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FHIT、hTERT蛋白在皮膚鱗狀細(xì)胞癌中的表達(dá)及意義

發(fā)布時(shí)間:2019-05-01 07:33
【摘要】:目的:通過檢測(cè)脆性組氨酸三聯(lián)體(FHIT)及人類端粒酶逆轉(zhuǎn)錄酶(hTERT)蛋白在皮膚鱗狀細(xì)胞癌(SCC)、鮑溫病(BD)、日光角化病(SK)及正常對(duì)照皮膚組織中的表達(dá)情況,探討FHIT及hTERT蛋白與SCC發(fā)生及演進(jìn)的關(guān)系。 方法:①收集經(jīng)病理確診的50例SCC、20例BD及15例SK的蠟塊,10例對(duì)照皮膚的蠟塊,均進(jìn)行連續(xù)切片。②采用免疫組化PV-6000法檢測(cè)上述組織中FHIT和hTERT蛋白的表達(dá)。比較SCC、SK、BD及對(duì)照皮膚之間hTERT及FHIT的表達(dá)情況。 結(jié)果:①FHIT在對(duì)照組、SK組、BD組及SCC組的表達(dá)陽性率分別為100.0%、73.3%、55.0%和44.0%,FHIT蛋白的表達(dá)在對(duì)照組和BD組、SCC組之間(X2=6.429、10.500),SK組和SCC組之間(X2=3.972),高分化組和中低分化組之間(X2=4.020),無淋巴結(jié)轉(zhuǎn)移組和有淋巴結(jié)轉(zhuǎn)移組之間(X2=4.788),差異均有顯著性(p均<0.05)。FHIT在對(duì)照組皮膚均為陰性,在SK組、BD組及SCC組的表達(dá)陽性率分別為40.0%、70.0%和80.0%,hTERT蛋白的表達(dá)在對(duì)照組和SK組、BD組和SCC組之間均有顯著性差異(p均<0.05),在SK組和SCC組之間(X2=8.924),高分化組和中低分化組之間(X2=4.861),無淋巴結(jié)轉(zhuǎn)移組和有淋巴結(jié)轉(zhuǎn)移組之間(X2=3.947),差異也均有顯著性(p均<0.05),在SK組與BD組和SCC組之間,差異均無顯著性(p均>0.05)。③在SCC組中FHIT陽性率、陽性強(qiáng)度越高,其hTERT的表達(dá)陽性率和陽性強(qiáng)度越低。相關(guān)性分析結(jié)果也表明,SCC組中FHIT蛋白的陽性表達(dá)與hTERT蛋白的陽性表達(dá)呈負(fù)相關(guān)(r=-0.363,p=0.010)。 結(jié)論: FHIT蛋白的表達(dá)減少與皮膚鱗狀細(xì)胞癌的發(fā)生及演進(jìn)有關(guān);hTERT蛋白的表達(dá)增多與皮膚鱗狀細(xì)胞癌的發(fā)生及演進(jìn)有關(guān);FHIT和hTERT在皮膚鱗狀細(xì)胞癌發(fā)生、演進(jìn)過程中發(fā)揮拮抗作用。
[Abstract]:Objective: to detect the expression of fragile histidine triad (FHIT) and human telomerase reverse transcriptase (hTERT) protein in skin squamous cell carcinoma (SCC), (SCC), Bowen's disease (BD), daylight keratosis (SK) and normal skin tissues. To investigate the relationship between FHIT and hTERT proteins and the genesis and progression of SCC. Methods: 1Wax blocks were collected from 50 cases of SCC,20, 15 cases of SK and 50 cases of BD, and 10 cases of normal skin were sectioned continuously. 2 Immunohistochemistry PV-6000 method was used to detect the expression of FHIT and hTERT protein in the above tissues. To compare the expression of hTERT and FHIT between SCC,SK,BD and control skin. Results: the positive rates of 1FHIT expression in control group, SK group, BD group and SCC group were 100. 0%, 73. 3%, 55. 0% and 44. 0%, respectively. The expression of Fhit protein was between control group and BD group and SCC group (X 2, 6. 429, 10. 500). Between the SK and SCC groups (X2, 3.972), between the well-differentiated and low-differentiated groups (X2, 4.020), between the non-lymph node metastasis group and the lymph node metastasis group (X2, 4.788), and between the high-differentiated group and the low-and middle-differentiated group (X2, 4.020). The positive rates of Fhit in SK group, BD group and SCC group were 40.0%, 70.0% and 80.0%, respectively, and the expression of hTERT protein was in control group and SK group, and the expression of Fhit protein was negative in the control group and SK group, and the positive rate was 40.0%, 70.0% and 80.0% in the control group, BD group and SCC group, respectively. There was a significant difference between BD group and SCC group (P < 0 05), between SK group and SCC group (X2, 8. 924), between well-differentiated group and low-and middle-differentiated group (X2, 4.861), and there was no significant difference between the two groups (P < 0. 05). There was also a significant difference between the group without lymph node metastasis and the group with lymph node metastasis (P < 0 05). Between SK group, BD group and SCC group, there was significant difference between the two groups (P < 0 05), and the difference was significant between the two groups (P < 0 05). 3 in SCC group, the higher the positive rate and intensity of FHIT, the lower the positive rate and intensity of hTERT. The results of correlation analysis also showed that the positive expression of FHIT protein was negatively correlated with the positive expression of hTERT protein in SCC group (r = 0.363, p = 0.010). Conclusion: the decreased expression of FHIT protein is related to the carcinogenesis and progression of cutaneous squamous cell carcinoma, and the increased expression of hTERT protein is related to the carcinogenesis and progression of cutaneous squamous cell carcinoma. FHIT and hTERT play an antagonistic role in the development and progression of cutaneous squamous cell carcinoma.
【學(xué)位授予單位】:青島大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2012
【分類號(hào)】:R739.5

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