【摘要】：目的：觀察基質(zhì)金屬蛋白酶抑制因子(RECK)和基質(zhì)金屬蛋白酶-2(MMP-2)在皮膚鱗狀細(xì)胞癌(CSCC)、鮑溫氏病、非典型增生組織中的表達(dá),并探討其與腫瘤發(fā)生、浸潤(rùn)轉(zhuǎn)移的關(guān)系。 方法：采用免疫組化PV6000法檢測(cè)55例(?)JCSCC組織(其中高分化27例、中分化18例、低分化10例；伴淋巴轉(zhuǎn)移10例,遠(yuǎn)處轉(zhuǎn)移2例)、13例鮑溫氏病、24例不典型增生組織以及20例正常皮膚組織中的RECK和MMP-2蛋白表達(dá),進(jìn)而比較鱗狀細(xì)胞癌在不同分化組、轉(zhuǎn)移組、年齡及性別組中的表達(dá)情況,并將之結(jié)果進(jìn)行統(tǒng)計(jì)學(xué)分析。 結(jié)果： 1鏡下觀察RECK的陽性表達(dá)主要位于正常鱗狀上皮細(xì)胞及腫瘤細(xì)胞的細(xì)胞質(zhì)中,呈淺黃色至棕黃色顆粒,部分胞核染色。MMP-2的陽性染色多定位于癌組織細(xì)胞質(zhì),呈淺黃色至棕黃色顆粒。 2 RECK在正常皮膚組織(CE)、非典型增生(AH)、鮑溫氏病(BD)、皮膚鱗狀細(xì)胞癌(CSCC)四種組織中的表達(dá)RECK在正常皮膚組織、非典型增生、鮑溫氏病、皮膚鱗狀細(xì)胞癌中的表達(dá)率分別為85.0%、54.2%、46.2%、43.6%,比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 3 MMP-2蛋白正常皮膚組織(CE)、非典型增生(AH)、鮑溫氏病(BD)、皮膚鱗狀細(xì)胞癌(CSCC)四種組織中的表達(dá)MMP-2蛋白在正常皮膚組織、非典型增生、鮑溫氏病、皮膚鱗狀細(xì)胞癌中的表達(dá)率分別為25.0%、50.0%、53.8%、63.6%,比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 4 RECK及MMP-2蛋白表達(dá)與皮膚鱗狀細(xì)胞癌(CSCC)的組織學(xué)分級(jí)的關(guān)系根據(jù)組織學(xué)分級(jí),皮膚鱗狀細(xì)胞癌組織分為高中低分化三組。RECK陽性表達(dá)率隨著腫瘤分化程度的降低而降低；而MMP-2陽性表達(dá)率隨著腫瘤分化程度的降低而增高。差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。 5 RECK及MMP-2蛋白與皮膚鱗狀細(xì)胞癌(CSCC)淋巴轉(zhuǎn)移和遠(yuǎn)處轉(zhuǎn)移的關(guān)系無淋巴轉(zhuǎn)移組中RECK陽性表達(dá)率明顯高于淋巴轉(zhuǎn)移組,比較差異有統(tǒng)計(jì)學(xué)意義(P0.01)。MMP-2在有淋巴轉(zhuǎn)移的CSCC組中陽性表達(dá)率明顯高于無淋巴轉(zhuǎn)移組,兩者表達(dá)差異有統(tǒng)計(jì)學(xué)意義(P0.01)。RECK (?)口MMP-2與鱗狀細(xì)胞癌是否存在遠(yuǎn)處轉(zhuǎn)移無關(guān),表達(dá)無統(tǒng)計(jì)學(xué)意義(P0.05)。 6 RECK及MMP-2蛋白與皮膚鱗狀細(xì)胞癌(CSCC)年齡和性別的關(guān)系RECK、MMP-2蛋白表達(dá)與皮膚鱗狀細(xì)胞癌患者的年齡及性別無關(guān),差異無統(tǒng)計(jì)學(xué)意義(P0.05)。 7 RECK與MMP-2在皮膚鱗狀細(xì)胞癌(CSCC)中表達(dá)的關(guān)系RECK及MMP-2在皮膚鱗狀細(xì)胞癌中的表達(dá)呈負(fù)相關(guān)。(r=-0.402 P0.01)。 結(jié)論： 1研究顯示RECK在正常皮膚、非典型增生、鮑溫氏病及鱗狀細(xì)胞癌中均有表達(dá),但表達(dá)水平依次減少,而MMP-2的表達(dá)水平依次增高,提示RECK的表達(dá)下調(diào)或缺失及MMP-2的過度表達(dá)在鱗狀細(xì)胞癌的發(fā)生發(fā)展過程中可能起著重要作用。 2隨著腫瘤組織學(xué)分化程度降低,RECK (?)日性率顯著降低,MMP-2表達(dá)升高,提示RECK作為抑癌因子可能通過誘導(dǎo)細(xì)胞分化從而抑制鱗狀細(xì)胞癌的發(fā)生,而MMP-2的上調(diào)表達(dá)則抑制了細(xì)胞分化過程,從而導(dǎo)致腫瘤惡性程度增高。兩者可能為判斷惡性程度提供依據(jù)。 3本研究發(fā)現(xiàn)RECK、MMP-2蛋白表達(dá)與淋巴結(jié)轉(zhuǎn)移的發(fā)生顯著相關(guān),提示RECK表達(dá)降低,而MMP-2蛋白表達(dá)上調(diào)的腫瘤具有更強(qiáng)的侵蝕和轉(zhuǎn)移能力。 4在鱗狀細(xì)胞癌組織中RECK的低表達(dá)與MMP-2的表達(dá)升高有關(guān)。在皮膚鱗狀細(xì)胞癌的發(fā)生發(fā)展過程中,兩者呈顯著的負(fù)相關(guān)。提示鱗狀細(xì)胞癌組織中RECK表達(dá)的缺失導(dǎo)致了對(duì)MMP-2的抑制作用降低,而使MMP-2表達(dá)上調(diào),進(jìn)而促進(jìn)了腫瘤細(xì)胞的侵襲和轉(zhuǎn)移。
[Abstract]:Objective: To observe the expression of matrix metalloproteinase-2 (REK) and matrix metalloproteinase-2 (MMP-2) in skin squamous cell carcinoma (CSCC), Abalone's disease and atypical hyperplasia. Methods: 55 cases were detected by immunohistochemical PV6000 method. (?) JCSCC (in which 27 cases of high differentiation, 18 cases of medium differentiation, 10 with low differentiation, 10 cases with lymph node metastasis, 2 cases of distant metastasis), 13 cases of Bowen's disease, 24 cases of atypical hyperplasia and 20 normal skin tissues. The expression of squamous cell carcinoma in different group, transfer group, age and sex group was compared, and the results were statistically analyzed. Analysis. Results: The positive expression of RECK was mainly in the cytoplasm of normal squamous cell and tumor cells, and was pale yellow to brown yellow particles. The positive staining of MMP-2 was localized in the cytoplasm of the carcinoma and was pale yellow to the cytoplasm. The expression rate of REK in four tissues of normal skin tissue (CE), atypical hyperplasia (AH), Abalone's disease (BD) and skin squamous cell carcinoma (CSCC) was 85.0, respectively.