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miR-203對HaCaT細胞p63和SOCS3表達調(diào)控初步研究

發(fā)布時間:2019-02-11 09:26
【摘要】:目的:銀屑病(psoriasis)是嚴重影響人類身心健康的自身免疫性皮膚病。導致銀屑病表皮細胞過度增殖和異常分化的確切原因尚未確定。miRNAs是內(nèi)源性非蛋白編碼單鏈小分子RNAs,主要功能為轉(zhuǎn)錄后水平抑制靶基因的表達。miRNAs對其靶基因的調(diào)節(jié)障礙可導致不同疾病的發(fā)生。miR-203具有高度皮膚特異性,在銀屑病皮損中異常表達,,有報道推測p63和SOCS3是其進化保守的靶基因。miR-203對p63和SOCS3的調(diào)節(jié)及其在角質(zhì)形成細胞增殖、分化和銀屑病發(fā)病機制中的作用尚不明確。STAT3和Notch信號通路在銀屑病的發(fā)病中起著關鍵的作用,而p63和SOCS3是上述信號通路的2個重要調(diào)節(jié)子。 本研究旨在通過將miR-203的模擬物和其抑制物轉(zhuǎn)染人永生化角質(zhì)形成細胞HaCaT細胞系,確定其對p63和SOCS3的調(diào)控機制、明確其與STAT3和Notch信號通路的關系及其在銀屑病發(fā)病機制中的作用,確定它們的調(diào)控方式和相互關系,為其作為銀屑病新的治療靶標提供理論依據(jù)。 方法:用miR-203的模擬物和抑制物(miR-203mimic和miR-203inhibitor)定量轉(zhuǎn)染人角質(zhì)形成細胞系HaCaT細胞。通過qRT-PCR、Western blot、流式細胞術方法檢測p63、SOCS3、p-STAT3和Notch1基因及蛋白的表達水平;并用含TNF-α (20ng/ml)和IL-6(20ng/ml)的培養(yǎng)液處理HaCaT細胞,并以qRT-PCR法對miR-203的表達水平進行檢測,觀察細胞因子TNF-α和IL-6對miR-203表達水平的影響。 結果:1. qRT-PCR檢測p63、SOCS3、p-STAT3、Notch1的mRNA表達水平,其中轉(zhuǎn)染miR-203mimic組與對照組相比SOCS3、Notch1、p-STAT3的mRNA表達上調(diào),Notch1組間差異具有顯著性(P 0.05),p63mRNA表達水平無明顯變化;轉(zhuǎn)染miR-203inhibitor組與對照組相比SOCS3、Notch1、p-STAT3的mRNA表達下調(diào),SOCS3和Notch1組間差異有顯著性(P 0.05),p63mRNA表達亦無明顯變化。2. Western blot和流式細胞術方法檢測蛋白表達,轉(zhuǎn)染miR-203mimic的細胞,p63和SOCS3表達下降,p-STAT3和Notch1表達升高;轉(zhuǎn)染miR-203inhibitor的細胞結果與之相反。3. HaCaT細胞經(jīng)TNF-α和IL-6處理后,經(jīng)qRT-PCR方法檢測miR-203表達升高,差異具有顯著性(P 0.05)。 結論:miR-203的過表達,可以抑制p63和SOCS3表達,從而使STAT3持續(xù)激活,STAT3的磷酸化水平增加,導致角質(zhì)形成細胞過度增殖。本研究為探討miR-203對p63、SOCS3的調(diào)控機制及其與STAT3信號轉(zhuǎn)導通路的關系提供了初步的實驗基礎,有助于了解miR-203在銀屑病發(fā)病機制中的作用。
[Abstract]:Objective: psoriatic (psoriasis) is an autoimmune dermatosis that seriously affects human physical and mental health. The exact cause of excessive proliferation and abnormal differentiation of psoriatic epidermal cells has not been determined. MiRNAs is an endogenous non-protein encoded single-stranded small molecule RNAs,. The main function of miR-203 is to inhibit the expression of target gene at post-transcriptional level. The regulation of target gene by miRNAs may lead to the occurrence of different diseases. MiR-203 has high skin specificity and abnormal expression in psoriatic lesions. It has been reported that p63 and SOCS3 are the conserved target genes of p63 and SOCS3. MiR-203 regulates p63 and SOCS3 and proliferates in keratinocytes. The role of differentiation and psoriasis pathogenesis is unclear. STAT3 and Notch signaling pathway play a key role in psoriasis pathogenesis, while p63 and SOCS3 are two important regulators of these signaling pathways. The aim of this study was to determine the regulatory mechanism of miR-203 on p63 and SOCS3 by transfection of immortalized human keratinocytes HaCaT cell line with miR-203 mimic and its inhibitor. To clarify its relationship with STAT3 and Notch signaling pathway and its role in the pathogenesis of psoriasis, to determine their regulatory mode and mutual relationship, to provide a theoretical basis for its new therapeutic target for psoriasis. Methods: human keratinocytes HaCaT cells were quantitatively transfected with miR-203 mimics and inhibitors (miR-203mimic and miR-203inhibitor). The expression of p-STAT3 and Notch1 genes and proteins were detected by qRT-PCR,Western blot, flow cytometry. HaCaT cells were treated with TNF- 偽 (20ng/ml) and IL-6 (20ng/ml), and the expression of miR-203 was detected by qRT-PCR method. The effects of cytokines TNF- 偽 and IL-6 on miR-203 expression were observed. Results: 1. QRT-PCR was used to detect the mRNA expression of p63- SOCS3pSTAT3pSTAT3ntch1. The expression of SOCS3,Notch1,p-STAT3 mRNA was up-regulated in the transfected miR-203mimic group compared with the control group. There was significant difference between the Notch1 group and the Notch1 group (P 0.05), but the p63mRNA expression level did not change significantly. Compared with the control group, the mRNA expression of SOCS3,Notch1,p-STAT3 in miR-203inhibitor transfected group was down-regulated, the difference between SOCS3 and Notch1 group was significant (P 0.05), and the expression of p63mRNA was not changed significantly. 2. Western blot and flow cytometry were used to detect the protein expression. The expression of p63 and SOCS3 decreased and the expression of p-STAT3 and Notch1 increased in the cells transfected with miR-203mimic, whereas the expression of p-STAT3 and Notch1 was increased in the transfected miR-203inhibitor cells. After HaCaT cells were treated with TNF- 偽 and IL-6, the expression of miR-203 was detected by qRT-PCR method, and the difference was significant (P 0.05). Conclusion: overexpression of miR-203 can inhibit the expression of p63 and SOCS3, which leads to the sustained activation of STAT3 and the increase of phosphorylation of STAT3, resulting in excessive proliferation of keratinocytes. This study provides a preliminary experimental basis for the study of the regulatory mechanism of miR-203 on p63 SOCS3 and its relationship with STAT3 signal transduction pathway, and helps to understand the role of miR-203 in the pathogenesis of psoriasis.
【學位授予單位】:大連醫(yī)科大學
【學位級別】:碩士
【學位授予年份】:2013
【分類號】:R758.63

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