【摘要】：目的：通過檢測(cè)脆性組氨酸三聯(lián)體(FHIT)及人類端粒酶逆轉(zhuǎn)錄酶(hTERT)蛋白在皮膚鱗狀細(xì)胞癌(SCC)、鮑溫病(BD)、日光角化病(SK)及正常對(duì)照皮膚組織中的表達(dá)情況,探討FHIT及hTERT蛋白與SCC發(fā)生及演進(jìn)的關(guān)系。 方法：①收集經(jīng)病理確診的50例SCC、20例BD及15例SK的蠟塊,10例對(duì)照皮膚的蠟塊,均進(jìn)行連續(xù)切片。②采用免疫組化PV-6000法檢測(cè)上述組織中FHIT和hTERT蛋白的表達(dá)。比較SCC、SK、BD及對(duì)照皮膚之間hTERT及FHIT的表達(dá)情況。 結(jié)果：①FHIT在對(duì)照組、SK組、BD組及SCC組的表達(dá)陽性率分別為100.0%、73.3%、55.0%和44.0%,FHIT蛋白的表達(dá)在對(duì)照組和BD組、SCC組之間(X2=6.429、10.500),SK組和SCC組之間(X2=3.972),高分化組和中低分化組之間(X2=4.020),無淋巴結(jié)轉(zhuǎn)移組和有淋巴結(jié)轉(zhuǎn)移組之間(X2=4.788),差異均有顯著性(p均＜0.05)。FHIT在對(duì)照組皮膚均為陰性,在SK組、BD組及SCC組的表達(dá)陽性率分別為40.0%、70.0%和80.0%,hTERT蛋白的表達(dá)在對(duì)照組和SK組、BD組和SCC組之間均有顯著性差異(p均＜0.05),在SK組和SCC組之間(X2=8.924),高分化組和中低分化組之間(X2=4.861),無淋巴結(jié)轉(zhuǎn)移組和有淋巴結(jié)轉(zhuǎn)移組之間(X2=3.947),差異也均有顯著性(p均＜0.05),在SK組與BD組和SCC組之間,差異均無顯著性(p均＞0.05)。③在SCC組中FHIT陽性率、陽性強(qiáng)度越高,其hTERT的表達(dá)陽性率和陽性強(qiáng)度越低。相關(guān)性分析結(jié)果也表明,SCC組中FHIT蛋白的陽性表達(dá)與hTERT蛋白的陽性表達(dá)呈負(fù)相關(guān)(r=-0.363,p=0.010)。 結(jié)論： FHIT蛋白的表達(dá)減少與皮膚鱗狀細(xì)胞癌的發(fā)生及演進(jìn)有關(guān)；hTERT蛋白的表達(dá)增多與皮膚鱗狀細(xì)胞癌的發(fā)生及演進(jìn)有關(guān)；FHIT和hTERT在皮膚鱗狀細(xì)胞癌發(fā)生、演進(jìn)過程中發(fā)揮拮抗作用。
[Abstract]:Objective: to detect the expression of fragile histidine triad (FHIT) and human telomerase reverse transcriptase (hTERT) protein in skin squamous cell carcinoma (SCC), (SCC), Bowen's disease (BD), daylight keratosis (SK) and normal skin tissues. To investigate the relationship between FHIT and hTERT proteins and the genesis and progression of SCC. Methods: 1Wax blocks were collected from 50 cases of SCC,20, 15 cases of SK and 50 cases of BD, and 10 cases of normal skin were sectioned continuously. 2 Immunohistochemistry PV-6000 method was used to detect the expression of FHIT and hTERT protein in the above tissues. To compare the expression of hTERT and FHIT between SCC,SK,BD and control skin. Results: the positive rates of 1FHIT expression in control group, SK group, BD group and SCC group were 100. 0%, 73. 3%, 55. 0% and 44. 0%, respectively. The expression of Fhit protein was between control group and BD group and SCC group (X 2, 6. 429, 10. 500). Between the SK and SCC groups (X2, 3.972), between the well-differentiated and low-differentiated groups (X2, 4.020), between the non-lymph node metastasis group and the lymph node metastasis group (X2, 4.788), and between the high-differentiated group and the low-and middle-differentiated group (X2, 4.020). The positive rates of Fhit in SK group, BD group and SCC group were 40.0%, 70.0% and 80.0%, respectively, and the expression of hTERT protein was in control group and SK group, and the expression of Fhit protein was negative in the control group and SK group, and the positive rate was 40.0%, 70.0% and 80.0% in the control group, BD group and SCC group, respectively. There was a significant difference between BD group and SCC group (P < 0 05), between SK group and SCC group (X2, 8. 924), between well-differentiated group and low-and middle-differentiated group (X2, 4.861), and there was no significant difference between the two groups (P < 0. 05). There was also a significant difference between the group without lymph node metastasis and the group with lymph node metastasis (P < 0 05). Between SK group, BD group and SCC group, there was significant difference between the two groups (P < 0 05), and the difference was significant between the two groups (P < 0 05). 3 in SCC group, the higher the positive rate and intensity of FHIT, the lower the positive rate and intensity of hTERT. The results of correlation analysis also showed that the positive expression of FHIT protein was negatively correlated with the positive expression of hTERT protein in SCC group (r = 0.363, p = 0.010). Conclusion: the decreased expression of FHIT protein is related to the carcinogenesis and progression of cutaneous squamous cell carcinoma, and the increased expression of hTERT protein is related to the carcinogenesis and progression of cutaneous squamous cell carcinoma. FHIT and hTERT play an antagonistic role in the development and progression of cutaneous squamous cell carcinoma.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R739.5

