本文選題：骨組織工程　切入點：快速成形　出處：《新疆醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討提高包芯骨組織工程仿生支架的親水性及體外細(xì)胞相容性的方法，為體內(nèi)實驗提供研究依據(jù)。方法：采用復(fù)合噴射低溫沉積快速成形工藝（MSLDM）制備快速成形支架，以明膠改性的PLGA／β-TCP支架（包芯骨支架）作為實驗組，未經(jīng)明膠修飾的PLGA／β-TCP支架（原始支架）作為對照組，檢測兩組支架的孔隙率、力學(xué)性能、支架表面元素分析、親水性、降解率、蛋白吸附能力；將MSCs接種于包芯骨支架和原始支架，分別測定檢測支架材料的體外細(xì)胞相容性（包括細(xì)胞黏附率、細(xì)胞增殖率，掃描電鏡觀察細(xì)胞黏附形態(tài)）。結(jié)果：兩組支架大體觀察均形成三級孔隙結(jié)構(gòu)；包芯骨支架孔隙率為85.71%±2.05%，原始支架孔隙率為82.81%±0.9%，，（P0.05）；能譜分析包芯支架表面的氮元素重量百分比為7.31%、原子百分比為8.39%；包芯骨支架的壓縮模量和抗壓強度優(yōu)于原始支架（P＜0.05）；包芯骨支架的親水率在1h和168h均高于原始支架（P＜0.05）；兩組支架的體外降解率沒有明顯差異（P＞0.05）；包芯骨支架蛋白吸附能力明顯高于原始支架（P＜0.05）；細(xì)胞在接種12h和24h時，原始支架的細(xì)胞粘附率明顯少于包芯骨支架（P＜0.05）；包芯骨支架在培養(yǎng)6d、8d、10d時的細(xì)胞增殖率明顯高于原始支架（P＜0.05）；SEM計數(shù)MSCs粘附于支架表面，包芯骨支架6d、8d、10d的細(xì)胞數(shù)量均多于原始支架（P＜0.05）。結(jié)論：經(jīng)明膠改性的PLGA／β-TCP支架親水性得到改進、體外降解性不受影響，且具有良好的細(xì)胞相容性，可用于骨組織工程支架修復(fù)骨缺損的體內(nèi)研究，具有潛在臨床應(yīng)用價值。
[Abstract]:Objective: to explore a method to improve the hydrophilicity and in vitro cytocompatibility of scaffolds for bone tissue engineering. Methods: rapid prototyping scaffolds were prepared by composite spray low temperature deposition rapid prototyping (MSLDM). The PLGA/ 尾 -TCP scaffold modified by gelatin was used as experimental group and PLGA/ 尾 -TCP scaffold without gelatin modified as control group. The porosity, mechanical properties, surface element analysis, hydrophilicity and degradation rate of the two groups were measured. MSCs was inoculated into the core-bone scaffold and the original scaffold, and the cell compatibility (including cell adhesion rate, cell proliferation rate) of the scaffold was measured. Results: the morphology of cell adhesion was observed by scanning electron microscope. The porosity of the core-bone scaffold was 85.71% 鹵2.05, the porosity of the original scaffold was 82.81% 鹵0.9and that of the original scaffold was 82.81% 鹵0.90.The weight percentage of nitrogen on the surface of the core scaffold was 7.31 and the atomic percentage was 8.390.The compression modulus and compressive strength of the core-bone scaffold were better than those of the original scaffold (P < 0.05). The hydrophilic rate of core bone scaffold was higher at 1 h and 168 h than that of original scaffold (P < 0.05), there was no significant difference in in vitro degradation rate between the two groups (P > 0.05), the protein adsorption ability of core bone scaffold was significantly higher than that of the original scaffold (P < 0.05), and the cells were inoculated at 12 h and 24 h after inoculation. The cell adhesion rate of the original scaffold was significantly lower than that of the core-bone scaffold (P < 0.05), and the cell proliferation rate of the core-bone scaffold was significantly higher than that of the original scaffold (P < 0.05) when cultured for 6 days or 8 days or 10 days, and the number of MSCs adhered to the surface of the scaffold was significantly higher than that of the original scaffold (P < 0.05). The number of cells in the scaffold was more than that in the original scaffold (P < 0.05). Conclusion: the hydrophilicity of PLGA/ 尾 -TCP scaffold modified by gelatin has been improved, the biodegradability of the scaffold is not affected in vitro, and it has good cytocompatibility. It can be used to repair bone defect with bone tissue engineering scaffold in vivo, and has potential clinical application value.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R318.08

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