凝膠降解速率逐漸平緩,且TP2樣品組降解速率明顯高于其他三組;內(nèi)皮細(xì)胞黏附實(shí)驗(yàn)表明水凝膠具有良好細(xì)胞相容性,隨著巰基殼聚糖增加,細(xì)胞黏附性能越好;HE染色實(shí)驗(yàn)表明細(xì)胞可以從凝膠表面遷移到凝膠內(nèi)部。綜上所述,本論文成功合成巰基殼聚糖與聚乙二醇二丙烯酸酯復(fù)合水凝膠,并對(duì)形貌學(xué)特征、結(jié)構(gòu)學(xué)特征、溶脹性能、降解性能、內(nèi)皮細(xì)胞相容性能進(jìn)行評(píng)價(jià),最后得出TP4復(fù)合水凝膠組具有較好的溶脹性能、降解性能和內(nèi)皮細(xì)胞相容性。
[Abstract]:Coronary heart disease, as one of the most common cardiovascular diseases, has become a serious threat to human health. At present, the most effective method in clinic is interventional therapy, that is, the placement of vascular stents in the diseased areas to smooth the previously blocked blood vessels. However, Stent is easy to cause vascular wall injury when it is implanted and opened, resulting in damage of intimal layer, which leads to complications such as restenosis and thrombus. Recently, researchers at home and abroad have mainly studied the biofunctional construction on the surface of stent. The stent can quickly form a layer of endothelium after implantation, and accelerate the repair and reconstruction of the injured endodermis, thus solving the problem of postoperative complications. In this paper, dopamine coating was deposited on the surface of titanium foil. Mercapto chitosan / polyethylene glycol diacrylate composite hydrogels were deposited. The hydrogel synthesis methods were screened by reaction conditions, crosslinking agent content and monomer concentration. The morphologic characteristics of hydrogels were evaluated by a series of characterization. In this paper, the monomer synthesis reaction and characterization evaluation showed that the ratio of thioglycolic acid and chitosan was changed. The reaction time and the amount of carbodiimide can control the sulfhydryl degree of mercapto chitosan. The reaction conditions of mercapto chitosan were as follows: the concentration of EDC was 0.3 mol / L, the ratio of thioglycolic acid to chitosan was 1: 1, the reaction time was 10 h, and the synthetic experiment of polyethylene glycol diacrylate showed that the concentration of EDC was 0.3 mol / L, the ratio of mercaptoacetic acid to chitosan was 1: 1 and the reaction time was 10 h. The synthesis of acryloyl chloride and polyethylene glycol in the system of triethylamine and argon was confirmed by IR and NMR. In this paper, the conditions of hydrogel synthesis were screened, and the reaction conditions were obtained. The concentration of monomer and the amount of crosslinking agent were selected. The reaction conditions were as follows: the concentration of thioglycol-chitosan was 20 mg / mL, the concentration of polyethylene glycol diacrylate was 100 mg / mL, the concentration of ammonium persulfate was 20 mg / mL, and the dosage of tetramethyl ethylenediamine was 20 渭 L. the reaction conditions were as follows: the concentration of mercaptochitosan was 20 mg / mL, the concentration of polyethylene glycol diacrylate was 100 mg / mL, the concentration of ammonium persulfate was 20 mg / mL, and the dosage of tetramethyl ethylenediamine was 20 渭 L. The properties and properties of TCS-PEGDA-TP-based hydrogels were characterized. Hydrogel morphology experiment confirmed that with the increase of PEGDA content, the lower the porosity of the gel, the denser the material, the more dense the hydrogel was, the more characteristic groups existed in the synthetic hydrogel, and the TP hydrogel was successfully prepared by the hydrogel FTIR experiment. The swelling property of hydrogel decreased with the increase of PEGDA content, and the degradation rate of hydrogel decreased with the increase of PEGDA content. The degradation rate of TP2 sample group was significantly higher than that of the other three groups, and the endothelial cell adhesion test showed that the hydrogel had good cytocompatibility, and with the increase of mercapto chitosan, The better the cell adhesion was, the better the HE staining showed that cells could migrate from the surface of the gel to the inside of the gel. In summary, the mercapto chitosan and polyethylene glycol diacrylate hydrogels were synthesized successfully, and the morphologic characteristics of the hydrogels were analyzed. The structural characteristics, swelling properties, degradation properties and endothelial cell compatibility were evaluated. Finally, the results showed that the TP4 composite hydrogels had better swelling, degradation and endothelial cell compatibility.
【學(xué)位授予單位】：西南交通大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R318.08

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