【摘要】：目的研究Toll樣受體(Toll-like receptor,TLR)2.4mRNA在麻風(fēng)患者外周血單個(gè)核細(xì)胞中的表達(dá)及其相關(guān)位點(diǎn)(TLR2-597T/C.TLR4-896A/G)基因單核苷酸多態(tài)性(single nucleotide polymorphisms, SNP),探討其與麻風(fēng)患者免疫的聯(lián)系以及在麻風(fēng)感染中的作用機(jī)制。方法選擇中國貴州畢節(jié)地區(qū)麻風(fēng)病例150例,其中瘤型103例,結(jié)核樣型47例,家族集聚性者43例,非家族集聚性者107例,用RT-PCR法檢測麻風(fēng)患者外周血單個(gè)核細(xì)胞中TLR2.4mRNA表達(dá)水平；用聚合酶鏈反應(yīng)限制性片斷長度多態(tài)性(PCR-RFLP)法分析各樣本TLR2 597T/C和TLR4896A/G位點(diǎn)的基因型,比較麻風(fēng)和正常組基因型分布所占比率以及麻風(fēng)病例中各組基因型分別占有比率。用SPSS16.0軟件的方差分析和X2檢驗(yàn)對各組數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)處理。結(jié)果麻風(fēng)組：TLR2mRNA 0.56±0.06,對照組：0.22±0.10,麻風(fēng)組：TLR4mRNA 0.34±0.04,對照組：0.21±0.07,TLR2.4mRNA在麻風(fēng)患者外周血單個(gè)核細(xì)胞中表達(dá)均明顯高于對照組(P0.01)。瘤型麻風(fēng)組：TLR2mRNA 0.62±0.07,TLR4mRNA0.40±0.05;結(jié)核樣型組：TLR2mRNA 0.43±0.11,TLR4mRNA0.21±0.07;對照組：TLR2mRNA 0.22±0.10,TLR4mRNA 0.21±0.07,TLR2mRNA在瘤型和結(jié)核樣型麻風(fēng)中的表達(dá)均高于對照組(P0.05),瘤型麻風(fēng)與結(jié)核樣型組中的表達(dá)相比較未見顯著差異(P0.05)；TLR4mRNA在瘤型麻風(fēng)的表達(dá)明顯高于對照組(P0.05),在結(jié)核樣型麻風(fēng)中的表達(dá)與對照組相比未見顯著差異(P0.05),在瘤型麻風(fēng)中的表達(dá)明顯高于結(jié)核樣型組(P0.05)。家族集聚性組：TLR2mRNA 0.46±0.12,TLR4mRNA 0.27±0.08;非家族集聚性組：TLR2mRNA 0.60±0.08,TLR4mRNA 0.37±0.05;TLR2. 4mRNA在家族集聚性組與非家族集聚性組中的表達(dá)未見顯著差異(P0.05)。TLR2(597T/C)TT、TC、CC三種基因型在麻風(fēng)患者和對照組中的分布頻率分別為43.3%、38.7%、18%和48.0%、42.0%、10.0%,無明顯差異(P0.05)；瘤型和結(jié)核樣型TT、TC、CC基因型所占比率分別為41.7%、38.8%、19.5%和46.8%、38.3%、14.9%,差異無統(tǒng)計(jì)學(xué)意義(P0.05)；家族集聚性和非家族集聚性TT、TC、CC基因型所占比率分別為48.8%、37.2%、14.0%和41.1%、39.3%、19.6%,差異無統(tǒng)計(jì)學(xué)意義(P0.05)。所有研究對象未檢測到TLR4 (896A/G)多態(tài)性,基因型均為896AA野生純合子。結(jié)論TLR2、4mRNA在麻風(fēng)患者外周血單個(gè)核細(xì)胞中的表達(dá)明顯升高,提示TLR2、4可能是麻風(fēng)桿菌的特異識別受體,TLR4可能還在瘤型麻風(fēng)的發(fā)生中起著重要作用,TLR2、4在麻風(fēng)的發(fā)生和病情進(jìn)展中可能起到了一定的作用。TLR2 (597T/C)、TLR4 (896A/G)多態(tài)性與中國貴州畢節(jié)地區(qū)麻風(fēng)無相關(guān)性。
[Abstract]:Objective to study the expression of Toll like receptor (Toll-like receptor,TLR) 2.4mRNA in peripheral blood mononuclear cells (PBMC) of leprosy patients and the single nucleotide polymorphism (single nucleotide polymorphisms, SNP), (SNP) of TLR2-597T/C.TLR4-896A/G gene. To explore the relationship between leprosy and immunity of leprosy patients and its role in leprosy infection. Methods 150 cases of leprosy in Bijie District, Guizhou Province, China were selected, including 103 cases of tumor type, 47 cases of tuberculous type, 43 cases of familial agglomeration, 107 cases of non-familial agglomeration. The expression of TLR2.4mRNA in peripheral blood mononuclear cells (PBMC) of leprosy patients was detected by RT-PCR assay. The genotypes of TLR2 597T/C and TLR4896A/G loci were analyzed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The