本文關(guān)鍵詞： 藻酸鹽 間質(zhì)干細(xì)胞 組織工程 生物材料 骨生物材料 海藻酸鈉 間充質(zhì)干細(xì)胞 組織工程骨 肋骨大段缺損　出處：《中國(guó)組織工程研究》2017年02期 　論文類型：期刊論文

【摘要】：背景:組織工程生物材料具有與自體組織相似的結(jié)構(gòu)與功能。目的:觀察骨生物材料復(fù)合大鼠骨髓間充質(zhì)干細(xì)胞異位成骨治療肋骨大段缺損的效果。方法:取Wistar大鼠50只,制備右側(cè)大段肋骨缺損模型,隨機(jī)分為2組,對(duì)照組于骨缺損處置入氯化鈣-藻酸鈉凝膠,實(shí)驗(yàn)組于骨缺損處植入氯化鈣-藻酸鈉-骨髓間充質(zhì)干細(xì)胞復(fù)合物。植入后2,4,8周,進(jìn)行胸部X射線及骨缺損處組織形態(tài)學(xué)觀察。結(jié)果與結(jié)論:1X射線觀察結(jié)果:植入后2周,實(shí)驗(yàn)組骨缺損區(qū)無明顯變化;植入后4周,缺損部位開始出現(xiàn)骨質(zhì)痕跡;植入后8周,缺損部位大部分充滿骨質(zhì),缺損兩端逐漸相接。對(duì)照組缺損部位始終未見明顯骨質(zhì)修復(fù),缺損兩端逐漸封閉并硬化;2骨缺損處組織形態(tài)學(xué)觀察結(jié)果:植入后2周,實(shí)驗(yàn)組材料腔隙中可見少量骨纖維組織,大量炎性細(xì)胞聚集,材料與骨質(zhì)末端無連接;對(duì)照組骨缺部位可見大量炎性細(xì)胞,未見骨組織形成。植入后4周,實(shí)驗(yàn)組支架逐漸降解,可見大量新生骨質(zhì);對(duì)照組支架僅少量降解,缺損兩端出現(xiàn)骨組織聚集。植入后8周,實(shí)驗(yàn)組支架大部分降解,可見大量骨組織、骨小梁等組織結(jié)構(gòu),缺損兩端與再生骨質(zhì)連接;對(duì)照組支架大部分降解,缺損兩端出現(xiàn)骨硬化;3結(jié)果表明:骨生物材料復(fù)合大鼠骨髓間充質(zhì)干細(xì)胞可促進(jìn)肋骨大段缺損的修復(fù)。
[Abstract]:Background: tissue engineering biomaterials have the same structure and function as autologous tissues. Objective: to observe the effect of bone biomaterials combined with bone marrow mesenchymal stem cells (BMSCs) on heterotopic osteogenesis of rib defects. 50 Wistar rats were selected. The right large rib defect model was made and divided into two groups randomly. The control group was treated with calcium chloride sodium alginate gel in the treatment of bone defect. The experimental group was implanted with calcium chloride-sodium alginate-bone marrow mesenchymal stem cell complex at the site of bone defect. Results and conclusion the results showed that there was no significant change in bone defect area in the experimental group 2 weeks after implantation. 4 weeks after implantation, bone marks began to appear in the defect. At 8 weeks after implantation, most of the defects were filled with bone, and the two ends of the defects were gradually connected with each other. In the control group, there was no obvious bone repair at the defect site, and the two ends of the defect were gradually closed and sclerotic. 2Histomorphologic observation of bone defect: at 2 weeks after implantation, a small amount of bone fiber tissue was found in the space of the experimental group, and a large number of inflammatory cells gathered, and there was no connection between the material and the end of the bone. In the control group, a large number of inflammatory cells were found in the bone defect, but no bone tissue was formed. 4 weeks after implantation, the scaffolds in the experimental group were gradually degraded and a large number of new bone was observed. In the control group, there was only a small amount of degradation and bone tissue aggregation at the two ends of the defect. 8 weeks after implantation, most of the scaffolds in the experimental group were degraded, a large number of bone tissues and trabeculae were found, and the two ends of the defect were connected with the regenerated bone. In the control group, most of the scaffolds degraded and bone sclerosis appeared at both ends of the defect. The results showed that bone biomaterials combined with bone marrow mesenchymal stem cells could promote the repair of rib defect.
【作者單位】： 鄭州大學(xué)附屬醫(yī)院/南陽市中心醫(yī)院胸外科;鄭州大學(xué)附屬醫(yī)院/南陽市中心醫(yī)院特需病房一病區(qū);北京大學(xué)附屬深圳醫(yī)院胸外科;
【基金】：深圳市科技計(jì)劃項(xiàng)目(JCYJ20140415162338820) 深圳市醫(yī)療衛(wèi)生科研項(xiàng)目(201302068)~~
【分類號(hào)】：R318.08;R687
【正文快照】： 文章快速閱讀:文題釋義:海藻酸鹽:是目前生物材料中一類研究較為熱點(diǎn)的無機(jī)物,具有可塑性強(qiáng)、可降解、組織低排異度及X射線透視不顯影的優(yōu)勢(shì),因此選取海藻酸鹽作為此次實(shí)驗(yàn)的主要生物無活性組織。為探究改善目前治療大段肋骨缺損的手段,實(shí)驗(yàn)擬采取大鼠骨髓間充質(zhì)干細(xì)胞為種子

