本文關(guān)鍵詞：磷酸鈣陶瓷骨支架的激光燒結(jié)機(jī)理及工藝研究　出處：《中南大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 選擇性激光燒結(jié) 磷酸鈣 溫度場 多孔支架 性能

【摘要】：摘要：制備具有優(yōu)良的機(jī)械性能和生物學(xué)性能的骨支架已經(jīng)成為生物制造研究領(lǐng)域的熱點(diǎn)。磷酸鈣生物陶瓷因與自然骨有相似的無機(jī)成分和晶體結(jié)構(gòu),具有良好的生物相容性,能與骨形成化學(xué)鍵性結(jié)合,可誘導(dǎo)骨組織再生,但由于脆性大、韌性低以及降解速率不可控等缺點(diǎn),限制了它在骨組織工程中的應(yīng)用。本文通過添加增強(qiáng)相來改善磷酸鈣生物陶瓷骨支架的性能,重點(diǎn)研究了增強(qiáng)相對支架機(jī)械性能和生物學(xué)性能的影響規(guī)律與作用機(jī)理。主要研究工作與創(chuàng)新性成果如下： 1、利用ANSYS有限元分析軟件中的APDL參數(shù)化語言實(shí)現(xiàn)了激光高斯熱源的移動加載,分析了SLS過程中三維瞬態(tài)溫度場及其隨熱載荷移動速度的變化規(guī)律,優(yōu)化獲得理想的激光掃描速度為50-250mm/min。 2、利用SLS技術(shù)制備了三維多孔β-TCP骨支架,研究了不同激光掃描速度下所制備支架的平均晶粒大小和機(jī)械性能的關(guān)聯(lián)規(guī)律。研究了晶粒尺寸對細(xì)胞在支架上粘附性能的影響,MG63成骨樣細(xì)胞和人骨髓間充質(zhì)干細(xì)胞能夠在支架上粘附和生長,且支架的晶粒尺寸越小越有利于細(xì)胞的粘附和增殖,采用體外模擬體液(SBF)浸泡實(shí)驗(yàn)研究了支架的生物活性,支架顯示出良好的生物活性。 3、添加ZnO來提高支架的性能,研究了ZnO的含量對支架的微觀結(jié)構(gòu)、物相組成和機(jī)械性能的影響。發(fā)現(xiàn)ZnO的添加能夠提高支架的機(jī)械性能,且最佳的添加比例為2.5%。 4、研究了ZnO含量對骨支架細(xì)胞粘附性能和降解性能的影響,MG63細(xì)胞能夠在支架上很好的粘附。支架的降解速率隨著ZnO含量的增加不斷減慢,且提高了支架的生物活性。
[Abstract]:Abstract: the preparation of bone scaffolds with excellent mechanical and biological properties has become a hotspot in the field of biological manufacturing. Calcium phosphate bioceramics have similar inorganic composition and crystal structure to natural bone. It has good biocompatibility, can bind to bone to form chemical bond, and can induce bone regeneration, but because of its high brittleness, low toughness and uncontrollable degradation rate. The application of calcium phosphate bioceramics in bone tissue engineering is limited. In this paper, the performance of calcium phosphate bioceramic bone scaffold is improved by adding reinforcement phase. The effects of reinforcement on the mechanical and biological properties of scaffolds are studied. The main research and innovative results are as follows: 1. The moving loading of laser Gao Si heat source is realized by using APDL parameterized language in ANSYS finite element analysis software. The variation of 3D transient temperature field and its moving velocity with thermal load in SLS process is analyzed. The optimal laser scanning speed is 50 ~ 250 mm / min. 2. Three-dimensional porous 尾 -TCP bone scaffolds were fabricated by SLS technique. The relationship between the average grain size and the mechanical properties of the scaffolds prepared at different laser scanning speeds was studied, and the effect of grain size on the adhesion of cells on the scaffolds was studied. MG63 osteoblast-like cells and human bone marrow mesenchymal stem cells can adhere to and grow on the scaffold, and the smaller the grain size of the scaffold, the more conducive to cell adhesion and proliferation. The bioactivity of the scaffold was studied by SBF immersion in vitro, and the scaffold showed good bioactivity. The effects of ZnO content on the microstructure, phase composition and mechanical properties of the scaffolds were studied. It was found that the addition of ZnO could improve the mechanical properties of the scaffolds. The optimum addition ratio is 2.5. 4. The effect of ZnO content on the adhesion and degradation of bone scaffold cells was studied. MG63 cells could adhere to the scaffold very well, and the degradation rate of scaffold decreased with the increase of ZnO content. The bioactivity of the scaffold was improved.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R318.08

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本文編號：1358113