【摘要】：背景與目的 皮膚光老化主要指人的皮膚受紫外線等外界環(huán)境因素的影響出現(xiàn)衰老的現(xiàn)象,主要表現(xiàn)為皮膚粗糙、皺紋色斑出現(xiàn)等,不僅影響一個(gè)人的外表,而且與皮膚癌有正相關(guān)性,它的發(fā)病機(jī)制目前不完全清楚。皮膚光老化的治療方法較多,YAG激光治療是近幾年發(fā)展起來(lái)的除皺緊膚術(shù),具有創(chuàng)傷小,臨床愈合快的特點(diǎn),在臨床上治療皮膚光老化取得了不錯(cuò)的療效,但其具體治療機(jī)制目前并不清楚。本研究通過(guò)大鼠皮膚光老化動(dòng)物模型,進(jìn)一步研究皮膚光老化和YAG激光治療的機(jī)制,以期對(duì)皮膚光老化的臨床治療有所幫助。 方法 選取雄性SD大鼠40只作為研究對(duì)象,隨機(jī)選取10只作為對(duì)照組,其余30只復(fù)制皮膚光老化模型,模型復(fù)制成功后再隨機(jī)分成三組(光老化組、激光治療組、自然恢復(fù)組),每只大鼠皮膚處理部位分別做組織病理HE染色,并提取RNA,逆轉(zhuǎn)錄成cDNA,做SYBR Green Ⅰ real time PCR檢測(cè)四組大鼠皮膚中β-actin、Ⅰ型膠原、Ⅲ型膠原、CTGF、TGF-β1的mRNA表達(dá)情況。 結(jié)果 肉眼觀察：光老化組大鼠背部皮膚增厚,粗糙,出現(xiàn)較深皺紋,經(jīng)YAG激光治療后大鼠皮膚光老化癥狀減輕,而自然恢復(fù)組變化不明顯；顯微鏡觀察：光老化組大鼠皮膚HE染色,表皮紊亂,疏松增厚、厚薄不均,真皮膠原纖維斷裂、排列扭曲紊亂,有炎性細(xì)胞浸潤(rùn),YAG激光治療后,癥狀明顯改善；與對(duì)照組相比,光老化組Ⅰ型膠原、Ⅲ型膠原、CTGF、TGF-β1mRNA表達(dá)明顯降低,YAG激光治療后,這些基因mRNA表達(dá)明顯升高,激光治療組與自然恢復(fù)組相比,除CTGF外,Ⅰ型膠原、Ⅲ型膠原、TGF-p的mRNA表達(dá)均明顯升高。 結(jié)論 1320nmND:YAG激光治療大鼠皮膚光老化效果顯著,其治療機(jī)制可能與其能夠刺激皮膚中Ⅰ型膠原、Ⅲ型膠原、TGF-β1mRNA表達(dá)升高有關(guān)。
[Abstract]:Background and objective skin photoaging mainly refers to the aging phenomenon of human skin affected by ultraviolet and other external environmental factors, mainly manifested in rough skin, wrinkle spots and so on, which not only affects a person's appearance. And there is a positive correlation with skin cancer, its pathogenesis is not fully clear. There are many treatments for skin photoaging. YAG laser therapy is a wrinkle tightening technique developed in recent years, which has the characteristics of small trauma and rapid clinical healing, and has achieved good results in clinical treatment of skin photoaging. However, the specific treatment mechanism is not clear at present. In this study, the mechanism of skin photoaging and YAG laser therapy was further studied through the animal model of skin photoaging in rats, in order to be helpful to the clinical treatment of skin photoaging. Methods 40 male SD rats were randomly selected as control group and the other 30 rats as skin photoaging model. After successful replication of the model, they were randomly divided into three groups: photoaging group and laser treatment group. In the natural recovery group, pathological HE staining was performed in the skin treatment sites of each rat, and cDNA, was extracted from the skin of each rat. SYBR Green 鈪，

本文編號(hào)：2492781