本文關(guān)鍵詞：牙科釋氟材料緩釋氟及再充氟性能的研究　出處：《第四軍醫(yī)大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 釋氟材料 氟化物涂膜 成膜基質(zhì) 氟釋放 氟再充

【摘要】：具有釋氟性和再充氟性能的牙齒充填修復(fù)材料能夠提高充填物周圍牙齒硬組織的抗脫礦、抗齲壞能力，預(yù)防或延緩充填物邊緣繼發(fā)齲的發(fā)生，延長修復(fù)體的壽命。測定、評價(jià)充填材料的釋氟性和再充氟性是研究釋氟性修復(fù)材料有效性的重要方面。 研究目的： 建立牙科材料釋氟和再充氟性能的研究測試平臺(tái)，評價(jià)自制的釋氟性復(fù)合樹脂及目前市售的釋氟性修復(fù)材料的緩釋氟性能和再充氟性能，探討釋氟性材料釋氟及再充氟的特點(diǎn)、機(jī)制及其影響因素，為研制、改進(jìn)新型釋氟性材料釋氟性能提供試驗(yàn)依據(jù)。 材料與方法： 1.常用釋氟充填材料緩釋氟及再充氟性能的體外測定 用8種充填材料（玻璃離子水門汀：Fuji IX、Fuji II LC、Fuji VII，復(fù)合體：Compoglass、Beautifil，復(fù)合樹脂：Z350、Charisma及試驗(yàn)I型釋氟性復(fù)合樹脂）分別制備直徑10mm、厚度1mm的試樣。將每個(gè)試樣浸入盛有5ml去離子水的聚乙烯小瓶中進(jìn)行浸泡，每天更換浸泡液，至第28天。取出試樣，吹干后浸入1.23％氟化泡沫中進(jìn)行4min氟再充，流水沖洗后再浸泡于5ml去離子水中，浸泡7天，每天更換浸泡液。用氟離子選擇電極測定每天的浸泡液中氟離子濃度。 2.釋氟性充填材料的表面形貌觀測及能譜分析 每種材料選取4個(gè)時(shí)間點(diǎn)（未浸水、浸水24h、浸水28天和浸水28天+充氟后24h）的試樣，常規(guī)干燥噴金后在掃描電鏡下觀測材料表面微觀結(jié)構(gòu)，并進(jìn)行表面元素能譜分析。 3.氟化物涂膜抑制釉質(zhì)脫礦的研究 選用6種氟化物涂膜，分別為Duraphat（含5%氟化鈉）及5種不同的成膜基質(zhì)（滲透型丙烯酸樹脂、珂巴樹脂、聚合松香、聚乙烯醇縮丁醛、甲基丙烯酸乙酯共聚樹脂）配制的氟化物涂膜。將新鮮拔除的牛切牙，制備成5mm×5mm×3mm大小的釉質(zhì)塊，隨機(jī)分成6組涂布6中氟涂膜，每組15個(gè)。試樣進(jìn)行pH循環(huán)7d后測定并計(jì)算脫礦前后釉質(zhì)表面熒光強(qiáng)度的變化率（△F%）和顯微硬度的變化率（%SMHC）。 4.成膜基質(zhì)對氟化物涂膜抑制釉質(zhì)脫礦深度的影響 釉質(zhì)塊分別涂布6種氟涂膜，經(jīng)過pH循環(huán)7d后，縱向切開，暴露剖面重新包埋，用硬組織切片機(jī)制備厚100m的釉質(zhì)切片，中性樹膠封片后于偏光顯微鏡下測量脫礦深度。 實(shí)驗(yàn)結(jié)果： 1.玻璃離子水門汀類充填材料表現(xiàn)出初期爆發(fā)性釋氟特點(diǎn)，浸水后3天釋氟性趨于平緩，其他材料初期爆發(fā)性釋氟不明顯。浸水28天后，3種玻璃離子水門汀的釋放值較高，復(fù)合體和復(fù)合樹脂的釋氟值較低。對所有材料進(jìn)行氟再充后24h，各材料的氟離子釋放量均顯著增加，其中釋氟增加幅度最大的是FujiVII和Fuji II LC，其次是Fuji IX、Beautifil和試驗(yàn)I型釋氟性復(fù)合樹脂，Compoglass，Z350和Charisma的釋氟增加幅度最低。氟再充后的2~3天各材料釋氟值逐漸下降，在3~4天后基本恢復(fù)到之前的水平。 2.掃描電鏡觀察發(fā)現(xiàn)各種充填材料因材料類型不同，，表面形貌各有差異，浸水28天后，均有不同程度的溶蝕現(xiàn)象，其中以Beautifil的溶解后留下的孔洞最明顯，溶蝕現(xiàn)象主要發(fā)生在填料邊緣及填料與基質(zhì)的結(jié)合處，而玻璃離子類材料和復(fù)合樹脂的表面溶解現(xiàn)象較輕微。能譜分析的結(jié)果表明玻璃離子類材料氟離子主要聚集在未反應(yīng)完全的玻璃顆粒中，復(fù)合體、Beautifil和試驗(yàn)I型釋氟性復(fù)合樹脂的氟離子主要聚集在填料中所含的玻璃粉中，復(fù)合樹脂由于自身含氟量極低，而且表面致密，溶解度小，故氟離子的釋放與再充均不明顯。 3. pH循環(huán)能夠有效地使釉質(zhì)發(fā)生脫礦，在釉質(zhì)表面應(yīng)用氟化物涂膜，能夠顯著提高牙釉質(zhì)的抗脫礦能力，以含滲透型丙烯酸樹脂的氟涂膜組的熒光損失量和顯微硬度值的下降率最小，提示其抗脫礦能力最好。 4.偏光顯微鏡下正常釉質(zhì)呈負(fù)性雙折射（透光），實(shí)驗(yàn)區(qū)域早期齲顯示以表層下脫礦為主要特征的病理改變，呈負(fù)性雙折射的表層和緊接下面呈正性雙折射(不透光)的病損體部。脫礦深度檢測發(fā)現(xiàn)，滲透型丙烯酸組的脫礦深度顯著小于其他涂膜組。 結(jié)論： （1）材料的緩釋氟性能與材料的類型密切相關(guān)，玻璃離子類材料釋氟性較強(qiáng)。 （2）材料的再充氟性能除與材料的類型有關(guān)外，還可能與材料溶解后的孔隙度及致密度有關(guān)。 （3）表面形貌觀察和能譜分析發(fā)現(xiàn)，大多數(shù)材料的氟離子釋放均主要來源于成分中玻璃顆粒的溶蝕。 （4）氟化物涂膜具有明顯的抑制釉質(zhì)早期齲損脫礦的作用，含滲透型丙烯酸樹脂的氟涂膜效果優(yōu)于其他組。