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉艷麗,李學(xué)民,靳國梁,嚴(yán)霞,楊建柱,王俊靈,李月紅,王鳳榮,張祥宏;河北省磁縣食管癌高發(fā)區(qū)食管鱗狀細(xì)胞癌組織HPV檢測(cè)及FHIT表達(dá)的研究[J];癌癥;2003年05期

2 趙坡,宋欣,寧元元,李向紅;肝細(xì)胞癌脆性組氨酸三聯(lián)體Fhit蛋白表達(dá)丟失研究[J];中華肝膽外科雜志;2004年01期

3 鄭虹,吳緒峰,陳惠禎;FHIT蛋白在宮頸癌中的表達(dá)及臨床意義[J];數(shù)理醫(yī)藥學(xué)雜志;2004年03期

4 葛云潔,劉學(xué)東;FHIT基因在肺癌中缺失的研究[J];青島醫(yī)藥衛(wèi)生;2002年02期

5 王莉,董陸佳,田方,劉廣賢,李春海;白血病中FHIT基因的異常表達(dá)和缺失(英文)[J];中國實(shí)驗(yàn)血液學(xué)雜志;2003年02期

6 侯寧,曹友俊,趙坡,李向紅,李維華;前列腺癌脆性組氨酸三體Fhit蛋白高表達(dá)及其意義[J];診斷病理學(xué)雜志;2003年03期

7 朱介之,王波,洪凡真;子宮內(nèi)膜癌組織中FHIT基因啟動(dòng)子區(qū)域CpG島甲基化狀況的研究[J];長(zhǎng)治醫(yī)學(xué)院學(xué)報(bào);2004年04期

8 趙坡,呂亞莉,鐘梅,李志軍;食管癌Fhit蛋白表達(dá)丟失的臨床病理研究[J];腫瘤防治研究;2002年06期

9 田中偉,馮捷,宋向鳳,彭振輝;真核表達(dá)載體pcDNA3-FHIT的構(gòu)建及其在COS-1細(xì)胞中瞬時(shí)表達(dá)(英文)[J];Journal of Nanjing Medical University;2004年05期

10 陳萍,李堅(jiān);FHIT基因與肺癌[J];國外醫(yī)學(xué).呼吸系統(tǒng)分冊(cè);2005年02期

相關(guān)會(huì)議論文 前10條

1 劉林]，

本文編號(hào)：2469122