本文關(guān)鍵詞：結(jié)合組織學(xué)評(píng)分的牛角膜渾濁和滲透性方法研究　出處：《日用化學(xué)工業(yè)》2016年02期 　論文類(lèi)型：期刊論文

  更多相關(guān)文章： 化妝品 眼刺激 替代方法 牛角膜渾濁和滲透性實(shí)驗(yàn) 組織學(xué)

【摘要】：為了優(yōu)化替代眼刺激動(dòng)物試驗(yàn)的牛角膜渾濁和滲透性(BCOP)實(shí)驗(yàn)的方法,提高其適用范圍和預(yù)測(cè)性,選取新鮮牛眼角膜,直接暴露液體化學(xué)受試物作用10 min,后孵育2 h并用熒光素鈉處理90 min,采用濁度儀檢測(cè)角膜渾濁度,用酶標(biāo)儀檢測(cè)熒光素鈉滲透性,同時(shí)將角膜常規(guī)固定和制備組織學(xué)切片,觀察組織學(xué)病理變化并評(píng)分,基于上述3個(gè)指標(biāo)建立預(yù)測(cè)模型。結(jié)果顯示,將角膜渾濁度和滲透性2個(gè)定量指標(biāo),與角膜上皮組織、基質(zhì)組織和內(nèi)皮細(xì)胞的組織學(xué)變化評(píng)分進(jìn)行組合,可對(duì)輕微刺激到中等刺激的范圍進(jìn)行準(zhǔn)確細(xì)分。研究表明,結(jié)合組織學(xué)觀察的優(yōu)化BCOP方法可提高其預(yù)測(cè)性,適用于眼刺激機(jī)制研究和化妝品評(píng)估。
[Abstract]:In order to optimize the eye irritation test instead of animal bovine corneal opacity and permeability (BCOP) experiment method, improve the accuracy of its scope and selection of fresh bovine cornea, directly exposed to liquid chemical test for 10 min after 2 h incubation with fluorescein sodium treatment for 90 min, the turbidity meter to detect corneal opacity standard test, fluorescein sodium permeability by enzyme, while the conventional preparation and corneal histological observation, histological changes and pathological score, the above 3 indicators based on prediction model is established. The results showed that the corneal turbidity and permeability of 2 quantitative indicators, and the corneal epithelium, stroma endothelial cells and histological changes were combined, can accurately segment scope of slight irritation to moderate stimulation. The results show that the optimized BCOP method combined with histological observation can improve the predictive, suitable for eye stimulation machine System research and cosmetic evaluation.

【作者單位】： 廣東藥學(xué)院;廣東出入境檢驗(yàn)檢疫局技術(shù)中心;中山大學(xué);
【基金】：廣東省科技計(jì)劃資助項(xiàng)目(2015A030402005,2012B020316003)
【分類(lèi)號(hào)】：R318.08;TQ658
【正文快照】： 牛角膜渾濁和滲透性(BCOP)實(shí)驗(yàn)是經(jīng)過(guò)經(jīng)濟(jì)合作與發(fā)展組織(OECD)認(rèn)可的眼刺激替代方法之一,標(biāo)準(zhǔn)的實(shí)驗(yàn)方法通過(guò)2項(xiàng)定量指標(biāo)建立預(yù)測(cè)模型,即角膜渾濁度和熒光素鈉滲透性,以體外評(píng)分值(IVIS)對(duì)眼刺激性進(jìn)行預(yù)測(cè)[1]。BCOP方法對(duì)眼刺激分為3類(lèi),即無(wú)刺激性(IVIS≤3)、無(wú)法準(zhǔn)確分類(lèi)(，

本文編號(hào)：1406297