發(fā)布時間：2019-01-07 19:08

【摘要】：目的：(1)檢測β-防御素2 (HBD-2)、TNF-α、NF-кB p65在尋常型銀屑病皮損中的表達,研究HBD-2、TNF-α、NF-кB p65的表達與銀屑病嚴重程度的關系；(2)探討三者在銀屑病發(fā)生發(fā)展中的作用。 方法：(1)采用免疫組化SP法(鏈霉菌抗生物素蛋白-過氧化物酶連接法)檢測45例銀屑病患者皮損組織、45例銀屑病患者非皮損組織及15例正常皮膚組織標本中HBD-2、TNF-α、NF-кB p65的表達,比較不同組別間三者表達的差異,并對HBD-2、TNF-α、NF-кB p65進行相關性分析。(2)根據PASI評分將45例銀屑病患者分為輕、中、重三組(輕度25例、中度12例、重度8例),檢測輕、中、重銀屑病患者HBD-2、TNF-α、NF-кB p65的表達,比較不同組別間三者表達的差異,分析三者在銀屑病皮損中的相關性。 結果：(1)與正常人皮膚組織和銀屑病患者非皮損區(qū)相比,銀屑病患者皮損區(qū)HBD-2、TNF-α、NF-кB p65的表達顯著增強,非皮損區(qū)HBD-2、TNF-α的表達也高于正常人皮膚組織,差異均有統(tǒng)計學意義(P0.05),非皮損區(qū)NF-кB p65的表達與正常人皮膚組織相比差異無統(tǒng)計學意義(P0.05)。在正常皮膚組織、尋常型銀屑病非皮損區(qū)和銀屑病皮損區(qū)之間HBD-2與TNF-α, TNF-與NF-кB p65, HBD-2與NF-кB p65均存在正相關(P0.05)。(2)在銀屑病皮損區(qū),對HBD-2、TNF-α, NF-кB p65在輕度組、中度組、重度組的表達進行比較,重度組銀屑病患者三者的表達高于中度組,中度組又高于輕度組,并具有統(tǒng)計學意義(P0.05)。 結論：1、HBD-2, TNF-α在正常皮膚組織、尋常型銀屑病非皮損區(qū)、尋常型銀屑病皮損區(qū)中的表達逐漸增強,NF-кB p65在尋常型銀屑病皮損區(qū)顯著高表達。2、HBD-2, TNF-α, NF-кB p65在尋常型銀屑病皮損中的表達均升高,且皮損愈重,表達愈強；HBD-2與TNF-α, TNF-α與NF-кB p65, HBD-2與NF-кB p65表達水平之間均存在正相關。 綜上所述,HBD-2, TNF-α, NF-кB p65與銀屑病的發(fā)生、發(fā)展密切相關,且三者呈協(xié)同作用。三者相互促進、相互增強,加重了銀屑病皮損。
[Abstract]:Objective: (1) to detect the expression of 尾 -defensin 2 (HBD-2), TNF- 偽 and NF- B p65 in psoriatic lesions, and to study the relationship between the expression of HBD-2,TNF- 偽, NF- B p65 and the severity of psoriasis. (2) to explore the role of three factors in the occurrence and development of psoriasis. Methods: (1) Immunohistochemical SP method was used to detect HBD-2, in 45 cases of psoriasis, 45 cases of psoriasis and 15 cases of normal skin tissue. The expression of TNF- 偽 and NF- B p65 were compared among different groups, and the correlation between HBD-2,TNF- 偽 and NF- B p65 was analyzed. (2) according to PASI score, 45 patients with psoriasis were divided into mild and moderate psoriatic patients. The expression of HBD-2,TNF- 偽 and NF- B p65 in patients with mild, moderate and severe psoriasis were detected in three groups (mild 25 cases, moderate 12 cases, severe 8 cases). To analyze the correlation among the three factors in psoriatic lesions. Results: (1) the expression of HBD-2,TNF- 偽 and NF- B p65 in psoriatic lesions was significantly higher than that in normal skin tissues and psoriatic lesions, and the expression of HBD-2, in non-lesion areas was significantly higher than that in normal skin tissues and psoriatic lesions. The expression of TNF- 偽 was significantly higher than that of normal skin tissue (P0.05), while the expression of NF- B p65 in non-lesion area was not significantly different from that in normal skin tissue (P0.05). In normal skin tissue, there was a positive correlation between HBD-2 and TNF- 偽, TNF- and NF- B p65, HBD-2 and NF- B p65 between psoriasis vulgaris and psoriasis lesions (P0.05). (2). The expression of HBD-2,TNF- 偽 and NF- B p65 in mild group, moderate group and severe group were compared. The expression of HBD-2,TNF- 偽 in severe group was higher than that in moderate group, and the expression in moderate group was higher than that in mild group (P0.05). Conclusion: 1 the expression of HBD-2 and TNF- 偽 in normal skin, psoriasis vulgaris, psoriasis vulgaris and psoriasis vulgaris were gradually increased, and the expression of NF- p65 in psoriasis vulgaris was significantly higher than that in psoriasis vulgaris, and the expression of HBD-2in psoriasis vulgaris was significantly higher than that in psoriasis vulgaris. The expressions of TNF- 偽 and NF- p65 in psoriasis vulgaris were all increased, and the more severe the lesions, the stronger the expression. There was a positive correlation between the expression levels of HBD-2 and TNF- 偽, TNF- 偽 and NF- B p65, HBD-2 and NF- B p65. In conclusion, HBD-2, TNF- 偽, NF- B p65 are closely related to the occurrence and development of psoriasis, and they have synergistic effects. The three promote each other, reinforce each other and aggravate psoriatic lesions.
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2011
【分類號】：R758.63

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