尋常型銀屑病患者血清IL-23和IL-27水平的表達
發(fā)布時間：2019-06-18 14:21
【摘要】：目的:銀屑病是一種以上皮細胞增生、分化失控和大量T細胞浸潤為特征的慢性復(fù)發(fā)性炎癥性皮膚病。銀屑病的發(fā)病機理目前尚不十分清楚,受遺傳、代謝異常及神經(jīng)心理等諸多因素的影響,免疫功能異常在其發(fā)病中具有重要作用。研究顯示輔助性T細胞亞群及其細胞因子在自身免疫病的發(fā)病及治療中均具有重要的作用。白介素(interleukin,IL)為一大類主要由活化的淋巴細胞及樹突狀細胞產(chǎn)生的細胞因子,具有多種生物學(xué)作用。大量研究表明IL-12家族各成員以及同其他細胞因子之間存在著相互協(xié)同、相互拮抗的網(wǎng)絡(luò),不僅在細胞內(nèi)感染及炎癥過程中起著重要的調(diào)節(jié)作用,而且與銀屑病、多發(fā)性硬化癥、Crohn,s病等多種臨床疾病的發(fā)病密切相關(guān)。目前為止,IL-12家族共有4個成員:IL-12、IL-23、IL-27和IL-35。其中IL-12、IL-23和IL-27主要由樹突狀細胞、單核-巨噬細胞和EBV轉(zhuǎn)化的B淋巴母細胞產(chǎn)生,IL-35主要由Treg產(chǎn)生。此外,也可由EBV轉(zhuǎn)化的B細胞和胎盤產(chǎn)生。 IL-23由p19和IL-12中的p40兩個亞基組成,它們之間以二硫鍵相結(jié)合。主要由活化的單核細胞、巨噬細胞(Mφ)和樹突狀細胞(DC)產(chǎn)生。近年有關(guān)IL-23與銀屑病皮損的相關(guān)性的研究較多,且主要研究方向為銀屑病皮損處IL-23表達水平與銀屑病嚴重程度的相關(guān)性研究以及IL-23與銀屑病皮損動物模型建立的相關(guān)研究。有關(guān)銀屑病患者血清中IL-23水平與疾病的相關(guān)性研究的報道較少。IL-27由EBI3和P28兩個亞基組成,其中EBI3與IL-12的p40亞基同源, p28與IL-12的p35亞基同源。IL-27主要產(chǎn)生于抗原呈遞細胞(單核細胞、巨噬細胞、樹突狀細胞),在單核細胞衍生的樹突狀細胞、活化的單核細胞和活化的巨噬細胞中水平最高。國內(nèi)尚未見有關(guān)銀屑病患者血清中IL-27水平與疾病嚴重程度的相關(guān)性研究報道。 本實驗通過檢測尋常型銀屑病患者外周血血清中的IL-23和IL-27水平與正常對照組比較,研究血清中IL-23、IL-27表達水平與尋常型銀屑病發(fā)病的相關(guān)性及與病情發(fā)展的關(guān)系,進一步探討銀屑病的發(fā)病機制,為銀屑病的預(yù)防和治療提供新思路。 方法:病例組:尋常型銀屑病患者29例,均來自2010年6月至2010年12月河北醫(yī)科大學(xué)第二醫(yī)院皮膚科門診,符合銀屑病的診斷標(biāo)準(zhǔn)。排除紅斑狼瘡、天皰瘡、皮肌炎等自身免疫性疾病以及其它嚴重系統(tǒng)性疾病,且三個月內(nèi)未應(yīng)用過免疫抑制劑或皮質(zhì)類固醇激素等影響自身免疫功能的藥物。并根據(jù)病情將病例組分為兩組:靜止期組8例,進展期組21例。對照組:11例健康者,來自河北醫(yī)科大學(xué)第二醫(yī)院健康工作人員,均無免疫性疾病及家族史,經(jīng)t檢驗,性別、年齡與病例組相比無顯著差異。由同一工作人員對病例組患者進行PASI評分,采集對照組和病例組靜脈血各約2ml,分離血清,采用ELISA法檢測血清中IL-23和IL-27表達水平的值,所得數(shù)據(jù)用SPSS17.0軟件進行統(tǒng)計學(xué)處理,因測得的IL-23的數(shù)據(jù)滿足正態(tài)性和方差齊性,故用完全隨機設(shè)計的兩均數(shù)比較的統(tǒng)計學(xué)方法;銀屑病患者組中的進展期組和靜止期IL-23表達水平滿足正態(tài)性和方差齊性,故也可采用上述方法進行統(tǒng)計學(xué)處理。病例組和正常對照組所測得的IL-27的數(shù)據(jù)均不具有正態(tài)性,用兩個獨立樣本的非參數(shù)檢驗分析兩組間差別。檢驗水準(zhǔn)均取P0.05有統(tǒng)計學(xué)意義。如病例組和正常對照組所得結(jié)果具有顯著性差異,采用兩變量的Pearson相關(guān)分析對病例組IL-23和IL-27表達水平與相應(yīng)的PASI評分水平進行相關(guān)分析,以P0.05作為有統(tǒng)計學(xué)意義。 結(jié)果: 1血清中IL-23濃度測定結(jié)果(單位pg/ml):尋常型銀屑病組平均值為(51.556±7.081),明顯高于正常對照組(17.862±6.639) (t=13.657, P0.05),差異有統(tǒng)計學(xué)意義,病例組中進展期組與靜止期組無顯著性差異(t=0.627,P0.05)。病例組IL-23與臨床PASI評分經(jīng)過直線相關(guān)性分析有正相關(guān)關(guān)系(r=0.629,P0.05)。 2血清中IL-27濃度測定結(jié)果(單位pg/ml)):病例組與對照組的數(shù)值分布均不滿足正態(tài)性,應(yīng)用非參數(shù)檢驗,兩組無統(tǒng)計學(xué)差別。U值為128.000, P=0.336,P0.05。 結(jié)論: 1尋常型銀屑病患者血清中IL-23表達水平高于正常對照組,表明IL-23的高表達為銀屑病患者免疫異常表現(xiàn)之一,證實了尋常型銀屑病的發(fā)病與T細胞免疫失衡有關(guān)。 2尋常型銀屑病血清中IL-23的表達水平在進展期組與靜止期組無顯著差異,說明IL-23的表達水平與銀屑病的進展無明確關(guān)系,不能作為反應(yīng)疾病進展情況的指標(biāo)。 3尋常型銀屑病血清中IL-23的表達水平與相應(yīng)的PASI評分的值存在直線相關(guān)關(guān)系,且為正相關(guān),說明血清中IL-23的表達水平可以反映疾病嚴重程度。 4尋常型銀屑病患者血清中IL-27表達水平與正常對照組差異無統(tǒng)計學(xué)意義,說明IL-27的表達水平與尋常型銀屑病的發(fā)病機制可能無明確關(guān)聯(lián),有待進一步研究。
[Abstract]:Objective: Psoriasis is a chronic and recurrent inflammatory skin disease characterized by epithelial cell proliferation, out-of-control of differentiation and a large number of T-cell infiltrates. The pathogenesis of psoriasis is not very clear. It is affected by the factors such as genetic, metabolic abnormality and neuropsychology. The abnormal immune function plays an important role in the pathogenesis of psoriasis. It is shown that T-cell subsets and their cytokines play an important role in the pathogenesis and treatment of autoimmune diseases. Interleukin (IL) is a major class of cytokines produced by activated lymphocytes and dendritic cells, and has a variety of biological effects. A large number of studies have shown that each member of the IL-12 family and the other cytokines are mutually synergistic and antagonistic, not only play an important role in the internal infection and the inflammation process, but also play an important role in the treatment of psoriasis, multiple sclerosis, Crohn's disease, The incidence of multiple clinical diseases, such as s disease, is closely related. So far, there are four members of the IL-12 family: IL-12, IL-23, IL-27 and IL-35. IL-12, IL-23 and IL-27 are mainly produced by dendritic cells, mononuclear-macrophages and EBV-transformed B-lymphoblasts, and IL-35 is mainly produced by Treg. In addition, the B cells and the placenta transformed