本文選題：骨形態(tài)發(fā)生蛋白質(zhì)類　切入點(diǎn)：假體和植入物　出處：《中國組織工程研究》2016年25期 　論文類型：期刊論文

【摘要】：背景:將骨形態(tài)發(fā)生蛋白與中空多孔鈦合金復(fù)合,可提升材料與周圍骨組織的親和力。目的:觀察骨形態(tài)發(fā)生蛋白2對中空多孔金屬假體支架內(nèi)骨髓間充質(zhì)干細(xì)胞生長及成骨分化的影響。方法:將第3代SD大鼠骨髓間充質(zhì)干細(xì)胞直接接種于中空多孔金屬假體支架上,分別以含0,0.001,0.01,0.06,0.1 g/L骨形態(tài)發(fā)生蛋白2的DMEM培養(yǎng)基培養(yǎng),培養(yǎng)0,6,12,24,48 h,采用MTT法檢測細(xì)胞黏附情況;培養(yǎng)18 d,茜素紅染色檢測細(xì)胞成骨分化能力。將Transwell培養(yǎng)池置于中空多孔金屬假體支架上,上室加入5×108 L-1的骨髓間充質(zhì)干細(xì)胞懸液,下室分別加入含0,0.001,0.01,0.06,0.1 g/L骨形態(tài)發(fā)生蛋白2的DMEM培養(yǎng)基,培養(yǎng)0,6,12,24,48 h后檢測細(xì)胞遷徙能力。結(jié)果與結(jié)論:1培養(yǎng)6-48 h,不同質(zhì)量濃度骨形態(tài)發(fā)生蛋白2呈時(shí)間依賴性促進(jìn)骨髓間充質(zhì)干細(xì)胞的黏附;2培養(yǎng)18 d,經(jīng)不同質(zhì)量濃度骨形態(tài)發(fā)生蛋白2干預(yù)后,骨髓間充質(zhì)干細(xì)胞由梭形改變?yōu)槎嘟切?細(xì)胞呈多層性、重疊性排列,形成大量鈣化結(jié)節(jié),茜素紅染色呈鮮紅色;3培養(yǎng)6-48 h內(nèi),骨形態(tài)發(fā)生蛋白2可促進(jìn)骨髓間充質(zhì)干細(xì)胞的遷徙,且呈濃度、時(shí)間依賴性;4結(jié)果表明:骨形態(tài)發(fā)生蛋白2可增強(qiáng)中空多孔金屬假體支架上骨髓間充質(zhì)干細(xì)胞在的黏附能力、成骨分化能力與遷徙能力。
[Abstract]:Background: bone morphogenetic protein (BMP) was combined with hollow porous titanium alloy. To observe the effect of bone morphogenetic protein-2 on the growth and osteogenic differentiation of bone marrow mesenchymal stem cells in hollow porous metal prosthesis scaffolds. Bone marrow mesenchymal stem cells were directly inoculated on hollow porous metal prosthesis scaffolds. The cell adhesion was detected by MTT method in DMEM medium containing 0 0. 001G / L bone morphogenetic protein 2 (0. 001G / L bone morphogenetic protein 2) for 48 h. After cultured for 18 days, alizarin red staining was used to detect the osteogenic differentiation ability of the cells. The Transwell culture cell was placed on a hollow porous metal prosthesis scaffold, and 5 脳 10 8 L ~ (-1) of bone marrow mesenchymal stem cells suspension was added to the upper chamber. DMEM medium containing 0 0. 001G / L bone morphogenetic protein 2 (BMP 2) was added to the lower chamber. Results and conclusion different concentrations of bone morphogenetic protein 2 promoted bone marrow mesenchymal stem cell adhesion to bone marrow mesenchymal stem cells in a time-dependent manner for 18 days, and were cultured with different bone mass concentrations for 6 to 48 hours. After the intervention of morphogenetic protein-2, Bone marrow mesenchymal stem cells changed from fusiform to polygonal, the cells were multilayered, overlapping, and formed a large number of calcified nodules. Alizarin red staining showed bright red for 6-48 hours. Bone morphogenetic protein-2 could promote the migration of bone marrow mesenchymal stem cells in a concentration dependent manner. The results showed that bone morphogenetic protein 2 could enhance the adhesion of bone marrow mesenchymal stem cells on hollow porous metal prosthesis scaffolds. Osteogenic differentiation ability and migration ability.
【作者單位】： 青海大學(xué)附屬醫(yī)院創(chuàng)傷骨科;
【分類號】：R318.08;R687

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