percentage of genotype distribution in leprosy and normal group and the percentage of genotype in each group were compared. Analysis of variance and X 2 test of SPSS16.0 software were used to deal with the data of each group. Results the expression of TLR2mRNA 0.56 鹵0.06 in leprosy group, 0.22 鹵0.10 in control group, TLR4mRNA 0.34 鹵0.04 in leprosy group and 0.21 鹵0.07 TLR2.4 mRNA in peripheral blood mononuclear cells of leprosy patients were significantly higher than those in control group (P0.01). TLR2mRNA 0.62 鹵0.07TLR4mRNA0.40 鹵0.05in leprosy group, TLR2mRNA 0.43 鹵0.11TLR4mRNA0.21 鹵0.07in tuberculous type group. In control group, the expression of TLR2mRNA 0.22 鹵0.10 TLR4 mRNA 0.21 鹵0.07 TLR2 mRNA in leprosy was significantly higher than that in control group (P0.05), but there was no significant difference between tuberculous leprosy group and tumor-type leprosy group (P0.05). The expression of TLR4mRNA in leprosy was significantly higher than that in control group (P0.05), but the expression of TLR4mRNA in tuberculous leprosy was significantly higher than that in tuberculous leprosy (P0.05). Family agglomeration group: TLR2mRNA 0.46 鹵0.12 TLR4 mRNA 0.27 鹵0.08, TLR2mRNA 0.60 鹵0.08 TLR4 mRNA 0.37 鹵0.05 TLR2. There was no significant difference in the expression of 4mRNA between familial agglomeration group and non-familial agglomeration group (P0.05). The distribution frequency of TLR2 (597T/C) TT,TC,CC genotype in leprosy patients and control group was 43.3% and 38.7%, respectively. There was no significant difference between 18% and 48.0% and 42.0% and 10.0% (P0.05). The percentage of TT,TC,CC genotypes in tumor type and tuberculous type was 41.7% and 38.5%, respectively, and 38.3% and 46.8% in tuberculous type, respectively. The difference was not statistically significant (P0.05). The percentage of TT,TC,CC genotypes of familial agglomeration and non-familial agglomeration were 48.8% and 41.1%, respectively. There was no significant difference between them (P0.05). No TLR4 (896A/G) polymorphism was detected in all subjects and the genotype was 896AA wild homozygote. Conclusion the expression of TLR2,4mRNA in peripheral blood mononuclear cells of leprosy patients is significantly increased, suggesting that TLR2,4 may be a specific recognition receptor of leprosy, and TLR4 may also play an important role in the pathogenesis of leprosy. TLR2,4 may play a role in the occurrence and progression of leprosy, but TLR2 (597T/C), TLR4 (896A/G) polymorphism is not associated with leprosy in Bijie area, Guizhou, China.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2011
【分類號】：R755

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