研究背景：二聚糖（Biglycan, BGN）是一種屬于糖蛋白類的蛋白質(zhì),由42kDa的核心蛋白（core protein）和兩個(gè)硫酸軟骨素／硫酸皮膚素（chondroitin sulfate, CS/dermatan sulfate, DS）糖胺聚糖（glycosaminoglycan, GAG）鏈組成,在皮膚組織結(jié)構(gòu)合成、空間填充和皮膚生理功能中起到重要的作用。雖然許多組織表達(dá)豐富的二聚糖,但其生物功能仍然知之甚少。二聚糖核心蛋白的合成起始于內(nèi)質(zhì)網(wǎng)（endoplasmic reticulum, ER）,并繼續(xù)轉(zhuǎn)移至高爾基體,通過各種糖基轉(zhuǎn)移酶（glycosyltransferases, GTs）的作用下合成CS/DS-GAG鏈。二聚糖具有組建細(xì)胞外基質(zhì)（extracellular matrix, ECM）的作用,靶向敲除二聚糖基因可以觀察到異常膠原纖維形態(tài)和骨質(zhì)疏松癥樣表型。分泌的二聚糖通過其核心蛋白和GAG側(cè)鏈的共同作用下參與合成ECM,特別是對(duì)Ⅰ、 Ⅱ、Ⅲ、Ⅵ型膠原蛋白和彈力蛋白的裝配起到重要的作用。最近研究發(fā)現(xiàn)二聚糖與炎癥密切相關(guān),在脂多糖（lipopolysaccharid... 

【文章來源】：延邊大學(xué)吉林省 211工程院校

【文章頁數(shù)】：89 頁

【學(xué)位級(jí)別】：博士

【文章目錄】：
中文摘要
ABSTRACT
ABBREVIATIONS
PART ONE
    1. Introduction
    2. Materials and methods
        2.1 Cell culture and UV irradiation
        2.2 Quantitative real-time reverse transcription-polymerase chain reaction（RT-PCR）
        2.3 Gene silencing with siRNA
        2.4 Overexpression of wild-type and mutant BGN proteins in primary human dermalfibroblasts
        2.5 Western blot analysis
        2.6 Statistics
    3. Results
        3.1 UV irradiation increased defectively-glycosylated BGN expression dose-dependently incultured human dermal fibroblasts
        3.2 UV-induced mRNA expression changes of xylosyltransferases（XYLTs） in cultured humandermal fibroblasts
        3.3 Downregulation of XYLT1 by siRNA transfection impairs BGN GAG synthesis incultured human dermal fibroblasts
        3.4 Another DS proteoglycan,DCN,showed a little or no decrease of its molecular weight byUV irradiation and XYLT1 siRNA transfection in cultured human dermal fibroblasts
        3.5 XYLT1 can add xylose on S42 and S47 on the core protein in cultured human dermalfibroblasts,while XYLT2 can add xylose only on S42,but not S47
    4. Discussion
    5. Conclusions
PART TWO
    1. Introduction
    2. Materials and Methods
        2.1 Cell culture and UV irradiation
        2.2 UV and LPS stimulation
        2.3 Quantitative real-time reverse transcription-polymerase chain reaction（RT-PCR）
        2.4 Gene silencing with siRNA
        2.5 Statistics
    3. Results
        3.1 Down-Regulation of BGN prevents UV-induced IL-6,IL-8 gene expression in humanfibroblasts
        3.2 Down-Regulation of TLR4 prevents UV-induced IL-6,IL-8 gene expression in humanfibroblasts
        3.3 Down-Regulation of BGN and TLR4 prevents LPS-induced IL-6,IL-8 gene expression inhuman fibroblasts
    4. Discussion
    5. Conclusions
References
綜述
    參考文獻(xiàn)
致謝



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