【摘要】：目的: 1)研究人黑素瘤組織中TrkB的表達情況,探討其在黑素瘤中的作用。2)觀察TrkB在人黑素瘤細胞A875中的表達情況以及在使用雷公藤內(nèi)酯醇作用后A875細胞TrkB表達的變化,探討雷公藤內(nèi)酯醇抗腫瘤的作用機制。 方法: 1)用免疫組化SP法檢測40例人黑素瘤和30例人色素痣組織中TrkB的表達。2)用細胞免疫組化法,RT-PCR法以及western-blot法分別檢測雷公藤內(nèi)酯醇作用前后A875細胞中TrkB的表達情況。 結(jié)果: 1)黑素瘤組織和人色素痣組織中TrkB陽性率分別為77.5%和16.7%,兩者間差異有統(tǒng)計學意義(P0.01)。 2)a〉在細胞免疫組化法中,TrkB在A875細胞中呈陽性表達,雷公藤內(nèi)酯醇作用后其表達下降,兩者間差異有統(tǒng)計學意義(P0.05)。b〉western-blot法顯示雷公藤內(nèi)酯醇處理A875細胞后TrkB蛋白表達明顯低于未處理細胞。c〉RT-PCR法中雷公藤內(nèi)酯醇作用后TrkB的表達明顯低于未使用雷公藤內(nèi)酯醇作用的A875細胞。 結(jié)論: TrkB在人黑素瘤組織中以及人黑素瘤細胞A875中均呈陽性表達,其高表達可能是黑素瘤發(fā)生發(fā)展的原因之一,在對A875細胞的研究中表明雷公藤內(nèi)酯醇能抑制TrkB的表達,提示雷公藤內(nèi)酯醇可能通過抑制TrkB的表達而產(chǎn)生抗腫瘤作用。
[Abstract]:Objective: 1) to study the expression of TrkB in human melanoma. To investigate the role of TrkB in melanoma. 2) to observe the expression of TrkB in human melanoma cell line A875 and the change of TrkB expression in A875 cells treated with triptolide, and to explore the anti-tumor mechanism of triptolide. Methods: 1) immunohistochemical SP method was used to detect the expression of TrkB in 40 cases of human melanoma and 30 cases of human pigmented nevus. 2) the expression of TrkB in 40 cases of human melanoma and 30 cases of human pigmented nevus was detected by immunocytochemistry. RT-PCR and western-blot methods were used to detect the expression of TrkB in A875 cells before and after triptolide treatment. Results: 1) the positive rate of TrkB in melanoma and human pigmented nevus was 77.5% and 16.7%, respectively. There was significant difference between them (P0.01). 2) a > in the cellular immunohistochemical method, the expression of TrkB was positive in A875 cells, and decreased after triptolide treatment. The expression of TrkB protein in A875 cells treated with triptolide was significantly lower than that in untreated cells (P0.05). The expression of TrkB protein in A875 cells treated with triptolide was significantly lower than that in untreated cells (P0.05). The expression of TrkB protein in TrkB treated with triptolide in RT-PCR method was significantly lower than that in untreated cells. The expression was significantly lower than that of A 875 cells treated with triptolide without Tripterygium wilfordii. Conclusion: TrkB is positive in human melanoma tissue and in human melanoma cell line A875, and its high expression may be one of the reasons for the occurrence and development of melanoma. In the study of A875 cell line, triptolide can inhibit the expression of TrkB, and it is suggested that tripterygiolide can inhibit the expression of TNF in human melanoma tissue and human melanoma cell line A875. These results suggest that triptolide may have anti-tumor effect by inhibiting the expression of TrkB.
【學位授予單位】：華中科技大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R739.5

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