【摘要】： 目的：檢測同源結(jié)構域相互作用蛋白激酶2(HIPK2)和細胞周期蛋白D1(cyclin D1)在鮑溫病和鱗狀細胞癌中的表達,以探討它們在腫瘤發(fā)生發(fā)展中的作用與關系。 方法：鮑溫病(BD)和鱗狀細胞癌(SCC)的皮損標本來源于2007年至2010年復旦大學附屬華山醫(yī)院皮膚科門診病人,共計54例,并經(jīng)組織病理確診。其中24例為皮膚鱗狀細胞癌,30例為鮑溫病,8例為正常對照。取患者皮損組織用于常規(guī)病理分析并采用免疫組化學方法檢測鮑溫病組織、鱗狀細胞癌組織、正常皮膚組織中HIPK2和cyclin D1的表達水平。 結(jié)果：在鮑溫病組中HIPK2蛋白陽性率為90%(27／30),在鱗狀細胞癌組中HIPK2的陽性率為91.7%(22／24),在對照組HIPK2蛋白陽性率為100%(8／8)。HIPK2的表達在鱗狀細胞癌組及鮑溫病組中與正常對照組相比,差異無顯著性(p0.05)；鮑溫病組與鱗狀細胞癌組間差異無顯著性意義(p0.05)。但從病理角度來看,HIPK2在表皮基底細胞增殖層細胞核內(nèi)表達,而在表皮非增殖層它的表達逐漸消失。HIPK2在鮑溫病中表達主要位于非典型增生的區(qū)域的細胞核內(nèi)而在鱗狀細胞癌中表達在癌巢邊細胞和癌巢中央細胞。Cyclin D1的陽性率在鮑溫病組中為43.3%(13／30),在鱗狀細胞癌組中cyclin D1的陽性率為70.8%(17／24),在對照組cyclin D1陽性率為0%(0／8)。在鱗狀細胞癌組及鮑溫病組中cyclin D1的表達比正常對照組明顯增高,差異有統(tǒng)計學意義,且鮑溫病組與鱗狀細胞癌組間差異有統(tǒng)計學意義(p0.05)。HIPK2和cyclin D1的表達與鱗狀細胞癌的病理分化程度差異無統(tǒng)計學意義(p0.05)。在鮑溫病組及狀細胞癌組中cyclin D1與HIPK2兩者分布無相關性。 結(jié)論：HIPK2的表達在皮膚腫瘤中可能起著細胞增殖作用而不是生長抑制功能。Cyclin D1的表達在皮膚細胞的癌變過程中為一早期分子,對皮膚腫瘤發(fā)生、發(fā)展有重要作用,而且cyclin D1在鱗狀細胞癌中的表達不受分化程度的影響。
[Abstract]:Aim: to investigate the expression of homologous domain interacting protein kinase 2 (HIPK2) and cyclin D1 (cyclin D1) in Baldwin's disease and squamous cell carcinoma (SCC). Methods: the lesions of (BD) and (SCC) from 2007 to 2010 were collected from 54 outpatients in the Department of Dermatology affiliated to Fudan University and confirmed by histopathology. There were 24 cases of cutaneous squamous cell carcinoma, 30 cases of Bowen's disease and 8 cases of normal control. The expressions of HIPK2 and cyclin D1 were detected by immunohistochemical method in the tissues of Balwin's disease, squamous cell carcinoma and normal skin. Results: the positive rate of HIPK2 protein was 90% (27 / 30) in Bowen's disease group and 91.7% (22 / 24) in squamous cell carcinoma group. The positive rate of HIPK2 protein in the control group was 100% (8 / 8). There was no significant difference in the expression of HIPK2 between the squamous cell carcinoma group and the Bowen disease group compared with the normal control group (p0. 05). There was no significant difference between Bowen disease group and squamous cell carcinoma group (p 0.05). However, from the pathological point of view, HIPK2 was expressed in the nucleus of epidermal basal cell proliferating layer. In the epidermal non-proliferative layer, the expression of HIPK2 was mainly located in the nucleus of atypical proliferative region and in squamous cell carcinoma. The positive expression of Cyclin D1 was found in the nesting cell and the central cell of the cancer nest. The sex rate in the Bowen group was 43.3% (13 / 30). The positive rate of cyclin D1 was 70.8% (17 / 24) in squamous cell carcinoma group and 0% (0 / 8) in control group. The expression of cyclin D1 in squamous cell carcinoma group and Bowen disease group was significantly higher than that in normal control group, and the difference was statistically significant. The expression of HIPK2 and cyclin D1 was not significantly different from that of squamous cell carcinoma (p0.05). There was no correlation between cyclin D1 and HIPK2 in patients with Bowen's disease and in patients with HCC. Conclusion: the expression of HIPK2 may play a role in cell proliferation rather than growth inhibition in skin tumors. The expression of Cyclin D1 may be an early molecule in the carcinogenesis of skin cells, and may play an important role in the occurrence and development of skin tumors. Moreover, the expression of cyclin D1 in squamous cell carcinoma is not affected by the degree of differentiation.
【學位授予單位】：復旦大學
【學位級別】：碩士
【學位授予年份】：2010
【分類號】：R739.5

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