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳寶林;王東安;;用于心血管醫(yī)療裝置的聚合物材料表面構(gòu)建與生物相容性評(píng)價(jià):聚合物生物材料表面的內(nèi)皮細(xì)胞組織工程化改性（英文）[J];中國(guó)組織工程研究;2016年30期

2 沙川華;張濤;李龍;;人體小腿肌腱生物材料力學(xué)特征實(shí)驗(yàn)研究[J];成都體育學(xué)院學(xué)報(bào);2016年04期

3 王貴玲;仇麗鴻;龐希寧;;間充質(zhì)干細(xì)胞的旁分泌作用及其在顱頜面骨再生治療中的應(yīng)用研究進(jìn)展[J];中國(guó)實(shí)用口腔科雜志;2016年06期

4 義勇;魏壘;;基質(zhì)細(xì)胞衍生因子-1與骨再生修復(fù)[J];國(guó)際骨科學(xué)雜志;2016年03期

5 趙立升;李俊杰;溫寧;;磷酸鈣類生物材料在口腔臨床應(yīng)用中的研究進(jìn)展[J];中華老年口腔醫(yī)學(xué)雜志;2016年03期

6 董君博;;骨形態(tài)發(fā)生蛋白、堿性成纖維細(xì)胞生長(zhǎng)因子生物材料在關(guān)節(jié)軟骨缺損修復(fù)中的生物性能[J];中國(guó)組織工程研究;2016年20期

7 Abhijit N Gurav;;Management of diabolical diabetes mellitus and periodontitis nexus: Are we doing enough?[J];World Journal of Diabetes;2016年04期

8 付冬梅;王拓;陳紅亮;孫勇;;不同生長(zhǎng)因子在骨再生修復(fù)中的作用研究進(jìn)展[J];西南國(guó)防醫(yī)藥;2016年02期

9 侯立剛;楊建義;;人工韌帶生物材料修復(fù)膝關(guān)節(jié)交叉韌帶損傷:問題及前景[J];中國(guó)組織工程研究;2016年08期

10 李銳;蔡平討;葉麗冰;張宏宇;肖健;;[PEAD:肝素:NGF]生物材料促進(jìn)大鼠坐骨神經(jīng)損傷恢復(fù)[J];中國(guó)生物工程雜志;2016年02期

相關(guān)博士學(xué)位論文 前1條

1 陳加榮;組織工程關(guān)節(jié)軟骨再生修復(fù)的在體無創(chuàng)監(jiān)測(cè)方法研究[D];第三軍醫(yī)大學(xué);2013年

【共引文獻(xiàn)】

相關(guān)期刊論文 前6條

1 任偉偉;李守宏;熊潔;張帆;管琴;;人牙周膜細(xì)胞與高糖損傷的代謝記憶效應(yīng)[J];中國(guó)組織工程研究;2017年04期

2 王俊鋼;李聰聰;毛廣顯;張潔;楊翠;;骨生物材料復(fù)合骨髓間充質(zhì)干細(xì)胞異位成骨修復(fù)肋骨大段缺損[J];中國(guó)組織工程研究;2017年02期

3 韓效林;李敬東;王婉玲;楊翠;李志英;;抗壞血酸促進(jìn)骨髓間充質(zhì)干細(xì)胞的上皮轉(zhuǎn)分化[J];中國(guó)組織工程研究;2017年01期

4 王文玲;申秀梅;王志濱;李雅娟;馮維;李梅霞;李詠梅;;CAD/CAM純鈦烤瓷冠對(duì)2型糖尿病患者牙周組織的影響[J];北京口腔醫(yī)學(xué);2016年06期

5 楊毅;易蕾;葉爾扎提·哈加合曼;特列克·坎扎勒;金格勒;;SDF-1/CXCR4信號(hào)通路參與骨形態(tài)發(fā)生蛋白2在誘導(dǎo)骨髓間充質(zhì)干細(xì)胞遷移的作用[J];中國(guó)組織工程研究;2016年41期

6 黃妍;曾昆;徐彪;;氨甲酰促紅細(xì)胞生成素對(duì)糖尿病模型大鼠冠脈微循環(huán)內(nèi)皮細(xì)胞的影響[J];中國(guó)藥房;2016年25期

相關(guān)博士學(xué)位論文 前1條

1 孫安玉;關(guān)節(jié)軟骨生物力學(xué)特性的超聲測(cè)量方法研究[D];浙江大學(xué);2015年

【二級(jí)參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 江建林;葉平;吳潤(rùn)發(fā);白彭;段志堅(jiān);黃_，

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