by the EBV may also be produced. IL-23 consists of p40 two subunits in p19 and IL-12, with a disulfide bond phase Binding. It is mainly composed of activated monocytes, macrophages (M-type) and dendritic cells (DC). The relationship between the level of IL-23 and the severity of psoriasis and the relationship between the expression of IL-23 and the severity of psoriasis and the relationship between IL-23 and the animal model of psoriatic lesions were studied in recent years. A study on the relationship between the level of IL-23 and the disease in the serum of patients with psoriasis Less. The IL-27 consists of two subunits of EBI3 and P28, wherein the EBI3 is homologous to the p40 subunit of IL-12, p28 and the p35 subunit of IL-12 Homologous. IL-27 is mainly produced in antigen-presenting cells (monocytes, macrophages, dendritic cells), dendritic cells derived from monocytes, activated monocytes, and activated macrophages The correlation between the level of IL-27 and the severity of the disease in the serum of patients with psoriasis has not been found in China. The relationship between the level of IL-23 and IL-27 and the relationship between the level of IL-23 and IL-27 in the serum of patients with psoriasis vulgaris and the relationship between the development of the disease and the relationship between the level of IL-23 and IL-27 in the peripheral blood of patients with psoriasis vulgaris were studied. The pathogenesis of psoriasis is the prevention and treatment of psoriasis. Methods:29 cases of patients with psoriasis vulgaris were from June 2010 to December 2010 in the Department of Dermatology of the Second Hospital of Hebei Medical University. The diagnostic criteria of psoriasis include autoimmune diseases such as lupus erythematosus, pemphigus, dermatomyositis, and other serious systemic diseases, and no such effects as immunosuppressants or corticosteroid hormones have been applied for three months. the medicine with the function of body immunity, and the case components are divided into two groups according to the condition of the disease, and 8 cases of the rest period group The control group (n = 21), the control group (n = 11), the control group (n = 11), the health staff from the second hospital of hebei medical university, no immune disease and family history, t-test, sex, age and case. The scores of IL-23 and IL-27 in the serum were measured by the same staff, and the levels of IL-23 and IL-27 in the serum were measured by ELISA. The obtained data was soft with SPSS17.0. The statistical method for the statistical treatment of the parts and the data of IL-23 as measured meet the positive and non-uniformity, so the statistical method is compared with the two-to-average number compared with the complete random design; the level of the development period and the expression level of the IL-23 in the group of patients with psoriasis can meet the normality and the homogeneity, and the above-mentioned method can be used as well. The data of IL-27 measured in both the case group and the normal control group were not positive, and the non-parametric test of the two independent samples was used. The difference between the two groups was analyzed and analyzed. The test level was the same as that of P0. The level of IL-23 and IL-27 in the case group and the corresponding PASI score were analyzed by Pearson correlation analysis of two variables, and the correlation between the level of IL-23 and IL-27 in the case group and the corresponding PASI score was analyzed by Pearson correlation analysis of the two variables. be used as Statistical significance. Results: The results of the determination of IL-23 in 1 serum (unit pg/ ml): The mean value of the normal psoriasis group was (51.556-7.081), which was significantly higher than that of the normal control group (17.862-6.639) (t = 13.657, P0.05). No significant difference ( T = 0.627, P0.05). There was a positive correlation between IL-23 and clinical PASI score in case group (t = 0.627, P0.05). R = 0.629, P0.05). The concentration of IL-27 in the serum (in pg/ ml): the numerical distribution of the case group and the control group did not meet the positive rate. No statistical difference was found between the two groups. U value was 128. 