鉺激光點陣法嫩膚的動物實驗觀察
發(fā)布時間：2019-04-30 06:33
【摘要】：目的：1)觀察Er:YAG點陣激光連續(xù)脈沖照射脫毛后的昆明種小鼠背部皮膚后,皮膚的即刻反應,及顯微鏡下小鼠真皮的厚度、膠原纖維及彈力纖維的變化。2)觀察單位面積內(nèi)脈沖數(shù)固定的情況下,提高激光照射能量后小鼠皮膚即刻反應及顯微鏡下真皮厚度、膠原纖維及彈力纖維的變化。 方法：隨機取108只白色昆明種小鼠,雄性(清潔級)體重20g±3,8-10周齡平均分成三組(高能量照射組,低能量照射組,對照組),每組36只,在單位面積內(nèi)脈沖數(shù)固定的情況下,利用點陣鉺激光以兩種不同能量(100J/cm2,25J/cm2連續(xù)脈沖照射脫毛后的昆明種小鼠背部皮膚,對照組不進行激光照射,按照射后1天,3天,7天,14天,28天,56天取實驗組及對照組小鼠背部皮膚做病理切片測量真皮厚度的變化及特殊染色后觀察膠原纖維和彈力纖維數(shù)量的改變。 結果：1)點陣鉺激光照射昆明種小鼠皮膚后,皮膚即刻出現(xiàn)一系列緊密排列的局灶性點狀汽化剝脫微損傷,能量高的照射組可見局灶性微損傷區(qū)輕度點狀出血；照射后1-2天微損傷區(qū)皮膚發(fā)紅,出現(xiàn)炎癥反應,自3-4天開始炎癥和壞死組織逐漸形成痂皮并脫落,6天內(nèi)痂皮脫落完全；7天-56天顯微鏡下表皮層略有增厚,真皮層增厚明顯,局部膠原纖維新生重塑,照射后14天開始真皮層增厚顯著,且膠原纖維及彈力纖維一直持續(xù)處于增生狀態(tài),至56天時實驗組真皮厚度,膠原纖維及彈力纖維含量仍明顯高于對照組。2)采用不同能量激光照射小鼠背部皮膚后可見高能量實驗組較低能量實驗組真皮厚度增加更為明顯,纖維組織增生較為明顯,但愈合時間也相應略有延長,表明在一定能量范圍內(nèi),隨著點陣激光照射能量的不斷增加,其產(chǎn)生的治療效果也相應增加,但副作用也可能相繼出現(xiàn)。 結論：點陣鉺激光照射昆明種小鼠背部皮膚后可有效的刺激真皮膠原纖維及彈力纖維增生,增加真皮層厚度,從而增強皮膚彈性,達到嫩膚效果,且愈合較為迅速。在一定范圍內(nèi),隨激光照射能量的增加,真皮層厚度及膠原纖維、彈力纖維數(shù)量將增加。
[Abstract]:Objective: 1) to observe the immediate reaction of the back skin of Kunming mice and the thickness of mouse dermis under microscope after Er:YAG lattice laser continuous pulse irradiation on the back skin of Kunming mice after depilation. The changes of collagen fiber and elastic fiber were observed. 2) under the condition of fixed pulse number per unit area, the immediate reaction of skin and the changes of dermal thickness, collagen fiber and elastic fiber under microscope after laser irradiation were increased. Methods: 108 white Kunming mice were randomly divided into three groups (high energy irradiation group, low energy irradiation group, control group), with 36 mice in each group under the condition of constant pulse number per unit area, male (clean grade) weight 20g 鹵3,8 weeks old. Two different energies (100J / cm ~ 2, 25J / cm ~ 2) were used to irradiate the back skin of Kunming mice with two different energies (100J / cm ~ 2, 25J / cm ~ 2). In the control group, the skin of Kunming mice was irradiated at 1, 3, 7, 14, 28 days after irradiation without laser irradiation. The thickness of the dermis and the number of collagen fibers and elastic fibers were measured by pathological sections of the skin of the experimental group and the control group on the 56th day. Results: 1) when Kunming mice skin was irradiated by dot-array erbium laser, a series of focal punctate vaporization and denudation injury occurred immediately, and slight punctate hemorrhage could be seen in the high energy irradiation group. 2 days after irradiation, the skin was red and appeared inflammatory reaction. From 3 to 4 days, the inflammation and necrosis tissue gradually formed and exfoliated, and the skin exfoliated completely within 6 days. From 7 days to 56 days after irradiation, the epidermis was slightly thickened and the epidermis was thickened obviously, and the local collagen fibers were reconstituted. On the 14th day after irradiation, the dermal cortex became thickened significantly, and the collagen fibers and elastic fibers continued to proliferate. At 56 days, the dermal thickness, collagen fiber and elastic fiber contents in the experimental group were still significantly higher than those in the control group. 2) after irradiated with different energy laser, the dermal thickness in the high energy group was more obvious than that in the low energy group. The fibrous tissue hyperplasia was obvious, but the healing time was slightly prolonged, indicating that in a certain energy range, with the increasing energy of lattice laser irradiation, the therapeutic effect also increased, but the side effects might also appear one after another. Conclusion: the lattice erbium laser irradiation on the back of Kunming mice can effectively stimulate the proliferation of dermal collagen fibers and elastic fibers and increase the thickness of dermis so as to enhance the elasticity of the skin and achieve the effect of skin rejuvenation, and the healing is more rapid. In a certain range, with the increase of laser irradiation energy, the thickness of dermis and the number of collagen fibers and elastic fibers will increase.
