皮膚生理指標(biāo)檢測優(yōu)選特應(yīng)性皮炎潤膚劑的實驗研究
發(fā)布時間：2019-02-12 17:32
【摘要】：目的探討以神經(jīng)酰胺、透明質(zhì)酸鈉、丹皮酚、山茶籽油為主要成分研制潤膚劑用于治療特應(yīng)性皮炎(AD)動物模型,并證實其有效性和安全性。方法按L9(34)正交實驗表設(shè)計4種主要成分的配比,采用四因素三水平安排實驗,將4~6周BALB/c小鼠制備的AD動物模型分為9組,分別涂抹9種主要成分濃度不一的潤膚劑,通過檢測治療前及治療后的皮膚含水量、經(jīng)皮水分丟失(TEWL)篩選最優(yōu)處方,并進(jìn)行藥效學(xué)及皮膚刺激性實驗。結(jié)果通過正交表的極差分析以及方差分析得出神經(jīng)酰胺對皮膚含水量影響最大,山茶籽油對TEWL影響最大,最優(yōu)的處方為1號,主要成分的配比為D1E1F1G1;藥效學(xué)實驗顯示高、中、低劑量組與空白對照組相比治療后的皮膚含水量、pH值、TEWL值均明顯改善,且P值均0.05,與陽性對照組相比無統(tǒng)計學(xué)差異,P值均0.05;皮膚刺激性試驗結(jié)果為0分,無刺激性。結(jié)論該潤膚劑能明顯改善AD小鼠皮膚含水量、pH、TEWL,可用于修復(fù)AD小鼠的皮膚屏障,且無刺激性。
[Abstract]:Objective to study the efficacy and safety of ceramide, sodium hyaluronate, paeonol and camellia seed oil in the treatment of atopic dermatitis (AD). Methods according to the L9 (34) orthogonal experiment table, four factors and three levels were used to arrange the experiment. The AD animal model was divided into 9 groups, and 9 kinds of emollients with different concentrations were applied respectively. The optimal prescription was screened by detecting the skin moisture content before and after treatment and skin moisture loss by (TEWL). The pharmacodynamics and skin irritation experiments were also carried out. Results through the range analysis and variance analysis of orthogonal table, it was found that ceramide had the greatest effect on skin water content, and Camellia camellia seed oil had the biggest effect on TEWL, the best prescription was 1, and the proportion of main components was D1E1F1G1. The pharmacodynamics test showed that the skin water content, pH value and TEWL value of the high, middle and low dose groups were significantly improved compared with the blank control group, and the P value was 0.05, and there was no statistical difference between the two groups (P value 0.05). The results of skin irritation test were 0, no irritation. Conclusion the skin moisture content of AD mice can be significantly improved by this moisturizer, and pH,TEWL, can be used to repair the skin barrier of AD mice without irritation.
【作者單位】： 南方醫(yī)科大學(xué)第三附屬醫(yī)院皮膚科;南方醫(yī)科大學(xué);南方醫(yī)科大學(xué)深圳醫(yī)院藥劑科;南方醫(yī)科大學(xué)南方醫(yī)院藥學(xué)部;
【基金】：天河區(qū)科技計劃項目(201404KW048)
【分類號】：R758.2
[Abstract]:Objective to study the efficacy and safety of ceramide, sodium hyaluronate, paeonol and camellia seed oil in the treatment of atopic dermatitis (AD). Methods according to the L9 (34) orthogonal experiment table, four factors and three levels were used to arrange the experiment. The AD animal model was divided into 9 groups, and 9 kinds of emollients with different concentrations were applied respectively. The optimal prescription was screened by detecting the skin moisture content before and after treatment and skin moisture loss by (TEWL). The pharmacodynamics and skin irritation experiments were also carried out. Results through the range analysis and variance analysis of orthogonal table, it was found that ceramide had the greatest effect on skin water content, and Camellia camellia seed oil had the biggest effect on TEWL, the best prescription was 1, and the proportion of main components was D1E1F1G1. The pharmacodynamics test showed that the skin water content, pH value and TEWL value of the high, middle and low dose groups were significantly improved compared with the blank control group, and the P value was 0.05, and there was no statistical difference between the two groups (P value 0.05). The results of skin irritation test were 0, no irritation. Conclusion the skin moisture content of AD mice can be significantly improved by this moisturizer, and pH,TEWL, can be used to repair the skin barrier of AD mice without irritation.
【作者單位】： 南方醫(yī)科大學(xué)第三附屬醫(yī)院皮膚科;南方醫(yī)科大學(xué);南方醫(yī)科大學(xué)深圳醫(yī)院藥劑科;南方醫(yī)科大學(xué)南方醫(yī)院藥學(xué)部;
【基金】：天河區(qū)科技計劃項目(201404KW048)
【分類號】：R758.2
【參考文獻(xiàn)】
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