[Abstract]:With fluoride releasing and fluorine filling performance of dental filling repair materials can improve the anti demineralization filling around dental hard tissue, anti caries ability, prevent or delay the occurrence of secondary caries filling edge, extend the restoration of life. By evaluation of filling materials fluoride releasing and refilling of fluoride is study on the effectiveness of fluoride release important repair materials.
The purpose of the study is:
The establishment of dental materials and then study the fluoride release test platform of fluorine filling performance, evaluation of self-made fluoride releasing composite resin and the current commercially available fluoride releasing restorative materials and properties of slow-release fluoride fluorine filling performance, explore the characteristics of fluoride releasing materials and then release fluoride fluorine filling, factors, mechanism and its influence for the development of the new, improved fluoride releasing materials to provide the experimental basis for the fluoride release performance.
Materials and methods:
In vitro determination of fluorine and recharge performance of 1. commonly used fluorine release materials
Using 8 kinds of filling materials (glass ionomer cement: Fuji IX, Fuji II LC, Fuji VII, Compoglass, Beautifil, complex: composite resin: Z350, Charisma and test of I fluoride releasing composite resin) were prepared by the diameter of 10mm, thickness of 1mm. Each sample was immersed in Sheng soaked polyethylene vials with 5ml deionized water, soaking liquid changed every day, and twenty-eighth days. The specimen is removed, dried after immersion of 1.23% fluoride foam in 4min fluoride charge again, rinse water after soaking in 5ml deionized water for 7 days, every day. The replacement of soaking liquid fluoride concentration soaking solution was measured daily by fluorine ion selective electrode.