%, 54.2%, 46.2%, 43.6%, and the difference was of statistical significance. (P0.05) The expression of MMP-2 in four tissues of normal skin tissue (CE), atypical hyperplasia (AH), Bowen's disease (BD), and skin squamous cell carcinoma (CSCC) in the normal skin tissue, atypical hyperplasia, Bowen's disease and squamous cell carcinoma of the skin. The rates were 25. 0%, 50. 0%, 53. 8%, 63.6%, respectively. Significance (P0.05). The relationship between the expression of RECK and MMP-2 and the histological grade of skin squamous cell carcinoma (CSCC) is based on the histological grade, squamous cell carcinoma of the skin. The positive rate of the expression of MMP-2 decreased with the decrease of the degree of differentiation of the tumor, while the positive rate of MMP-2 was with the swelling. The difference in the degree of differentiation of the tumor is increased. The relationship between the lymph node metastasis and the distant metastasis of the squamous cell carcinoma (CSCC) of the skin squamous cell carcinoma (CSCC) and the relationship between the lymph node metastasis and the distant metastasis of the skin squamous cell carcinoma (CSCC) The positive rate of expression of MMP-2 in the group of CSCC with lymphatic metastasis was significantly higher than that of the non-lymphoid metastasis group. (P 0.01). The expression of MMP-2 in the RECK (?) port was not related to the metastasis of the squamous cell carcinoma, and the expression of MMP-2 was not related to the metastasis of the squamous cell carcinoma. There was no statistical significance (P0.05). The relationship between the age and sex of the skin squamous cell carcinoma (CSCC) was not related to the age and the sex of the skin squamous cell carcinoma (CSCC), and the expression of MMP-2 was not related to the age and sex of the skin squamous cell carcinoma. The relationship between the expression of MMP-2 in the skin squamous cell carcinoma (CSCC) and the expression of MMP-2 in the skin squamous cell carcinoma (CSCC) were not significant (P0.05). The expression of 2 in squamous cell carcinoma of the skin is negative. Off. (r =-0.402 p0.01). Conclusion: 1 The expression of RECK in normal skin, atypical hyperplasia, Bowen's disease and squamous cell carcinoma is shown, but water is expressed. The level of expression of MMP-2 was decreased, and the expression level of MMP-2 was increased in this order, suggesting that the expression of RECK was down-regulated or deleted and the expression of MMP-2 was overexpressed. The development of squamous cell carcinoma may play an important role in the development of squamous cell carcinoma.. 2. With the decrease of the degree of histological differentiation of the tumor, the rate of REK (?) was significantly lower, and the expression of MMP-2 increased, and the expression of RECK as a cancer-inhibiting factor could inhibit the occurrence of squamous cell carcinoma by inducing the differentiation of the cells, and the up-regulation of MMP-2 then the cell differentiation process is suppressed, resulting in a swelling In this study, the expression of RECK and MMP-2 was associated with the occurrence of lymph node metastasis, and the expression of REK was decreased, and M MP-2 protein expression up-regulated tumor has stronger erosion and transfer ability. 4 in scale The low expression of RECK in the cell carcinoma is related to the increase of the expression of MMP-2. In the course of the development of squamous cell carcinoma of the skin, there was a significant negative correlation between the two groups. The deletion of the expression of the REK in the squamous cell carcinoma of the skin resulted in a decrease in the inhibition of MMP-2.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R739.5

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本文編號(hào)：2402133