【摘要】：目的:觀察慢性紫外線(UV)損傷對小鼠角質形成細胞(KC)角蛋白(CK)1和CK10表達的影響。方法:將實驗小鼠隨機分為空白對照組(20只)和UV損傷組(20只),采用UV光療儀模擬日光中的長波紫外線(UVA)和中波紫外線(UVB)照射實驗小鼠。照射從最小紅斑量起始,UVB為0.07 J/cm~2,UVA為0.7 J/cm~2;每日1次,每周5 d,每周增加0.5個紅斑量,連續(xù)照射9周,UVB累計照射劑量達9.45 J/cm~2,UVA累計照射劑量達94.5 J/cm~2。通過蘇木精-伊紅(HE)染色和間苯二酚-堿性品紅染色(Weigert染色法)確定皮膚損傷,通過免疫組化方法分別對照射前(W0)、后(W9)CK1和CK10的表達進行檢測。應用配對t檢驗和獨立樣本t檢驗對相關數(shù)據(jù)進行統(tǒng)計分析。結果:空白對照組小鼠W0及W9時,CK1和CK10的表達差異均無統(tǒng)計學意義(P均0.05)。UV損傷組小鼠W0和W9時,CK1和CK10的表達差異均有統(tǒng)計學意義(P均0.05)。且UV損傷組小鼠CK1和CK10的表達呈明顯的定位改變。結論:多次UV照射能導致小鼠模型中CK1和CK10的表達明顯上調且定位異常。
[Abstract]:Aim: to observe the effect of chronic ultraviolet (UV) injury on the expression of (KC) keratin (CK) 1 and CK10 in mouse keratinocytes. Methods: the experimental mice were randomly divided into blank control group (n = 20) and UV injury group (n = 20). Long wave ultraviolet (UVA) and medium wave ultraviolet (UVB) irradiation were simulated by UV phototherapy instrument. From the beginning of the minimum erythema dose, UVB was 0.07 J 鈮，

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