本文關(guān)鍵詞： 骨組織工程 β-磷酸三鈣 鎵 生物相容性 細(xì)胞毒性　出處：《長(zhǎng)江大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：骨骼是人體內(nèi)最堅(jiān)硬的器官,對(duì)人們正常的生活有重要作用,一旦出現(xiàn)骨損傷,會(huì)對(duì)人體會(huì)造成巨大的傷害。近年來,骨損傷患者越來越多,臨床上目前常用的治療方法是進(jìn)行骨修復(fù)材料填充。無機(jī)生物陶瓷材料中的β-磷酸三鈣的組成與人體內(nèi)骨的無機(jī)鹽成分相似,在人體內(nèi)有良好的生物相容性及生物降解性,降解后產(chǎn)生的鈣、磷等元素可被人體吸收用于骨的重建,是目前骨修復(fù)領(lǐng)域研究的重點(diǎn)方向之一。為了更進(jìn)一步提高β-磷酸三鈣在骨組織修復(fù)過程的性能,可以通過離子摻雜法來改變其理化性質(zhì)和生物學(xué)性能。人體中很多微量元素具有重要的生化作用。鎵作為人體內(nèi)非必需微量元素,能夠抑制破骨細(xì)胞再吸收,抑制骨溶解,阻止骨鈣釋放,改變骨中Ⅰ型膠原和纖維蛋白的基因表達(dá),有利于新骨的形成,還能增加骨骼中鈣和磷的含量,直接作用于人體骨的形成。因此本課題提出把鎵摻雜進(jìn)β-磷酸三鈣中,進(jìn)一步改善和提高其生物活性,并系統(tǒng)研究其相關(guān)性能的變化。本試驗(yàn)采用濕法工藝法按(Ca+Ga)/P=1.5的比例合成0%、5%、10%鎵摻雜β-磷酸三鈣粉體。XRD分析表明,鎵摻雜β-磷酸三鈣多孔骨修復(fù)材料保持純凈且結(jié)晶度較好。鎵的引入導(dǎo)致β-磷酸三鈣晶格收縮,鎵摻雜量越大,晶體體積收縮越明顯。傅里葉紅外光譜分析表明,隨著鎵離子摻入量的增加,β-磷酸三鈣的特征峰強(qiáng)度逐漸減弱。電鏡形貌分析表明,鎵摻雜β-磷酸三鈣多孔骨修復(fù)材料內(nèi)部有三維網(wǎng)狀結(jié)構(gòu)的大孔與貫通性良好的微孔結(jié)構(gòu),有利于骨組織的修復(fù),符合骨替代材料的要求。在制備鎵摻雜β-磷酸三鈣骨修復(fù)材料時(shí),選擇濃度為5 wt%的聚乙烯醇溶液作為粘結(jié)劑,25 wt%硬脂酸為致孔劑,可使得鎵摻雜β-磷酸三鈣多孔骨修復(fù)材料在機(jī)械強(qiáng)度和孔隙率之間達(dá)到最優(yōu),并且無雜質(zhì)引入。在模擬體液中對(duì)鎵摻雜β-磷酸三鈣骨修復(fù)材料進(jìn)行降解試驗(yàn),經(jīng)X射線衍射及傅里葉紅外分析可知摻鎵β-磷酸三鈣骨修復(fù)材料表面有類骨磷灰石物質(zhì)沉積,隨著鎵摻雜量增加,新生成物質(zhì)的結(jié)晶度有一定增強(qiáng)。利用MTT法進(jìn)行細(xì)胞增殖試驗(yàn),結(jié)果表明摻鎵β-磷酸三鈣骨修復(fù)材料無細(xì)胞毒性,對(duì)于細(xì)胞的增殖有一定促進(jìn)作用。堿性磷酸酶測(cè)定表明試驗(yàn)摻鎵β-磷酸三鈣骨修復(fù)材料能促進(jìn)成骨細(xì)胞中ALP的分泌。根據(jù)一系列試驗(yàn)結(jié)果可知,摻鎵β-磷酸三鈣骨修復(fù)材料有較好的可降解性及生物相容性,是一種有前景的骨修復(fù)材料。
[Abstract]:Bone is the hardest organ in the human body and plays an important role in people's normal life. Once bone injury occurs, it will cause great harm to human body. In recent years, more and more patients with bone injury are suffering from bone injury. At present, the commonly used treatment method is bone repair material filling. The composition of 尾 -tricalcium phosphate in inorganic bioceramics is similar to the inorganic salt composition of human bone, and has good biocompatibility and biodegradability in human body. Calcium, phosphorus and other elements produced by degradation can be absorbed by the human body for bone reconstruction, which is one of the important research directions in the field of bone repair. In order to further improve the performance of 尾 -tricalcium phosphate in bone tissue repair, The physical and chemical properties and biological properties can be changed by ion doping. Many trace elements in the human body have important biochemical effects. Gallium, as a non-essential trace element in human body, can inhibit the reabsorption of osteoclasts and inhibit osteolysis. Blocking the release of bone calcium, altering the gene expression of type I collagen and fibrin in bone, which is beneficial to the formation of new bone, can also increase the contents of calcium and phosphorus in bone. Therefore, this paper proposes that doping gallium into 尾 -tricalcium phosphate can further improve and enhance its biological activity. In this experiment, the method of wet method was used to synthesize 0% 10% gallium doped 尾 -tricalcium phosphate powder according to the ratio of Ca Ga)/P=1.5, and the results showed that the content of Ga-doped 尾 -tricalcium phosphate powder was better than that of 尾 -tricalcium phosphate powder. Gallium doped 尾 -tricalcium phosphate porous bone repair materials keep pure and good crystallinity. The introduction of gallium leads to the contraction of 尾 -tricalcium phosphate lattice, the larger the doping amount of gallium, the more obvious the shrinkage of crystal volume. The characteristic peak intensity of 尾 -tricalcium phosphate gradually decreased with the increase of gallium ion incorporation. Electron microscope analysis showed that there were macropores with three-dimensional reticular structure and micropore structure with good transfixibility in Gallium doped 尾 -tricalcium phosphate porous bone repair materials. When preparing Gallium doped 尾 -tricalcium phosphate bone repair material, polyvinyl alcohol solution with concentration of 5 wt% was selected as binder for 25 wt% stearic acid as pore-forming agent. Gallium doped 尾 -tricalcium phosphate porous bone repair material is optimized between mechanical strength and porosity, and no impurity is introduced. The degradation test of gallium doped 尾 -tricalcium phosphate bone repair material is carried out in simulated body fluid. X-ray diffraction and Fourier transform infrared analysis showed that bone-like apatite was deposited on the surface of Gallium-doped 尾 -tricalcium phosphate bone repair material, and with the increase of doping amount of gallium, The crystallinity of the newly formed material was enhanced to some extent. Cell proliferation test was carried out by MTT method. The results showed that there was no cytotoxicity in the Gallium 尾 -tricalcium phosphate repair material. Alkaline phosphatase assay showed that Gallium 尾 -tricalcium phosphate could promote the secretion of ALP in osteoblasts. Gallium 尾 -tricalcium phosphate is a promising bone repair material with good biocompatibility and biodegradability.
【學(xué)位授予單位】：長(zhǎng)江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R318.08

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