000 Conclusion: The level of IL-23 in the serum of patients with psoriasis vulgaris is higher than that of the normal control group, indicating that the high expression of IL-23 is one of the abnormal behavior of the patients with psoriasis. The expression level of IL-23 in the serum of psoriasis vulgaris was not significantly different from that in the quiescent period, and the expression level of IL-23 and the level of the T-cell immune imbalance were found. There was no clear relationship between the level of IL-23 and the value of the corresponding PASI score in the serum of psoriasis vulgaris, and the relationship between the expression level of IL-23 and the corresponding PASI score in the serum of psoriasis vulgaris was related to that of the corresponding PASI score. The expression level of IL-23 in serum of patients with psoriasis vulgaris was positively correlated to the severity of the disease. The level of IL-27 expression in the serum of patients with psoriasis vulgaris was not statistically significant than that of the normal control group, and the expression of IL-27 was described.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R758.63
本文編號：2501549
[Abstract]:Objective: Psoriasis is a chronic and recurrent inflammatory skin disease characterized by epithelial cell proliferation, out-of-control of differentiation and a large number of T-cell infiltrates. The pathogenesis of psoriasis is not very clear. It is affected by the factors such as genetic, metabolic abnormality and neuropsychology. The abnormal immune function plays an important role in the pathogenesis of psoriasis. It is shown that T-cell subsets and their cytokines play an important role in the pathogenesis and treatment of autoimmune diseases. Interleukin (IL) is a major class of cytokines produced by activated lymphocytes and dendritic cells, and has a variety of biological effects. A large number of studies have shown that each member of the IL-12 family and the other cytokines are mutually synergistic and antagonistic, not only play an important role in the internal infection and the inflammation process, but also play an important role in the treatment of psoriasis, multiple sclerosis, Crohn's disease, The incidence of multiple clinical diseases, such as s disease, is closely related. So far, there are four members of the IL-12 family: IL-12, IL-23, IL-27 and IL-35. IL-12, IL-23 and IL-27 are mainly produced by dendritic cells, mononuclear-macrophages and EBV-transformed B-lymphoblasts, and IL-35 is mainly produced by Treg. In addition, the B cells and the placenta transformed by the EBV may also be produced. IL-23 consists of p40 two subunits in p19 and IL-12, with a disulfide bond phase Binding. It is mainly composed of activated monocytes, macrophages (M-type) and dendritic cells (DC). The relationship between the level of IL-23 and the severity of psoriasis and the relationship between the expression of IL-23 and the severity of psoriasis and the relationship between IL-23 and the animal model of psoriatic lesions were studied in recent years. A study on the relationship between the level of IL-23 and the disease in the serum of patients with psoriasis Less. The IL-27 consists of two subunits of EBI3 and P28, wherein the EBI3 is homologous to the p40 subunit of IL-12, p28 and the p35 subunit of IL-12 Homologous. IL-27 is mainly produced in antigen-presenting cells (monocytes, macrophages, dendritic cells), dendritic cells derived from monocytes, activated monocytes, and activated macrophages The correlation between the level of IL-27 and the severity of the disease in the serum of patients with psoriasis has not been found in China. The relationship between the level of IL-23 and IL-27 and the relationship between the level of IL-23 and IL-27 in the serum of patients with psoriasis vulgaris and the relationship between the development of the disease and the relationship between the level of IL-23 and IL-27 in the peripheral blood of patients with psoriasis vulgaris were studied. The