Surface morphology observation and energy spectrum analysis of 2. fluorinated filling materials
Each material was selected for 4 time points (unsoaked, 24h, 28 days and 28 days + 24h). After conventional drying, the microstructure of the material surface was observed under scanning electron microscope, and the surface element energy spectrum was analyzed.
Study on the inhibition of enamel demineralization by 3. fluoride coating
Using 6 kinds of fluoride varnish respectively, Duraphat (containing 5% sodium fluoride) and 5 kinds of film matrix (osmotic acrylic resin, copal resin, polymerized rosin, polyvinyl butyral, ethyl methacrylate copolymer resin) fluoride varnish prepared. The freshly extracted bovine incisors, prepared enamel pieces of 5mm * 5mm * the size of 3mm, were randomly divided into 6 groups of 6 fluorine coating film, each group of 15 samples. PH after 7d were determined before and after demineralization of enamel surface changes in fluorescence intensity ratio (F%) and the microhardness variation rate (%SMHC).
Effect of 4. film matrix on the inhibition of enamel demineralization by fluoride coating
The enamel blocks are respectively coated 6 fluorine coating, through the pH cycle 7d, longitudinal incision, exposure profile re embedding, with hard tissue slicing mechanism by 100m slice thick enamel, neutral balata in polarizing microscope measurement of demineralization depth.
Experimental results:
1. glass ionomer filling materials exhibit initial explosive fluoride release characteristics, after 3 days of fluoride releasing slowly, other material initial explosive fluoride release was not obvious. After 28 days, 3 kinds of glass ionomer cement release value is high, complex and composite resin fluoride release value is low. All the materials were fluoride rechargeable 24h after the release of fluoride materials were increased significantly, of which the largest increase of fluoride release is FujiVII Fuji and II LC, followed by Fuji IX, Beautifil and test of I fluoride releasing composite resin, Compoglass, Z350 and Charisma increased. The lowest fluoride fluoride release and then charge after 2~3 each day the material release fluoride decreased in 3~4 days before the return to the basic level.
Observation of various filling materials due to material type SEM of 2. different surface morphologies are different, soaking for 28 days, had different degrees of dissolution, the void left by the dissolution of Beautifil after the most obvious corrosion phenomenon occurred mainly in the filler filler and matrix and edge joints, and the phenomenon of surface dissolution of glass ionomer materials and composite resin is slight. Spectrum analysis results show that the glass ionomer materials fluoride ions gathered in the unreacted glass particles completely in complex fluoride Beautifil and test I fluoride releasing composite resin contained in the main gathering in the packing of glass powder, because of its containing composite resin fluorine content is extremely low, and dense surface, the solubility of small, so the release of fluoride and charge are not obvious.
The 3. pH cycle can effectively make the enamel demineralization occurred in the application of fluoride varnish, enamel surface, can significantly improve the ability of anti demineralization of enamel, with the decrease of fluorine containing osmotic acrylic resin coating group fluorescence loss and the microhardness values were the lowest, suggesting that the anti demineralization ability best.
4. normal enamel under polarizing microscope showed negative birefringence (light), the experimental area of early caries showed pathological changes in subsurface demineralization as the main feature of the negatively birefringent surface and then following positive birefringence (opaque) of the body. The detection of demineralization depth, demineralization the depth of penetration of acrylic group was significantly less than that of other film group.
Conclusion:
(1) the performance of the sustained release fluorine of the material is closely related to the type of the material, and the fluorine property of the glass ion material is stronger.
(2) the recharge performance of the material is related to the type of material, and it may be related to the porosity and density after the dissolution of the material.
(3) the surface morphology observation and the energy spectrum analysis show that the release of fluorine ions in most materials is mainly due to the dissolution of glass particles in the composition.
(4) the fluoride coating can obviously inhibit the demineralization of early enamel, and the effect of fluorine coating with osmotic acrylic resin is better than that of other groups.

【學(xué)位授予單位】：第四軍醫(yī)大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R783.1

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 郁章欣;兩種含氟材料預(yù)防托槽周圍牙釉質(zhì)脫礦的體外實(shí)驗(yàn)研究[D];南昌大學(xué);2014年

本文編號：1391236