【學位授予單位】：大連醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R751.05
本文編號：2468560
[Abstract]:Objective: 1) to observe the immediate reaction of the back skin of Kunming mice and the thickness of mouse dermis under microscope after Er:YAG lattice laser continuous pulse irradiation on the back skin of Kunming mice after depilation. The changes of collagen fiber and elastic fiber were observed. 2) under the condition of fixed pulse number per unit area, the immediate reaction of skin and the changes of dermal thickness, collagen fiber and elastic fiber under microscope after laser irradiation were increased. Methods: 108 white Kunming mice were randomly divided into three groups (high energy irradiation group, low energy irradiation group, control group), with 36 mice in each group under the condition of constant pulse number per unit area, male (clean grade) weight 20g 鹵3,8 weeks old. Two different energies (100J / cm ~ 2, 25J / cm ~ 2) were used to irradiate the back skin of Kunming mice with two different energies (100J / cm ~ 2, 25J / cm ~ 2). In the control group, the skin of Kunming mice was irradiated at 1, 3, 7, 14, 28 days after irradiation without laser irradiation. The thickness of the dermis and the number of collagen fibers and elastic fibers were measured by pathological sections of the skin of the experimental group and the control group on the 56th day. Results: 1) when Kunming mice skin was irradiated by dot-array erbium laser, a series of focal punctate vaporization and denudation injury occurred immediately, and slight punctate hemorrhage could be seen in the high energy irradiation group. 2 days after irradiation, the skin was red and appeared inflammatory reaction. From 3 to 4 days, the inflammation and necrosis tissue gradually formed and exfoliated, and the skin exfoliated completely within 6 days. From 7 days to 56 days after irradiation, the epidermis was slightly thickened and the epidermis was thickened obviously, and the local collagen fibers were reconstituted. On the 14th day after irradiation, the dermal cortex became thickened significantly, and the collagen fibers and elastic fibers continued to proliferate. At 56 days, the dermal thickness, collagen fiber and elastic fiber contents in the experimental group were still significantly higher than those in the control group. 2) after irradiated with different energy laser, the dermal thickness in the high energy group was more obvious than that in the low energy group. The fibrous tissue hyperplasia was obvious, but the healing time was slightly prolonged, indicating that in a certain energy range, with the increasing energy of lattice laser irradiation, the therapeutic effect also increased, but the side effects might also appear one after another. Conclusion: the lattice erbium laser irradiation on the back of Kunming mice can effectively stimulate the proliferation of dermal collagen fibers and elastic fibers and increase the thickness of dermis so as to enhance the elasticity of the skin and achieve the effect of skin rejuvenation, and the healing is more rapid. In a certain range, with the increase of laser irradiation energy, the thickness of dermis and the number of collagen fibers and elastic fibers will increase.
【學位授予單位】：大連醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R751.05
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