pathogenesis of psoriasis is the prevention and treatment of psoriasis. Methods:29 cases of patients with psoriasis vulgaris were from June 2010 to December 2010 in the Department of Dermatology of the Second Hospital of Hebei Medical University. The diagnostic criteria of psoriasis include autoimmune diseases such as lupus erythematosus, pemphigus, dermatomyositis, and other serious systemic diseases, and no such effects as immunosuppressants or corticosteroid hormones have been applied for three months. the medicine with the function of body immunity, and the case components are divided into two groups according to the condition of the disease, and 8 cases of the rest period group The control group (n = 21), the control group (n = 11), the control group (n = 11), the health staff from the second hospital of hebei medical university, no immune disease and family history, t-test, sex, age and case. The scores of IL-23 and IL-27 in the serum were measured by the same staff, and the levels of IL-23 and IL-27 in the serum were measured by ELISA. The obtained data was soft with SPSS17.0. The statistical method for the statistical treatment of the parts and the data of IL-23 as measured meet the positive and non-uniformity, so the statistical method is compared with the two-to-average number compared with the complete random design; the level of the development period and the expression level of the IL-23 in the group of patients with psoriasis can meet the normality and the homogeneity, and the above-mentioned method can be used as well. The data of IL-27 measured in both the case group and the normal control group were not positive, and the non-parametric test of the two independent samples was used. The difference between the two groups was analyzed and analyzed. The test level was the same as that of P0. The level of IL-23 and IL-27 in the case group and the corresponding PASI score were analyzed by Pearson correlation analysis of two variables, and the correlation between the level of IL-23 and IL-27 in the case group and the corresponding PASI score was analyzed by Pearson correlation analysis of the two variables. be used as Statistical significance. Results: The results of the determination of IL-23 in 1 serum (unit pg/ ml): The mean value of the normal psoriasis group was (51.556-7.081), which was significantly higher than that of the normal control group (17.862-6.639) (t = 13.657, P0.05). No significant difference ( T = 0.627, P0.05). There was a positive correlation between IL-23 and clinical PASI score in case group (t = 0.627, P0.05). R = 0.629, P0.05). The concentration of IL-27 in the serum (in pg/ ml): the numerical distribution of the case group and the control group did not meet the positive rate. No statistical difference was found between the two groups. U value was 128. 000 Conclusion: The level of IL-23 in the serum of patients with psoriasis vulgaris is higher than that of the normal control group, indicating that the high expression of IL-23 is one of the abnormal behavior of the patients with psoriasis. The expression level of IL-23 in the serum of psoriasis vulgaris was not significantly different from that in the quiescent period, and the expression level of IL-23 and the level of the T-cell immune imbalance were found. There was no clear relationship between the level of IL-23 and the value of the corresponding PASI score in the serum of psoriasis vulgaris, and the relationship between the expression level of IL-23 and the corresponding PASI score in the serum of psoriasis vulgaris was related to that of the corresponding PASI score. The expression level of IL-23 in serum of patients with psoriasis vulgaris was positively correlated to the severity of the disease. The level of IL-27 expression in the serum of patients with psoriasis vulgaris was not statistically significant than that of the normal control group, and the expression of IL-27 was described.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R758.63
【引證文獻】
相關(guān)碩士學(xué)位論文 前1條
1 聶珊;多效基因RANKL對股骨頸抗壓強度指數(shù)和體重指數(shù)的影響[D];湖南師范大學(xué);2012年
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