FHIT、hTERT蛋白在皮膚鱗狀細(xì)胞癌中的表達(dá)及意義
[Abstract]:Objective: to detect the expression of fragile histidine triad (FHIT) and human telomerase reverse transcriptase (hTERT) protein in skin squamous cell carcinoma (SCC), (SCC), Bowen's disease (BD), daylight keratosis (SK) and normal skin tissues. To investigate the relationship between FHIT and hTERT proteins and the genesis and progression of SCC. Methods: 1Wax blocks were collected from 50 cases of SCC,20, 15 cases of SK and 50 cases of BD, and 10 cases of normal skin were sectioned continuously. 2 Immunohistochemistry PV-6000 method was used to detect the expression of FHIT and hTERT protein in the above tissues. To compare the expression of hTERT and FHIT between SCC,SK,BD and control skin. Results: the positive rates of 1FHIT expression in control group, SK group, BD group and SCC group were 100. 0%, 73. 3%, 55. 0% and 44. 0%, respectively. The expression of Fhit protein was between control group and BD group and SCC group (X 2, 6. 429, 10. 500). Between the SK and SCC groups (X2, 3.972), between the well-differentiated and low-differentiated groups (X2, 4.020), between the non-lymph node metastasis group and the lymph node metastasis group (X2, 4.788), and between the high-differentiated group and the low-and middle-differentiated group (X2, 4.020). The positive rates of Fhit in SK group, BD group and SCC group were 40.0%, 70.0% and 80.0%, respectively, and the expression of hTERT protein was in control group and SK group, and the expression of Fhit protein was negative in the control group and SK group, and the positive rate was 40.0%, 70.0% and 80.0% in the control group, BD group and SCC group, respectively. There was a significant difference between BD group and SCC group (P < 0 05), between SK group and SCC group (X2, 8. 924), between well-differentiated group and low-and middle-differentiated group (X2, 4.861), and there was no significant difference between the two groups (P < 0. 05). There was also a significant difference between the group without lymph node metastasis and the group with lymph node metastasis (P < 0 05). Between SK group, BD group and SCC group, there was significant difference between the two groups (P < 0 05), and the difference was significant between the two groups (P < 0 05). 3 in SCC group, the higher the positive rate and intensity of FHIT, the lower the positive rate and intensity of hTERT. The results of correlation analysis also showed that the positive expression of FHIT protein was negatively correlated with the positive expression of hTERT protein in SCC group (r = 0.363, p = 0.010). Conclusion: the decreased expression of FHIT protein is related to the carcinogenesis and progression of cutaneous squamous cell carcinoma, and the increased expression of hTERT protein is related to the carcinogenesis and progression of cutaneous squamous cell carcinoma. FHIT and hTERT play an antagonistic role in the development and progression of cutaneous squamous cell carcinoma.
【學(xué)位授予單位】:青島大學(xué)
【學(xué)位級(jí)別】:碩士
【學(xué)位授予年份】:2012
【分類號(hào)】:R739.5
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